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排序方式: 共有196条查询结果,搜索用时 31 毫秒
51.
Kohichi Ohshima Masahiro Kikuchi Yoshiaki Sumiyoshi Yuhichi Masuda Hidehito Mohtai Fuyuki Eguchi Morishige Takeshita 《Virchows Archiv. B, Cell pathology including molecular pathology》1992,61(1):97-100
The phenotypes of proliferating cells in histiocytic necrotizing lymphadenitis (HNL) were examined. The affected areas consisted
mainly of CD 8-positive (suppressor/cytotoxic T-cells) and CD 4-positive (helper/inducer T-cells) in association with some
CD 15-positive cells (monocytes). A marker of proliferating cells (Ki-67) and monoclonal antibodies for determining the phenotypes
of cells (CD 4, CD 8, CD 15) in the affected areas were applied using a double-staining method. Ki-67-positive proliferating
cells were mainly CD 8-positive. A few CD 4-positive cells and rare CD 15-positive cells were also Ki-67-positive. The percentage
of CD 8-positive cells increased gradually over time and the ratio of CD 8-positive to proliferating cells did not decrease
throughout the observation period of 6 weeks. These results suggest that the proliferation of CD 8-positive T-cells together
with the accumulation of CD 4- and CD 15-positive cells is the main phenomenon occurring in HNL. 相似文献
52.
Yujiro Asada Seiichiro Hara Kousuke Marutsuka Kazuo Kitamura Tetsuo Tsuji Junichiro Sakata Yuichiro Sato Atsushi Kisanuki Tanenao Eto A. Sumiyoshi 《Histochemistry and cell biology》1999,112(3):185-191
Adrenomedullin (AM) is a novel hypotensive and vasodilator peptide. We previously examined the localization of AM in human,
rat, and porcine tissues using a polyclonal antibody against synthetic human AM[40–52]. We demonstrated that AM is widely
distributed in the endocrine and neuroendocrine systems, but not in the heart, kidney, or blood vessels, although high levels
of AM mRNA were detected in the latter tissues. In this study, we further investigated the distribution of AM by using two
newly developed monoclonal antibodies against synthetic human AM peptides, [12–25] and [46–52]. AM immunoreactivity was observed
in cardiac myocytes, vascular smooth muscle cells, endothelial cells, and renal distal and collecting tubules. In addition,
AM-immunoreactive (IR) cells were found in mucosal and glandular epithelia of the digestive, respiratory, and reproductive
systems, as well as the endocrine and neuroendocrine systems. These findings indicate that AM-IR cells are more widely distributed
in human tissues and suggest that AM might play multiple biological roles in humans.
Accepted: 7 June 1999 相似文献
53.
Shin T Sumiyoshi H Matsuo N Satoh F Nomura Y Mimata H Yoshioka H 《Archives of biochemistry and biophysics》2005,435(2):291-302
The serum level of prostate-specific antigen (PSA) is useful as a clinical marker for diagnosis and assessment of the progression of prostate cancer, and in evaluating the effectiveness of treatment. We characterized four Sp1/Sp3 binding sites in the proximal promoter of the PSA gene. In a luciferase assay, these sites contributed to the basal promoter activity in prostate cancer cells. In an electrophoretic mobility shift assay and chromatin immunoprecipitation assay, we confirmed that Sp1 and Sp3 bind to these sites. Overexpression of wild-type Sp1 and Sp3 further upregulated the promoter activity, whereas overexpression of the Sp1 dominant-negative form or addition of mithramycin A significantly reduced the promoter activity and the endogenous mRNA level of PSA. Among the four binding sites, a GC box located at nucleotides -53 to -48 was especially critical for basal promoter activity. These results indicate that Sp1 and Sp3 are involved in the basal expression of PSA in prostate cancer cells. 相似文献
54.
Morimatsu M Nakamura A Sumiyoshi H Sakaba N Taniguchi H Kohama K Higashi-Fujime S 《Biochemical and biophysical research communications》2000,270(1):147-152
Chara myosin in green algae, Chara corallina, is the fastest myosin of all those observed so far. To shed light on the molecular mechanism of this fast sliding, we determined the primary structure of Chara myosin heavy chain (hc). It has a motor domain, six IQ motifs for calmodulin binding, a coiled-coil structure to dimerize, and a globular tail. Chara myosin hc is very similar to some plant myosins and has been predicted to belong to the class XI. Short loop 1 and loop 2 may account for the characteristics of mechanochemical properties of Chara myosin. 相似文献
55.
The hatching time of Locusta migratoria under outdoor conditions: role of temperature and adaptive significance
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The present study investigates the time of hatching of the migratory locust Locusta migratoria using egg pods that are artificially buried in the soil under outdoor conditions. Most eggs hatch in the mid‐morning, with a peak between 11.00 and 12.00 h, and none hatch before 09.00 or after 16.00 h. Furthermore, most egg pods complete hatching within a day, although some take 2 or 3 days, and egg hatching is interrupted by rain. There are no large differences in hatching time from May to September. Laboratory experiments in which the eggs are exposed to temperatures simulating outdoor conditions show that soil temperature is the main factor controlling hatching activity. The increase in temperature in the morning appears to trigger egg hatching, as confirmed by laboratory experiments, which may explain the similar hatching times between seasons. The seasonal patterns of temperature variation and hatching time suggest that the hatching time of L. migratoria eggs may be adjusted to allow the hatchlings to be exposed to high temperatures in the afternoon so that they can harden their bodies quickly. 相似文献
56.
