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141.
Peng Shi Jun Zhou Huali Song Yujuan Wu Lan Lan Xiangyou Tang Zhengang Ma Charles R. Vossbrinck Bettina Vossbrinck Zeyang Zhou Jinshan Xu 《Ecology and evolution》2020,10(23):13427
The geographic and biological diversity of China has resulted in the differential adaptation of the eastern honeybee, Apis cerana, to these varied habitats. A. cerana were collected from 14 locations in China. Their genomes were sequenced, and nucleotide polymorphisms were identified at more than 9 million sites. Both STRUCTURE and principal component analysis placed the bees into seven groups. Phylogenomic analysis groups the honeybees into many of the same clusters with high bootstrap values (91%–100%). Populations from Tibet and South Yunnan are sister taxa and together represent the earliest diverging lineage included in this study. We propose that the evolutionary origin of A. cerana in China was in the southern region of Yunnan Province and expanded from there into the southeastern regions and into the northeastern mountain regions. The Cold‐Temperate West Sichuan Plateau and Tropical Diannan populations were compared to identify genes under adaptive selection in these two habitats. Pathway enrichment analysis showing genes under selection, including the Hippo signaling pathway, GABAergic pathway, and trehalose‐phosphate synthase, indicates that most genes under selection pressure are involved in the process of signal transduction and energy metabolism. qRT‐PCR analysis reveals that one gene under selection, the AcVIAAT gene, involved in the GABAergic pathway, is responding to cold temperature stress. Through homologous recombination, we show that the AcVIAAT gene is able to replace the CNAG_01904 gene in the fungus Cryptococcus neoformans and that it makes the fungus less sensitive to conditions of oxidative stress and variations in temperature. Our results contribute to our understanding of the evolutionary origin of A. cerana in China and the molecular basis of environmental adaptation. 相似文献
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143.
Microbulbifer hainanensis sp. nov., a moderately halopilic bacterium isolated from mangrove sediment
Cheng Yuping Zhu Suting Guo Chaobo Xie Feilu Jung Dawoon Li Shengying Zhang Weiyan He Shan 《Antonie van Leeuwenhoek》2021,114(7):1033-1042
Antonie van Leeuwenhoek - A new bacterium was successfully isolated from a mangrove sediment sample in Haikou City, Hainan Province, China. The organism is a Gram-negative, rod-shaped, non-motile... 相似文献
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146.
Ana Marcia Escocard de Azevedo Manhaes Fausto Andres Ortiz-Morea Ping He Libo Shan 《植物学报(英文版)》2021,63(1):79-101
As sessile organisms, plants are exposed to pathogen invasions and environmental fluctuations. To overcome the challenges of their surroundings, plants acquire the potential to sense endogenous and exogenous cues, resulting in their adaptability. Hence, plants have evolved a large collection of plasma membrane-resident receptors, including RECEPTOR-LIKE KINASEs(RLKs) and RECEPTOR-LIKE PROTEINs(RLPs) to perceive those signals and regulate plant growth,development, and immunity. The ability of RLKs and RLPs to recognize distinct ligands relies on diverse categories of extracellular domains evolved. Co-regulatory receptors are often required to associate with RLKs and RLPs to facilitate cellular signal transduction. RECEPTOR-LIKE CYTOPLASMIC KINASEs(RLCKs) also associate with the complex, bifurcating the signal to key signaling hubs, such as MITOGEN-ACTIVATED PROTEIN KINASE(MAPK) cascades, to regulate diverse biological processes. Here, we discuss recent knowledge advances in understanding the roles of RLKs and RLPs in plant growth, development, and immunity, and their connection with co-regulatory receptors, leading to activation of diverse intracellular signaling pathways. 相似文献
147.
甘蔗(Saccharum spp.)是世界也是中国主要的糖料作物.由甘蔗黑穗病菌(Sporisorium scitamineum)引起的甘蔗黑穗病是中国甘蔗生产上最主要的病害,每年造成重大损失.分泌蛋白在真菌侵染寄主植物与获取营养及病理过程起重要作用.本实验室在分析甘蔗黑穗病菌基因组与转录组数据时,发现1个与真菌细胞壁降解有关的β-1,6葡聚糖酶前体的基因Ssa4p.为了揭示Ssa4p对甘蔗黑穗病菌致病性和分泌蛋白组的影响,本研究利用CRISPR/Cas9和T-DNA双元载体所构建的甘蔗黑穗菌基因敲除系统,成功获得了Ssa4p基因的失活突变株.ΔSsa4p突变株(Δ35-Ssa4p)与野生型菌株JG36的配合能力降低且致病性减弱.用iTRAQ方法对Δ35-Ssa4p和野生型菌株的分泌蛋白组进行比较分析,共鉴定到蛋白质1430个,其中上调差异表达蛋白质685个,下调差异表达蛋白质745个.GO功能富集分析及KEGG分析表明,差异蛋白主要定位于无膜细胞器和核糖体,其功能包括参与核糖体、氨基糖和核苷酸糖代谢等生物学过程.本研究发现β-1,6葡聚糖前体影响植物病原真菌的分泌蛋白,加深了对真菌致病性调控机制的认识. 相似文献
148.
