全文获取类型
收费全文 | 314篇 |
免费 | 16篇 |
国内免费 | 57篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 19篇 |
2021年 | 18篇 |
2020年 | 7篇 |
2019年 | 8篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 10篇 |
2015年 | 16篇 |
2014年 | 19篇 |
2013年 | 28篇 |
2012年 | 34篇 |
2011年 | 42篇 |
2010年 | 15篇 |
2009年 | 11篇 |
2008年 | 16篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 13篇 |
2004年 | 18篇 |
2003年 | 6篇 |
2002年 | 13篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1997年 | 9篇 |
1996年 | 1篇 |
1995年 | 4篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1985年 | 1篇 |
排序方式: 共有387条查询结果,搜索用时 15 毫秒
151.
Allium ascalonicum L,在我国的邦名比较混乱,而且它与洋葱、葱的亲缘关系不清。作者认为,它在我国的邦名应规范为胡葱,同时,从性状比较的角度研究胡葱与洋葱、葱之间的亲缘关系。结果表明,胡葱的植物学特征和生物学特性介于洋葱和葱之间。 相似文献
152.
土壤-植物系统中多环芳烃和重金属的行为研究 总被引:14,自引:0,他引:14
对土壤中多环芳烃和重金属的行为研究表明,与对照相比,0—20cm以上表土层存在多环芳烃和重金属积累,20cm以下土层未发现积累;与春、秋两次采样结果相比,土壤中多环芳烃的含量有所下降,表明土壤微生物对多环芳烃有一定降解作用,且其降解程度与土壤-植物系统的生态结构有关.菲在地下水中检出浓度较高,表明这一污染物有向下迁移的可能性.此外,柳树对土壤中重金属Cd的积累有明显的削减与净化作用.本研究表明,严格限制污水中多环芳烃和重金属的污染负荷以及设计合理的生态结构是避免多环芳烃和重金属在土壤中积累的关键. 相似文献
153.
糖化酶基因的克隆和在酿酒酵母中表达 总被引:1,自引:1,他引:1
以穿梭质粒pLCl为载体,大肠杆菌C600为受体构建了扣囊拟内孢霉(Endomycopsis fibuligera)Sau34基因文库。从基因文库中提取重组质粒DNA,转化酿酒酵母BJ1991,选出具有淀粉水解酶活性的转化子,它含有编码扣囊拟内孢霉胞外糖化酶的基因片段,能发酵淀粉。琼脂糖凝胶电泳证明所插入DNA片段为5.3kb,SDS-聚丙烯酰胺凝胶电泳测得糖化酶的分子量为54000。酶活最适温度为50℃,最适pH为5.5。 相似文献
154.
除草剂胁迫对空心莲子草叶甲种群的影响及应对策略 总被引:3,自引:0,他引:3
为探讨除草剂胁迫对空心莲子草叶甲种群的影响,寻求合理施用除草剂,有效保护叶甲,提高其控草效果的应对策略,利用叶片残留法测试了在草甘膦、农达、水花生净与35%苄·丁可湿粉等除草剂胁迫下空心莲子草叶甲卵孵化率,幼虫、成虫存活率以及蛹羽化率。结果表明被测试的4种除草剂均不同程度地降低空心莲子草叶甲卵孵化率,幼虫与成虫存活率及蛹羽化率,特别是水花生净28.0、35.0 g/L两种浓度处理,35%苄·丁可湿粉4.0 g/L浓度处理显著降低卵孵化率;农达13.4 g/L浓度处理显著降低1龄前期幼虫存活率,农达40.1 g/L与 53.4 g/L两种浓度处理显著降低2龄前期幼虫存活率;水花生净28.0 g/L浓度处理显著降低3龄前期幼虫存活率;草甘膦97.6 g/L与 122.0 g/L浓度处理、农达26.7 g/L与66.8 g/L浓度处理、35%苄·丁可湿粉2.0 g/L浓度处理显著降低后期蛹的羽化率;35%苄·丁可湿粉1.0 g/L浓度处理显著降低羽化后3 d雌虫的存活率。除草剂除直接杀死空心莲子草叶甲,降低其种群数量外,使叶甲失去食物与避护所,快速地引起叶甲种群崩溃。建议合理施药,并在施药时,随机留若干小斑块不施药,保护叶甲,可望提高其田间控草效果。 相似文献
155.
Diagnosis of liver cancer using HPLC-based metabonomics avoiding false-positive result from hepatitis and hepatocirrhosis diseases 总被引:19,自引:0,他引:19
Yang J Xu G Zheng Y Kong H Pang T Lv S Yang Q 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,813(1-2):59-65
Metabonomics, the study of metabolites and their roles in various disease states, is a novel methodology arising from the post-genomics era. This methodology has been applied in many fields. Current metabonomics practice has relied on mass spectrometry (MS), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) to analyze metabolites. In this study, a novel approach of using high-performance liquid chromatography (HPLC) in conjunction with developed software was employed. Using the principal components analysis method (PCA), all (113) peaks of urinary metabolites with a cis-diol structure from patients with hepatitis and hepatocirrhosis were compared to those from liver cancer patients. The results showed that the metabonomics-PCA method might be useful to differentiate between patients with hepatocirrhosis and hepatitis from patients with liver cancer while lowering false-positive rate. These findings also suggest that a subset of the urinary nucleosides identified with metabonomics correlate better with cancer diagnosis than the traditional single tumor marker alpha-fetoprotein (AFP). 相似文献
156.
