全文获取类型
收费全文 | 17001篇 |
免费 | 1838篇 |
国内免费 | 2224篇 |
出版年
2024年 | 66篇 |
2023年 | 269篇 |
2022年 | 568篇 |
2021年 | 924篇 |
2020年 | 741篇 |
2019年 | 853篇 |
2018年 | 775篇 |
2017年 | 611篇 |
2016年 | 746篇 |
2015年 | 1101篇 |
2014年 | 1264篇 |
2013年 | 1374篇 |
2012年 | 1597篇 |
2011年 | 1439篇 |
2010年 | 990篇 |
2009年 | 845篇 |
2008年 | 1009篇 |
2007年 | 888篇 |
2006年 | 748篇 |
2005年 | 652篇 |
2004年 | 626篇 |
2003年 | 596篇 |
2002年 | 592篇 |
2001年 | 325篇 |
2000年 | 268篇 |
1999年 | 253篇 |
1998年 | 157篇 |
1997年 | 132篇 |
1996年 | 101篇 |
1995年 | 69篇 |
1994年 | 80篇 |
1993年 | 51篇 |
1992年 | 52篇 |
1991年 | 50篇 |
1990年 | 38篇 |
1989年 | 37篇 |
1988年 | 26篇 |
1987年 | 25篇 |
1986年 | 29篇 |
1985年 | 14篇 |
1984年 | 14篇 |
1983年 | 12篇 |
1982年 | 12篇 |
1981年 | 7篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1973年 | 3篇 |
1969年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
141.
Yue Li Steven M. Shea Christine H. Lorenz Hangyi Jiang Ming-Chung Chou Susumu Mori 《PloS one》2013,8(10)
Due to the high sensitivity of diffusion tensor imaging (DTI) to physiological motion, clinical DTI scans often suffer a significant amount of artifacts. Tensor-fitting-based, post-processing outlier rejection is often used to reduce the influence of motion artifacts. Although it is an effective approach, when there are multiple corrupted data, this method may no longer correctly identify and reject the corrupted data. In this paper, we introduce a new criterion called “corrected Inter-Slice Intensity Discontinuity” (cISID) to detect motion-induced artifacts. We compared the performance of algorithms using cISID and other existing methods with regard to artifact detection. The experimental results show that the integration of cISID into fitting-based methods significantly improves the retrospective detection performance at post-processing analysis. The performance of the cISID criterion, if used alone, was inferior to the fitting-based methods, but cISID could effectively identify severely corrupted images with a rapid calculation time. In the second part of this paper, an outlier rejection scheme was implemented on a scanner for real-time monitoring of image quality and reacquisition of the corrupted data. The real-time monitoring, based on cISID and followed by post-processing, fitting-based outlier rejection, could provide a robust environment for routine DTI studies. 相似文献
142.
Ming Xu Vladimir V. Ermolenkov Vladimir N. Uversky Igor K. Lednev 《Journal of biophotonics》2008,1(3):215-229
Amyloid fibrils are associated with numerous degenerative diseases. The molecular mechanism of the structural transformation of native protein to the highly ordered cross‐β structure, the key feature of amyloid fibrils, is under active investigation. Conventional biophysical methods have limited application in addressing the problem because of the heterogeneous nature of the system. In this study, we demonstrated that deep‐UV resonance Raman (DUVRR) spectroscopy in combination with circular dichroism (CD) and intrinsic tryptophan fluorescence allowed for quantitative characterization of protein structural evolution at all stages of hen egg white lysozyme fibrillation in vitro. DUVRR spectroscopy was found to be complimentary to the far‐UV CD because it is (i) more sensitive to β ‐sheet than to α ‐helix, and (ii) capable of characterizing quantitatively inhomogeneous and highly light‐scattering samples. In addition, phenylalanine, a natural DUVRR spectroscopic biomarker of protein structural rearrangements, exhibited substantial changes in the Raman cross section of the 1000‐cm–1 band at various stages of fibrillation. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
143.
Peiyu Liu Yan Liu Xiang Zhao Andrew P. Roberts Heng Zhang Yue Zheng Fuxian Wang Lushan Wang Nicolas Menguy Yongxin Pan Jinhua Li 《Environmental microbiology》2021,23(2):1115-1129
Magnetotactic bacteria (MTB) are diverse prokaryotes that produce magnetic nanocrystals within intracellular membranes (magnetosomes). Here, we present a large-scale analysis of diversity and magnetosome biomineralization in modern magnetotactic cocci, which are the most abundant MTB morphotypes in nature. Nineteen novel magnetotactic cocci species are identified phylogenetically and structurally at the single-cell level. Phylogenetic analysis demonstrates that the cocci cluster into an independent branch from other Alphaproteobacteria MTB, that is, within the Etaproteobacteria class in the Proteobacteria phylum. Statistical analysis reveals species-specific biomineralization of magnetosomal magnetite morphologies. This further confirms that magnetosome biomineralization is controlled strictly by the MTB cell and differs among species or strains. The post-mortem remains of MTB are often preserved as magnetofossils within sediments or sedimentary rocks, yet paleobiological and geological interpretation of their fossil record remains challenging. Our results indicate that magnetofossil morphology could be a promising proxy for retrieving paleobiological information about ancient MTB. 相似文献
144.
