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151.
Biological control in orchards strongly depends on winter survival of natural enemies, especially in temperate regions. Predacious
phytoseiid mites overwinter on trees or on the ground depending on the characteristics of the species. However, the overwintering
ecology of phytoseiid mites on the ground is less well known than that of those on trees. We investigated the usefulness of
artificial overwintering shelters as a tool for studying the overwintering ecology of phytoseiid mites on the ground. Four
kinds of artificial shelter (shading net, felt, cardboard, and urethane foam) were placed on the ground in an apple orchard
in Korea. Two dominant phytoseiid species, Neoseiulus makuwa (Ehara) and N. womersleyi (Schicha) (Acari: Phytoseiidae), overwintered in the artificial ground shelters, and numbers were highest in the urethane
foam among the four kinds of shelter, and next highest in the shading net. On the other hand, the numbers of phytoseiid mites
collected in the ground vegetation plus soil samples under the ground shelters were not significantly different among the
five shelter treatments, including the no-shelter control. Our results suggest that artificial overwintering shelters are
efficient tools for investigating overwintering ecology of phytoseiid mites on the ground, as well as on trees, in orchards.
Furthermore, the artificial shelters would be good sampling units because they are easily formed into identical sizes and
can be used almost anywhere in the field with less laborious work. We also discuss some implications about the effects of
sheltered structures on the ground on the populations of phytoseiid mites during winter. 相似文献
152.
153.
Yamaguchi Y Okazaki Y Seta N Satoh T Takahashi K Ikezawa Z Kuwana M 《Arthritis research & therapy》2010,12(6):R205
Introduction
Microvasculopathy is one of the characteristic features in patients with systemic sclerosis (SSc), but underlying mechanisms still remain uncertain. In this study, we evaluated the potential involvement of monocytic endothelial progenitor cells (EPCs) in pathogenic processes of SSc vasculopathy, by determining their number and contribution to blood vessel formation through angiogenesis and vasculogenesis. 相似文献154.
Hiroki Obata Noritaka Kawashima Masami Akai Kimitaka Nakazawa Tatsuyuki Ohtsuki 《Journal of electromyography and kinesiology》2010,20(1):55-60
The purpose of this study was to characterize the effects of aging on the stretch reflex in the ankle muscles, and in particular to compare the effects on the ankle dorsi-flexor (tibialis anterior: TA) and the plantar-flexor (soleus: SOL). Stretch reflex responses were elicited in the TA and SOL at rest and during weak voluntary contractions in 20 elderly and 23 young volunteers. The results indicated that, in the TA muscle, the elderly group had a remarkably larger long-latency reflex (LLR), whereas no aging effect was found in the short latency reflex (SLR). These results were very different from those in the SOL muscle, which showed significant aging effects in the SLR and medium latency reflex (MLR), but not in the LLR. Given the fact that the LLR of the TA stretch reflex includes the cortical pathway, it is probable that the effects of aging on the TA stretch reflex involve alterations not only at the spinal level but also at the cortical level. The present results indicate that the stretch reflexes of each of the ankle antagonistic muscles are affected differently by aging, which might have relevance to the neural properties of each muscle. 相似文献
155.
Ohnishi Y Nagase M Ichiyanagi T Kitamoto Y Aimi T 《Applied microbiology and biotechnology》2007,76(5):1069-1078
156.
We use a coarse-grained protein model to characterize the critical nucleus, structural stability, and fibril elongation propensity of Aβ1-40 oligomers for the C2x and C2z quaternary forms proposed by solid-state NMR. By estimating equilibrium populations of structurally stable and unstable protofibrils, we determine the shift in the dominant population from free monomer to ordered fibril at a critical nucleus of ten chains for the C2x and C2z forms. We find that a minimum assembly of 16 monomer chains is necessary to mimic a mature fibril, and show that its structural stability correlates with a plateau in the hydrophobic residue density and a decrease in the likelihood of losing hydrophobic interactions by rotating the fibril subunits. While Aβ1-40 protofibrils show similar structural stability for both C2x and C2z quaternary structures, we find that the fibril elongation propensity is greater for the C2z form relative to the C2x form. We attribute the increased propensity for elongation of the C2z form as being due to a stagger in the interdigitation of the N-terminal and C-terminal β-strands, resulting in structural asymmetry in the presented fibril ends that decreases the amount of incorrect addition to the N terminus on one end. We show that because different combinations of stagger and quaternary structure affect the structural symmetry of the fibril end, we propose that differences in quaternary structures will affect directional growth patterns and possibly different morphologies in the mature fiber. 相似文献
157.