污灌土壤中多环芳烃(PAHs)的积累与动态变化研究 总被引:43,自引:5,他引:43
对污灌土壤中 1 4种多环芳烃的分析表明 ,各灌区土壤中 PAHs的积累一般以渠首最高 ,渠中次之 ,渠尾含量与对照相当 .但在沈抚石油灌区上、中和下游土壤中均有PAHs的积累 .此外 ,水稻生长期污灌可明显增加土壤中 PAHs的总量 ,各单一污染物的增、减趋势有所不同 . 相似文献
157.
Tuo Ma Kai Gong Yufang Yan Bo Song Xiufang Zhang Yandao Gong 《Biochemical and biophysical research communications》2012,426(2):196-202
Mitochondrial malfunction and calcium dyshomeostasis are early pathological events considered as important features of the Alzheimer’s disease (AD) brain. Recent studies have suggested mitochondrion as an active regulator of Ca2+ signaling based on its calcium buffering capacity. Herein, we investigated the mitochondrial involvement in the modulation of store-operated calcium entry (SOCE) in neural 2a (N2a) transgenic AD model cells. Results showed that SOCE was significantly depressed in N2a cells transfected with wild-type human APP695 (N2a APPwt) compared with empty vector control (N2a WT) cells. Pharmacological manipulation with mitochondrial function blockers, such as FCCP, RuR, or antimycin A/oligomycin, could inhibit mitochondrial calcium handling, and then impair SOCE pathway in N2a WT cells. Furthermore, mitochondria of N2a APPwt cells exhibited more severe swelling in response to Ca2+, which is an indication of mitochondrial membrane permeability transition (MPT), than the wild-type controls. Additionally, treatment with cyclosporin A, a potent inhibitor of cyclophilin D, which can block MPT, could significantly restore the attenuated SOCE in N2a APPwt cells. Therefore, inhibition of cyclophilin D might be a therapeutic strategy for Alzheimer’s disease. 相似文献
158.
重庆八腱索线虫新种的描述 总被引:2,自引:0,他引:2
本文记述了寄生于中华按蚊幼虫体内的一种索科(Mermithidae)线虫,属八腱索属中的一新种——重庆八腱索线虫(Octomyomermis chongqingensis sp.nov.)。根据其形态学特征进行测量和描述,并与本属内的近似种作了鉴别比较。本属在我国尚属首次记录。 相似文献
159.
Chronic morphine treatment promotes specific Th2 cytokine production by murine T cells in vitro via a Fas/Fas ligand-dependent mechanism 总被引:2,自引:0,他引:2
Greeneltch KM Kelly-Welch AE Shi Y Keegan AD 《Journal of immunology (Baltimore, Md. : 1950)》2005,175(8):4999-5005
Improper homeostasis of Th1 and Th2 cell differentiation can promote pathological immune responses such as autoimmunity and asthma. A number of factors govern the development of these cells including TCR ligation, costimulation, death effector expression, and activation-induced cell death (AICD). Although chronic morphine administration has been shown to selectively promote Th2 development in unpurified T cell populations, the direct effects of chronic morphine on Th cell skewing and cytokine production by CD4(+) T cells have not been elucidated. We previously showed that morphine enhances Fas death receptor expression in a T cell hybridoma and human PBL. In addition, we have demonstrated a role for Fas, Fas ligand (FasL), and TRAIL in promoting Th2 development via killing of Th1 cells. Therefore, we analyzed whether the ability of morphine to affect Th2 cytokine production was mediated by regulation of Fas, FasL, and TRAIL expression and AICD directly in purified Th cells. We found that morphine significantly promoted IL-4 and IL-13 production but did not alter IL-5 or IFN-gamma. Furthermore, morphine enhanced the mRNA expression of Fas, FasL and TRAIL and promoted Fas-mediated AICD of CD4(+) T cells. Additionally, blockade of Fas/FasL interaction by anti-FasL inhibited the morphine-induced production of IL-4 and IL-13 and AICD of CD4(+) T cells. These results suggest that morphine preferentially enhances Th2 cell differentiation via killing of Th1 cells in a Fas/FasL-dependent manner. 相似文献
160.
Novel five-member thio-heterocyclic fused naphthalimides with aminoalkyl side chains were designed, synthesized and evaluated. These compounds have high Scatchard binding constants. They could damage DNA (supercoiled pBR322) from form I (closed) to II (nicked) at a concentration as low as 10 microM and from form I to form III at a concentration of 50 microM. The results implied that the influence of intercalating ability of chromophores on photocleaving ability of photocleavers depended on the mechanism of photocleavage. 相似文献