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161.
Photosynthetic generation of reducing power makes cyanobacteria an attractive host for biochemical reduction compared to cell‐free and heterotrophic systems, which require burning of additional resources for the supply of reducing equivalent. Here, using xylitol synthesis as an example, efficient uptake and reduction of xylose photoautotrophically in Synechococcus elongatus PCC7942 are demonstrated upon introduction of an effective xylose transporter from Escherichia coli (Ec‐XylE) and the NADPH‐dependent xylose reductase from Candida boidinii (Cb‐XR). Simultaneous activation of xylose uptake and matching of cofactor specificity enabled an average xylitol yield of 0.9 g g?1 xylose and a maximum productivity of about 0.15 g L?1 day?1 OD?1 with increased level of xylose supply. While long‐term cellular maintenance still appears challenging, high‐density conversion of xylose to xylitol using concentrated resting cell further pushes the titer of xylitol formation to 33 g L?1 in six days with 85% of maximum theoretical yield. While the results show that the unknown dissipation of xylose can be minimized when coupled to a strong reaction outlet, it remains to be the major hurdle hampering the yield despite the reported inability of cyanobacteria to metabolize xylose. 相似文献
162.
163.
Minmin Du Ke Zhou Yuanyuan Liu Lei Deng Xiaoyue Zhang Lihao Lin Ming Zhou Wei Zhao Changlong Wen Jiayi Xing Chang‐Bao Li Chuanyou Li 《The Plant journal : for cell and molecular biology》2020,102(5):1090-1100
Incorporating male sterility into hybrid seed production reduces its cost and ensures high varietal purity. Despite these advantages, male‐sterile lines have not been widely used to produce tomato (Solanum lycopersicum) hybrid seeds. We describe the development of a biotechnology‐based breeding platform that utilized genic male sterility to produce hybrid seeds. In this platform, we generated a novel male‐sterile tomato line by clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9)‐mediated mutagenesis of a stamen‐specific gene SlSTR1 and devised a transgenic maintainer by transforming male‐sterile plants with a fertility‐restoration gene linked to a seedling‐colour gene. Offspring of crosses between a hemizygous maintainer and the homozygous male‐sterile plant segregated into 50% non‐transgenic male‐sterile plants and 50% male‐fertile maintainer plants, which could be easily distinguished by seedling colour. This system has great practical potential for hybrid seed breeding and production as it overcomes the problems intrinsic to other male‐sterility systems and can be easily adapted for a range of tomato cultivars and diverse vegetable crops. 相似文献
164.
165.
Ziyu He Zhu Chen Miduo Tan Sauli Elingarami Yuan Liu Taotao Li Yan Deng Nongyue He Song Li Juan Fu Wen Li 《Cell proliferation》2020,53(7)
Breast cancer has seriously been threatening physical and mental health of women in the world, and its morbidity and mortality also show clearly upward trend in China over time. Through inquiry, we find that survival rate of patients with early‐stage breast cancer is significantly higher than those with middle‐ and late‐stage breast cancer, hence, it is essential to conduct research to quickly diagnose breast cancer. Until now, many methods for diagnosing breast cancer have been developed, mainly based on imaging and molecular biotechnology examination. These methods have great contributions in screening and confirmation of breast cancer. In this review article, we introduce and elaborate the advances of these methods, and then conclude some gold standard diagnostic methods for certain breast cancer patients. We lastly discuss how to choose the most suitable diagnostic methods for breast cancer patients. In general, this article not only summarizes application and development of these diagnostic methods, but also provides the guidance for researchers who work on diagnosis of breast cancer. 相似文献
166.
空气中污染物及病菌的浓度直接影响人类健康。在污染源不变的情况下,有效通风决定了空气质量的优劣。因此,建立有效通风的定量判定指标,并利用此指标对特定区域空气质量进行实时评价,引导居民选择健康的室外活动场所成为迫切需要解决的问题。本研究采用雷诺平均Navier-Stokes(RANS)方法建立了基于计算流体力学(CFD)技术的城市空气污染模拟系统,研究了风速、污染物浓度和污染物扩散效率之间的关系。在此基础上,借助实时气象数据,对城市广场空间不同时段进行了风场模拟及空气质量评价。结果表明: 行人高度(1.5 m)空气中污染物有效扩散的临界风速值为1.0 m·s-1,此指标可作为某一具体区域空气质量评价标准。参照此判定指标,链接实时天气系统,通过模拟得到的行人高度风速分布,可实时、可视化地显示该场所空气质量优劣分布,实现公平、效率、合理地利用城市空间资源,为人们选择健康的室外活动场地提供指引,为公众疾病预防和健康促进提供技术和手段。 相似文献
167.
