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991.
Miho Inoue-Murayama Osamu Takenaka Yuichi Murayama 《Primates; journal of primatology》1998,39(2):217-224
Human D4 dopamine receptor (D4DR) is polymorphic in terms of the repeat, numbers of the 48-base pairs (bp) sequence located
in the third cytoplasmic loop of the receptor. The repeated sequence and its polymorphism in D4DR genes have also been identified
in higher non-human primates, suggesting that the structure of D4DR has been maintained during primate evolution. To clarify
the origin and divergence of the polymorphism in the D4DR gene, we determined the nucleotide sequence of this region of the
D4DR gene in several species of prosimians and the tree shrew, a species which is closely related to primates. Prosimians
except the tarsier had one or two unit(s) of the 48-bp sequence, and conserved sequences were recognized in most of the units
of the prosimians. The tree shrew had only one unit of the 48-bp sequence, and its sequence was 71–75% identical to those
of the nuits of galago, loris, and lemur. These findings suggest that the ancestral primate presumably had one 48-bp unit,
and duplication of the unit occurred at the stage of prosimians. Tarsiers appeared to be distinct from other prosimians and
simians because of the high repeat numbers of units and their sequences. 相似文献
992.
993.
In angiosperms, the expression of the cab gene that encodesthe chlorophyll a/b-binding protein of PSII is light-regulated.However, the pine cab gene is expressed in a light-independentbut cell-type-specific manner. In the present study, the cab-6promoter (1.7 kbp) from pine was fused to a -glucuronidase (GUS)reporter gene and the chimeric gene was introduced into riceprotoplasts by electroporation. The GUS expression was studiedin the resultant transgenic rice plants. Expression of GUS ata substantial level was confirmed in primary leaves of dark-germinatedrice seedlings, and no obvious effect of light on the GUS activitywas observed. The expression of GUS was restricted to photosynthetictissues. The pine cab-6 promoter is, thus, sufficient for inductionof light-independent but cell-type-specific expression in cellsof a monocot, as is the case in the original pine cells. (Received December 17, 1993; Accepted April 22, 1994) 相似文献
994.
Yasumaru Hatanaka Makoto Hashimoto Yuichi Kanaoka 《Bioorganic & medicinal chemistry》1994,2(12):1367-1373
The synthesis of a p-[(3-trifluoromethyl)diazirine-3-yl]benzoic acid derivative is described as a new carbene generating heterobifunctional cross-linking reagent. The cross-linker carries a biotin moiety in order to make use of avidin—biotin technology for specific manipulation of cross-linked components. To evaluate the ability of this reagent, the inter-subunit cross-linking of egg-white avidin tetramer was investigated. As a typical application of avidin—biotin technology for cross-linking experiments, a chemiluminescent detection method was examined to identify photobiotinylated components. A cross-linked dimeric product with an apparent molecular mass of 38 kDa was clearly visualized by the combined use of a horseradish peroxidase—streptavidin conjugate and a luminol-based chemiluminescent system. 相似文献
995.
996.
997.
Kenji Kai Hideyuki Ohnishi Akinori Kiba Kouhei Ohnishi Yasufumi Hikichi 《Bioscience, biotechnology, and biochemistry》2016,80(3):440-444
Ralfuranones, aryl-furanone secondary metabolites, are involved in the virulence of Ralstonia solanacearum in solanaceous plants. Ralfuranone I (6) has been suggested as a biosynthetic precursor for other ralfuranones; however, this conversion has not been confirmed. We herein investigate the biosynthesis of ralfuranones using feeding experiments with ralfuranone I (6) and its putative metabolite, ralfuranone B (2). The results obtained demonstrated that the biosynthesis of ralfuranones proceeded in enzymatic and non-enzymatic manners. 相似文献
998.
999.
C55 bacteriocin produced by ETB‐plasmid positive Staphylococcus aureus strains is a key factor for competition with S. aureus strains 下载免费PDF全文
Miki Kawada‐Matsuo Fariha Shammi Yuichi Oogai Norifumi Nakamura Motoyuki Sugai Hitoshi Komatsuzawa 《Microbiology and immunology》2016,60(3):139-147
1000.