145.
The Indian Ocean is the least known ocean in the world with the biogeography of marine species in the West Indian Ocean (WIO) understudied. The hydrography of WIO is characterized by four distinct oceanographic systems and there were few glacial refugia formations in the WIO during the Pleistocene. We used the widely distributed intertidal barnacle Tetraclita to test the hypothesis that the distribution and connectivity of intertidal animals in the WIO are determined by the major oceanographic regime but less influenced by historical events such as Pleistocene glaciations. Tetraclita were studied from 32 locations in the WIO. The diversity and distribution of Tetraclita species in the Indian Ocean were examined based on morphological examination and sequence divergence of two mitochondrial genes (12S rDNA and COI) and one nuclear gene (histone 3, H3). Divergence in DNA sequences revealed the presence of seven evolutionarily significant units (ESUs) of Tetraclita in WIO, with most of them recognized as valid species. The distribution of these ESUs is closely tied to the major oceanographic circulation systems. T. rufotincta is distributed in the Monsoonal Gyre. T. ehsani is present in the Gulf of Oman and NW India. Tetraclita sp. nov. is associated with the Hydrochemical Front at 10°S latitude. T. reni is confined to southern Madagascan and Mauritian waters, influenced by the West Wind Drift. The endemic T. achituvi is restricted to the Red Sea. Tetraclita serrata consists of two ESUs (based on mtDNA analysis) along the east to west coast of South Africa. The two ESUs could not be distinguished from morphological analysis and nuclear H3 sequences. Our results support that intertidal species in the West Indian Ocean are associated with each of the major oceanographic circulation systems which determine gene flow. Geographical distribution is, however, less influenced by the geological history of the region. 相似文献
146.
Liu Minliang Liang Liang Liu Haofei Zhang Ming Martin Caitlin Sun Wei 《Biomechanics and modeling in mechanobiology》2019,18(2):387-398
Biomechanics and Modeling in Mechanobiology - It is well known that residual deformations/stresses alter the mechanical behavior of arteries, e.g., the pressure–diameter curves. In an effort... 相似文献
147.
Tai‐An Chiang Yu‐Lin Yang Ya‐Ying Yang Min‐Hsiu Hu Pei‐Fen Wu Shu‐Fen Liu Ruay‐Ming Huang Tung‐Nan Liao Chien‐Ya Hung Tsung‐Jen Hung Tao‐Chen Lee 《Journal of cellular biochemistry》2010,109(4):663-671
Hyperosmolarity plays an essential role in the pathogenesis of diabetic tubular fibrosis. However, the mechanism of the involvement of hyperosmolarity remains unclear. In this study, mannitol was used to evaluate the effects of hyperosmolarity on a renal distal tubule cell line (MDCK). We investigated transforming growth factor‐β receptors and their downstream fibrogenic signal proteins. We show that hyperosmolarity significantly enhances the susceptibility to exogenous transforming growth factor (TGF)‐β1, as mannitol (27.5 mM) significantly enhanced the TGF‐β1‐induced increase in fibronectin levels compared with control experiments (5.5 mM). Specifically, hyperosmolarity induced tyrosine phosphorylation on TGF‐β RII at 336 residues in a time (0–24 h) and dose (5.5–38.5 mM) dependent manner. In addition, hyperosmolarity increased the level of TGF‐β RI in a dose‐ and time‐course dependent manner. These observations may be closely related to decreased catabolism of TGF‐β RI. Hyperosmolarity significantly downregulated the expression of an inhibitory Smad (Smad7), decreased the level of Smurf 1, and reduced ubiquitination of TGF‐β RI. In addition, through the use of cycloheximide and the proteasome inhibitor MG132, we showed that hyperosmolarity significantly increased the half‐life and inhibited the protein level of TGF‐β RI by polyubiquitination and proteasomal degradation. Taken together, our data suggest that hyperosmolarity enhances cellular susceptibility to renal tubular fibrosis by activating the Smad7 pathway and increasing the stability of type I TGF‐β receptors by retarding proteasomal degradation of TGF‐β RI. This study clarifies the mechanism underlying hyperosmotic‐induced renal fibrosis in renal distal tubule cells. J. Cell. Biochem. 109: 663–671, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
148.
149.
150.