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51.
目的:探究椎间孔镜与开窗术对腰椎间盘突出患者治疗远期效果对比。方法:选择2016年3月至2018年3月于我院接受治疗的腰椎间盘突出患者,按照其接受术式的不同将其分为孔镜组(108例)和开窗组(40例),对比两组手术出血量、术后卧床时间及切口长度,对比两组术前、术后3个月及术后12个月腰椎日本矫形外科学会(Japan Orthopaedic Assoctiation,JOA)评分、Odwestry功能障碍指数(Odwestris ability index, ODI)评分、视觉模拟评分(Visual analog scales,VAS)及生活质量评分,最后对比两组术后12个月椎间隙高度降低数值。结果:(1)孔镜组术中出血量、术后卧床时间及切口长度均小于开窗组,手术时间长于开窗组(P0.05);(2)术前两者JOA及ODI评分对比无统计学意义(P0.05),术后3个月及术后12个月孔镜组JOA及ODI评分优于开窗组(P0.05);(3)术前两组VAS及SF-36量表(the 36-item shot form health survey,SF-36)评分对比无统计学意义(P0.05),术后3个月及12个月两组VAS评分均有明显下降,SF-36评分有明显上升(P0.05),且术后3个月及12个月孔镜组SF-36评分高于开窗组(P0.05),VAS评分对比无统计学意义(P0.05);(4)术后12个月,孔镜组椎间隙高度降低率低于开窗组(P0.05)。结论:椎间孔镜在治疗腰椎间盘突出方面效果较好,相比于开窗术,孔镜术患者术中创伤小、术后恢复快、腰椎功能改善明显,且远期随访显示患者生活质量更高,值得进行临床推广。 相似文献
52.
Wu Hao Cui Yuanting He Chengkang Gao Peng Li Qiang Zhang Hexuan Jiang Yanli Hu Yingru Wei Xiao Lu Zongshi Ma Tianyi Liu Daoyan Zhu Zhiming 《中国科学:生命科学英文版》2020,63(11):1665-1677
Science China Life Sciences - High salt intake is a known risk factor of cardiovascular diseases. Our recent study demonstrated that long-term high salt intake impairs transient receptor potential... 相似文献
53.
The Chinese black truffle Tuber indicum is commercially valuable. The main factors influencing the success or failure of a truffle crop include the mycorrhizal colonization rate and host plant quality. The effects of a plant growth regulator (uniconazole) and plant growth management technique (root-tip cutting) on T. indicum colonization rate and Pinus armandii seedling growth were assessed under greenhouse conditions. The results indicated that 10 mg l−1 uniconazole or the combination of 5 mg l−1 uniconazole and root-tip cutting constitutes an effective method for ectomycorrhizal synthesis based on an overall evaluation of colonization rate, plant biomass, plant height, root weight, stem circumference and antioxidant enzyme activities (SOD and POD) of P. armandii. The abundance of Proteobacteria in the rhizosphere of colonized seedlings might serve as an indicator of stable mycorrhizal colonization. This research inspires the potential application of uniconazole and root-tip cutting treatments for mycorrhizal synthesis and truffle cultivation. 相似文献
54.
Hui Yang Philippe Ciais Maurizio Santoro Yuanyuan Huang Wei Li Yilong Wang Ana Bastos Daniel Goll Almut Arneth Peter Anthoni Vivek K. Arora Pierre Friedlingstein Vanessa Harverd Emilie Joetzjer Markus Kautz Sebastian Lienert Julia E. M. S. Nabel Michael O'Sullivan Stephen Sitch Nicolas Vuichard Andy Wiltshire Dan Zhu 《Global Change Biology》2020,26(7):3997-4012
Gaps in our current understanding and quantification of biomass carbon stocks, particularly in tropics, lead to large uncertainty in future projections of the terrestrial carbon balance. We use the recently published GlobBiomass data set of forest above‐ground biomass (AGB) density for the year 2010, obtained from multiple remote sensing and in situ observations at 100 m spatial resolution to evaluate AGB estimated by nine dynamic global vegetation models (DGVMs). The global total forest AGB of the nine DGVMs is 365 ± 66 Pg C, the spread corresponding to the standard deviation between models, compared to 275 Pg C with an uncertainty of ~13.5% from GlobBiomass. Model‐data discrepancy in total forest AGB can be attributed to their discrepancies in the AGB density and/or forest area. While DGVMs represent the global spatial gradients of AGB density reasonably well, they only have modest ability to reproduce the regional spatial gradients of AGB density at scales below 1000 km. The 95th percentile of AGB density (AGB95) in tropics can be considered as the potential maximum of AGB density which can be reached for a given annual precipitation. GlobBiomass data show local deficits of AGB density compared to the AGB95, particularly in transitional and/or wet regions in tropics. We hypothesize that local human disturbances cause more AGB density deficits from GlobBiomass than from DGVMs, which rarely represent human disturbances. We then analyse empirical relationships between AGB density deficits and forest cover changes, population density, burned areas and livestock density. Regression analysis indicated that more than 40% of the spatial variance of AGB density deficits in South America and Africa can be explained; in Southeast Asia, these factors explain only ~25%. This result suggests TRENDY v6 DGVMs tend to underestimate biomass loss from diverse and widespread anthropogenic disturbances, and as a result overestimate turnover time in AGB. 相似文献
55.