Production of Recombinant β-Hexosaminidase A, a Potential Enzyme for Replacement Therapy for Tay-Sachs and Sandhoff Diseases, in the Methylotrophic Yeast Ogataea minuta
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Hiromi Akeboshi Yasunori Chiba Yoshiko Kasahara Minako Takashiba Yuki Takaoka Mai Ohsawa Youichi Tajima Ikuo Kawashima Daisuke Tsuji Kohji Itoh Hitoshi Sakuraba Yoshifumi Jigami 《Applied microbiology》2007,73(15):4805-4812
Human β-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of α- and β-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount of HexA for a treatment based on enzyme replacement therapy (ERT), recombinant HexA was produced in the methylotrophic yeast Ogataea minuta instead of in mammalian cells, which are commonly used to produce recombinant enzymes for ERT. The problem of antigenicity due to differences in N-glycan structures between mammalian and yeast glycoproteins was potentially resolved by using α-1,6-mannosyltransferase-deficient (och1Δ) yeast as the host. Genes encoding the α- and β-subunits of HexA were integrated into the yeast cell, and the heterodimer was expressed together with its isozymes HexS (αα) and HexB (ββ). A total of 57 mg of β-hexosaminidase isozymes, of which 13 mg was HexA (αβ), was produced per liter of medium. HexA was purified with immobilized metal affinity column for the His tag attached to the β-subunit. The purified HexA was treated with α-mannosidase to expose mannose-6-phosphate (M6P) residues on the N-glycans. The specific activities of HexA and M6P-exposed HexA (M6PHexA) for the artificial substrate 4MU-GlcNAc were 1.2 ± 0.1 and 1.7 ± 0.3 mmol/h/mg, respectively. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern suggested a C-terminal truncation in the β-subunit of the recombinant protein. M6PHexA was incorporated dose dependently into GM2 gangliosidosis patient-derived fibroblasts via M6P receptors on the cell surface, and degradation of accumulated GM2 ganglioside was observed. 相似文献
158.
Comerford I Litchfield W Harata-Lee Y Nibbs RJ McColl SR 《BioEssays : news and reviews in molecular, cellular and developmental biology》2007,29(3):237-247
Directed cell migration is a fundamental component of numerous biological systems and is critical to the pathology of many diseases. Although the importance of secreted chemoattractant factors in providing navigational cues to migrating cells bearing specific chemoattractant receptors is now well-established, how the function of these factors is regulated is not so well understood and may be of key importance to the design of new therapeutics for numerous human diseases. While regulation of migration clearly takes place on a number of different levels, it is becoming clear that so-called 'atypical' receptors play a role in scavenging, or altering the localisation of, chemoattractant molecules such as chemokines and complement components. These receptors do this through binding and/or internalising their chemoattractant ligands without activating signal transduction cascades leading to cell migration. The atypical chemokine receptor family currently comprises the receptors D6, DARC and CCX-CKR. In this review, we discuss the evidence from in vitro and in vivo studies that these receptors play a role in regulating cell migration, and speculate that other orphan receptors may also belong to this family. Furthermore, with the advent of gene therapy on the horizon, the therapeutic potential of these receptors in human disease is also considered. 相似文献
159.
Miyake M Sugano K Kawashima K Ichikawa H Hirabayashi K Kodama T Fujimoto H Kakizoe T Kanai Y Fujimoto K Hirao Y 《Biochemical and biophysical research communications》2007,362(4):865-871
Somatic mutations of the fibroblast growth factor receptor 3 (FGFR3) gene were detected by peptide nucleic acid (PNA)-mediated real-time PCR clamping. Mutation was detected in negative control containing only wild-type DNA due to a misincorporation of dNTPs to PNA binding sites when the amount of template DNA was decreased to 1 ng. Thus, the amount of template DNA was critical determinant of the assay sensitivity in PNA-mediated PCR clamping. Assay conditions were optimized to detect FGFR3 mutations in exons 7, 10, and 15, at a concentration of more than 1% mutated DNA using 50 ng of genomic DNA as the template. Mutations were detected in 12 of 13 (92.3%) tumor tissues and 11 of 13 (84.6%) urine samples from patients with superficial bladder cancer, while no mutations were detected in tissues and/or urine samples from patients with muscle-invasive bladder cancer or chronic cystitis. 相似文献
160.
Murata Y Tsuruzoe K Kawashima J Furukawa N Kondo T Motoshima H Igata M Taketa K Sasaki K Kishikawa H Kahn CR Toyonaga T Araki E 《Biochemical and biophysical research communications》2007,364(2):301-307
Insulin receptor substrate-1 (IRS-1) is the major substrate of both the insulin receptor and the IGF-1 receptor. In this study, we created IRS-1 transgenic (IRS-1-Tg) mice which express human IRS-1 cDNA under control of the mouse IRS-1 gene promoter. In the IRS-1-Tg mice, IRS-1 mRNA expression was significantly increased in almost all tissues, but its protein expression was increased in very limited tissues (epididymal fat and skeletal muscle). IRS-1-Tg mice showed glucose intolerance and significantly enlarged epididymal fat mass, as well as elevated serum TNF-α concentrations. Importantly insulin signaling was significantly attenuated in the liver of IRS-1-Tg mice, which may contribute to the glucose intolerance. Our results suggest that excess IRS-1 expression may not provide a beneficial impact on glucose homeostasis in vivo. 相似文献