Jing Cui Kai Shan Qin Yang Yumin Qi Hongyan Qu Jiaqi Li Rong Wang Lingling Jia Wei Chen Ninghan Feng Yong Q. Chen 《Journal of cellular and molecular medicine》2021,25(12):5586-5601
Alternative polarization of macrophages regulates multiple biological processes. While M1-polarized macrophages generally mediate rapid immune responses, M2-polarized macrophages induce chronic and mild immune responses. In either case, polyunsaturated fatty acid (PUFA)-derived lipid mediators act as both products and regulators of macrophages. Prostaglandin E3 (PGE3) is an eicosanoid derived from eicosapentaenoic acid, which is converted by cyclooxygenase, followed by prostaglandin E synthase successively. We found that PGE3 played an anti-inflammatory role by inhibiting LPS and interferon-γ-induced M1 polarization and promoting interleukin-4-mediated M2 polarization (M2a). Further, we found that although PGE3 had no direct effect on the growth of prostate cancer cells in vitro, PGE3 could inhibit prostate cancer in vivo in a nude mouse model of neoplasia. Notably, we found that PGE3 significantly inhibited prostate cancer cell growth in a cancer cell-macrophage co-culture system. Experimental results showed that PGE3 inhibited the polarization of tumour-associated M2 macrophages (TAM), consequently producing indirect anti-tumour activity. Mechanistically, we identified that PGE3 regulated the expression and activation of protein kinase A, which is critical for macrophage polarization. In summary, this study indicates that PGE3 can selectively promote M2a polarization, while inhibiting M1 and TAM polarization, thus exerting an anti-inflammatory effect and anti-tumour effect in prostate cancer. 相似文献
149.
Shan Jiang Wenjing Xu Zhenzhen Chen Changting Cui Xiaofang Fan Jun Cai Yongsheng Gong Bin Geng 《Journal of cellular and molecular medicine》2021,25(7):3437-3448
Hyperhomocysteinaemia (HHcy)-impaired endothelial dysfunction including endoplasmic reticulum (ER) stress plays a crucial role in atherogenesis. Hydrogen sulphide (H2S), a metabolic production of Hcy and gasotransmitter, exhibits preventing cardiovascular damages induced by HHcy by reducing ER stress, but the underlying mechanism is unclear. Here, we made an atherosclerosis with HHcy mice model by ApoE knockout mice and feeding Pagien diet and drinking L-methionine water. H2S donors NaHS and GYY4137 treatment lowered plaque area and ER stress in this model. Protein disulphide isomerase (PDI), a modulation protein folding key enzyme, was up-regulated in plaque and reduced by H2S treatment. In cultured human aortic endothelial cells, Hcy dose and time dependently elevated PDI expression, but inhibited its activity, and which were rescued by H2S. H2S and its endogenous generation key enzyme-cystathionine γ lyase induced a new post-translational modification-sulfhydration of PDI. Sulfhydrated PDI enhanced its activity, and two cysteine-terminal CXXC domain of PDI was identified by site mutation. HHcy lowered PDI sulfhydration association ER stress, and H2S rescued it but this effect was blocked by cysteine site mutation. Conclusively, we demonstrated that H2S sulfhydrated PDI and enhanced its activity, reducing HHcy-induced endothelial ER stress to attenuate atherosclerosis development. 相似文献
150.
Wei Liu Chunyu Wang Shengli Wang Kai Zeng Shan Wei Ning Sun Ge Sun Manlin Wang Renlong Zou Wensu Liu Lin Lin Huijuan Song Zining Jin Yue Zhao 《International journal of biological sciences》2021,17(1):188
Androgen receptor (AR) and its variants play vital roles in development and progression of prostate cancer. To clarify the mechanisms involved in the enhancement of their actions would be crucial for understanding the process in prostate cancer and castration-resistant prostate cancer transformation. Here, we provided the evidence to show that pre-mRNA processing factor 6 (PRPF6) acts as a key regulator for action of both AR full length (AR-FL) and AR variant 7 (AR-V7), thereby participating in the enhancement of AR-FL and AR-V7-induced transactivation in prostate cancer. In addition, PRPF6 is recruited to cis-regulatory elements in AR target genes and associates with JMJD1A to enhance AR-induced transactivation. PRPF6 also promotes expression of AR-FL and AR-V7. Moreover, PRPF6 depletion reduces tumor growth in prostate cancer-derived cell lines and results in significant suppression of xenograft tumors even under castration condition in mouse model. Furthermore, PRPF6 is obviously highly expressed in human prostate cancer samples. Collectively, our results suggest PRPF6 is involved in enhancement of oncogenic AR signaling, which support a previously unknown role of PRPF6 during progression of prostate cancer and castration-resistant prostate cancers. 相似文献