Yingfan Cai Xiaoyan Cai Qinglian Wang Ping Wang Yu Zhang Chaowei Cai Yanchao Xu Kunbo Wang Zhongli Zhou Chenxiao Wang Shuaipeng Geng Bo Li Qi Dong Yuqing Hou Heng Wang Peng Ai Zhen Liu Feifei Yi Minshan Sun Guoyong An Jieru Cheng Yuanyuan Zhang Qian Shi Yuanhui Xie Xinying Shi Ying Chang Feifei Huang Yun Chen Shimiao Hong Lingyu Mi Quan Sun Lin Zhang Baoliang Zhou Renhai Peng Xiao Zhang Fang Liu 《Plant biotechnology journal》2020,18(3):814-828
56.
Qiang Zhang Weiwei Li Jiapeng Yang Junjie Xu Yuling Meng Weixing Shan 《Molecular Plant Pathology》2020,21(4):541-554
Proteases secreted by pathogens have been shown to be important virulence factors modifying plant immunity, and cysteine proteases have been demonstrated to participate in different pathosystems. However, the virulence functions of the cysteine proteases secreted by Phytophthora parasitica are poorly understood. Using a publicly available genome database, we identified 80 cysteine proteases in P. parasitica, 21 of which were shown to be secreted. Most of the secreted cysteine proteases are conserved among different P. parasitica strains and are induced during infection. The secreted cysteine protease proteins PpCys44/45 (proteases with identical protein sequences) and PpCys69 triggered cell death on the leaves of different Nicotiana spp. A truncated mutant of PpCys44/45 lacking a signal peptide failed to trigger cell death, suggesting that PpCys44/45 functions in the apoplastic space. Analysis of three catalytic site mutants showed that the enzyme activity of PpCys44/45 is required for its ability to trigger cell death. A virus-induced gene silencing assay showed that PpCys44/45 does not induce cell death on NPK1 (Nicotiana Protein Kinase 1)-silenced Nicotiana benthamiana plants, indicating that the cell death phenotype triggered by PpCys44/45 is dependent on NPK1. PpCys44- and PpCys45-deficient double mutants showed decreased virulence, suggesting that PpCys44 and PpCys45 positively promote pathogen virulence during infection. PpCys44 and PpCys45 are important virulence factors of P. parasitica and trigger NPK1-dependent cell death in various Nicotiana spp. 相似文献
57.
Jonathan Hurtado Dhiraj Acharya Huafang Lai Haiyan Sun Somanath Kallolimath Herta Steinkellner Fengwei Bai Qiang Chen 《Plant biotechnology journal》2020,18(1):266-273
Chikungunya virus (CHIKV) is a mosquito‐transmitted alphavirus, and its infection can cause long‐term debilitating arthritis in humans. Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections. In this study, we explored the feasibility of using an anti‐CHIKV monoclonal antibody (mAb) produced in wild‐type (WT) and glycoengineered (?XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model. CHIKV mAb was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme. While mAb produced in ?XFT carried a single N‐glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N‐glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures. Both WT and ?XFT plant‐produced mAbs demonstrated potent in vitro neutralization activity against CHIKV. Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ?XFT‐produced mAbs. This is the first report of the efficacy of plant‐produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation. 相似文献
58.
59.
60.
Luming Zhuang Fei Peng Yuanyuan Huang Wenzhe Li Jiuhong Huang Yunqiang Chu Pu Ren Ying Sun Yan Zhang Elleen Xue Xiaowei Guo Xiafeng Shen Lei Xue 《Aging cell》2020,19(2)
Dysregulation of autophagy is associated with the neurodegenerative processes in Alzheimer's disease (AD), yet it remains controversial whether autophagy is a cause or consequence of AD. We have previously expressed the full‐length human APP in Drosophila and established a fly AD model that exhibits multiple AD‐like symptoms. Here we report that depletion of CHIP effectively palliated APP‐induced pathological symptoms, including morphological, behavioral, and cognitive defects. Mechanistically, CHIP is required for APP‐induced autophagy dysfunction, which promotes Aβ production via increased expression of BACE and Psn. Our findings suggest that aberrant autophagy is not only a consequence of abnormal APP activity, but also contributes to dysregulated APP metabolism and subsequent AD pathogenesis. 相似文献