57.
To investigate the relationship between phylogeny and glycan structures, we analyzed the structure of planarian N-glycans. The planarian Dugesia japonica, a member of the flatworm family, is a lower metazoan. N-glycans were prepared from whole worms by hydrazinolysis, followed by tagging with the fluorophore 2-aminopyridine at their reducing end. The labeled N-glycans were purified, and separated by three HPLC steps. By comparison with standard pyridylaminated N-glycans, it was shown that the N-glycans of planarian include high mannose-type and pauci-mannose-type glycans. However, many of the major N-glycans from planarians have novel structures, as their elution positions did not match those of the standard glycans. The results of mass spectrometry and sugar component analyses indicated that these glycans include methyl mannoses, and that the most probable linkage was 3-O-methylation. Furthermore, the methyl residues on the most abundant glycan may be attached to the non-reducing-end mannose, as the glycans were resistant to α-mannosidase digestion. These results indicate that methylated high-mannose-type glycans are the most abundant structure in planarians. 相似文献
58.
Yoshida T Suga M Arima K Muranaka Y Tanaka T Eguchi S Lin C Yoshida S Ishikawa M Higuchi Y Seo T Ueoka Y Tomotake M Kaneda Y Darby D Maruff P Iyo M Kasai K Higuchi T Sumiyoshi T Ohmori T Takahashi K Hashimoto K 《PloS one》2011,6(5):e20469
Background
The CogState Schizophrenia Battery (CSB), a computerized cognitive battery, covers all the same cognitive domains as the Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) Consensus Cognitive Battery but is briefer to conduct. The aim of the present study was to evaluate the criterion and construct validity of the Japanese language version of the CSB (CSB-J) in Japanese patients with schizophrenia.Methodology/Principal Findings
Forty Japanese patients with schizophrenia and 40 Japanese healthy controls with matching age, gender, and premorbid intelligence quotient were enrolled. The CSB-J and the Brief Assessment of Cognition in Schizophrenia, Japanese-language version (BACS-J) were performed once. The structure of the CSB-J was also evaluated by a factor analysis. Similar to the BACS-J, the CSB-J was sensitive to cognitive impairment in Japanese patients with schizophrenia. Furthermore, there was a significant positive correlation between the CSB-J composite score and the BACS-J composite score. A factor analysis showed a three-factor model consisting of memory, speed, and social cognition factors.Conclusions/Significance
This study suggests that the CSB-J is a useful and rapid automatically administered computerized battery for assessing broad cognitive domains in Japanese patients with schizophrenia. 相似文献59.
Kumagai A Horike N Satoh Y Uebi T Sasaki T Itoh Y Hirata Y Uchio-Yamada K Kitagawa K Uesato S Kawahara H Takemori H Nagaoka Y 《PloS one》2011,6(10):e26148
Flavonoids, which are plant polyphenols, are now widely used in supplements and cosmetics. Here, we report that 4'-methylflavonoids are potent inducers of melanogenesis in B16F10 melanoma cells and in mice. We recently identified salt inducible kinase 2 (SIK2) as an inhibitor of melanogenesis via the suppression of the cAMP-response element binding protein (CREB)-specific coactivator 1 (TORC1). Using an in vitro kinase assay targeting SIK2, we identified fisetin as a candidate inhibitor, possibly being capable of promoting melanogenesis. However, fisetin neither inhibited the CREB-inhibitory activity of SIK2 nor promoted melanogenesis in B16F10 melanoma cells. Conversely, mono-methyl-flavonoids, such as diosmetin (4'-O-metlylluteolin), efficiently inhibited SIK2 and promoted melanogenesis in this cell line. The cAMP-CREB system is impaired in A(y)/a mice and these mice have yellow hair as a result of pheomelanogenesis, while Sik2(+/-); A(y)/a mice also have yellow hair, but activate eumelanogenesis when they are exposed to CREB stimulators. Feeding Sik2(+/-); A(y)/a mice with diets supplemented with fisetin resulted in their hair color changing to brown, and metabolite analysis suggested the presence of mono-methylfisetin in their feces. Thus, we decided to synthesize 4'-O-methylfisetin (4'MF) and found that 4'MF strongly induced melanogenesis in B16F10 melanoma cells, which was accompanied by the nuclear translocation of TORC1, and the 4'-O-methylfisetin-induced melanogenic programs were inhibited by the overexpression of dominant negative TORC1. In conclusion, compounds that modulate SIK2 cascades are helpful to regulate melanogenesis via TORC1 without affecting cAMP levels, and the combined analysis of Sik2(+/-) mice and metabolites from these mice is an effective strategy to identify beneficial compounds to regulate CREB activity in vivo. 相似文献
60.
Yudai Nishide Kenta Watanabe Hiroki Inoue Hiromitsu Moriyama Toshiyuki Satoh Norihide Hinomoto Kikuo Iwabuchi 《Applied Entomology and Zoology》2013,48(1):93-96
We developed and characterized nine microsatellite loci for the polyembryonic wasp Copidosoma floridanum (Ashmead) (Hymenoptera: Encyrtidae). Eight of the nine loci were polymorphic and did not deviate from Hardy–Weinberg equilibrium. Our results will contribute to studies on the population genetic structure of C. floridanum. 相似文献