新疆吉木萨尔县蝴蝶群落多样性 总被引:1,自引:0,他引:1
蝴蝶作为指示生物, 被广泛地应用于生物多样性监测及环境质量评估。探究新疆吉木萨尔县蝴蝶群落多样性, 可为当地蝴蝶多样性的保护及环境监测提供基础数据。本研究采用样线法在新疆吉木萨尔县选取山前荒漠、农田、山地草原、山地森林、亚高山草甸5种不同的生境类型, 对蝴蝶种类和群落多样性进行调查。共记录蝴蝶4,401号, 隶属于7科26属38种。其中蛱蝶科有9属12种, 为优势科; 粉蝶科的个体数最多, 占比55.01%; 绢蝶科、凤蝶科和弄蝶科的种类数和个体数最少, 均为单科种, 是该地区的稀有类群。对不同生境蝴蝶群落多样性和相似度分析比较的结果显示: 5种生境中多样性指数从高到低依次为亚高山草甸、山地森林、山地草原、农田及山前荒漠, 其中山地森林和亚高山草甸的相似性系数较高, 达到0.77, 山前荒漠和山地草原的相似性系数最低, 为0.37。蝴蝶物种数及多样性指数随海拔的增加呈上升趋势。蝴蝶群落随月份发生变化, 蝴蝶种类和数量在5月发生、7月达到峰值。蝶类个体数在3年内呈下降趋势。研究结果表明, 蝴蝶物种的组成和多样性与生境类型具有密切联系, 保护生态环境, 维持该地区植物群落的多样性、降低人为干扰程度是保护蝶类多样性的关键。 相似文献
168.
2019年4-6月,在江苏省西南部句容市郭庄镇虬山,首次发现二色仙人掌(Opuntia cespitosa Rafinesque)归化种群。该种群主要生长在海拔46~90 m的废弃采石场上,分布范围东西长510 m,南北宽260 m,面积约13.26 hm2。二色仙人掌开花结实的成年植株共计910棵,其中最大灌木平卧茎长轴上叶状茎10个,树龄可达10 a,大概在2010年就生长于虬山。在句容虬山采石场关闭时,采石场工作人员可能将盆栽的二色仙人掌就地丢弃,从而导致该归化种群的发展。比较了二色仙人掌与中国仙人掌属已知其余4种归化植物的形态特征,并编制了分种检索表。此外,讨论了二色仙人掌的传播机制及其入侵风险。 相似文献
169.
Jian Zhao Yanci Wen Songming Zhu Jinyun Ye Junjie Zhu Zhangying Ye Alex Jordan 《Proceedings. Biological sciences / The Royal Society》2020,287(1938)
Foraging animals must balance benefits of food acquisition with costs induced by a post-prandial reduction in performance. Eating to satiation can lead to a reduction in locomotor and escape performance, which increases risk should a threat subsequently arises, but limiting feeding behaviour may be maladaptive if food intake is unnecessarily reduced in the prediction of threats that do not arise. The efficacy of the trade-off between continued and interrupted feeding therefore relies on information about the future risk, which is imperfect. Here, we find that black carp (Mylopharyngodon piceus) can balance this trade-off using an a posteriori strategy; by eating to satiation but regurgitating already ingested food when a threat arises. While degrees of satiation (DS) equal to or greater than 60% reduce elements of escape performance (turning angle, angular velocity, distance moved, linear velocity), at 40% DS or lower, performance in these tasks approaches levels comparable to that at 0% satiation. After experiencing a chasing event, we find that fish are able to regurgitate already ingested food, thereby changing the amount of food in their gastrointestinal tract to consistent levels that maintain high escape performance. Remarkably, regurgitation results in degrees of satiation between 40 and 60% DS, regardless of whether they had previously fed to 40, 60 or 100% DS. Using this response, fish are able to maximize food intake, but regurgitate extra food to maintain escape performance when they encounter a threat. This novel strategy may be effective for continual grazers and species with imperfect information about the level of threat in their environment. 相似文献
170.
The effect of grazing on winter survival of midday gerbil (Meriones meridianus) of different genders
SuWen Yang Shuai Yuan XiaoDong Wu Rong Zhang XiuXian Yue Yu Ji LinLin Li Xin Li HePing Fu 《Ecology and evolution》2020,10(21):12395
The objective of this study was to investigate the effects of grazing on midday gerbil (Meriones meridianus) population characteristics and survival of animals of different genders. The experiment used a randomized complete block design and was conducted in Alxa Left Banner, Inner Mongolia, China, in 2002 (The agricultural reclamation plots set up in 1994). From April 2006 to October 2010, midday gerbils were live‐trapped in 3 light grazing plots, 3 overgrazed plots, and 3 grazing exclusion plots. The quantity of vegetation was investigated in the two different grazing intensity areas and grazing exclusion area to determine the relationship between gerbils and plant food availability. The results suggested that there was higher gerbil density, individual body mass, and daily body mass growth rate in the grazing exclusion sites than the other sites across the whole year. Females had higher survival in grazing exclusion areas than in other treatments, but the males’ survival showed the opposite pattern. Our results indicated that grazing negatively influenced the midday gerbil population by reducing food availability. Grazing influenced the survival rates of male midday gerbils positively, but had negative effects on females. The reason for gendered differences in survival rates of midday gerbils requires further investigation. 相似文献