城市生态园林与生物多样性保护

城市生态园林与生物多样性保护袁兴中,刘红（曲阜师范大学,２７３１６５）ＵｒｂａｎＥｃｏｌｏｇｉｃａｌｇａｒｄｅｎｉｎｇａｎｄＢｉｏｄｉｖｅｒｓｉｔｙＣｏｎｓｅｒｖａｔｉｏｎ．￥ＹｕａｎＸｉｎｇｚｈｏｎｇ;ＬｉｕＨｏｎｇ（Ｄｅ－ｐａｒｔｍｅｎｔｏｆＢｉ...     

新疆的刺球座属、穴壳属及普氏腔孢属

本文报道了新疆腔菌纲座囊菌目刺球座属(Lasiobotrys)、穴壳属(Dothiora)和普氏腔孢属(Plowrightia)的六种子囊菌,即:忍冬刺球座菌(L.loniccrae)、花楸穴壳菌 (D.sorbi)及其无性阶段花楸疡壳孢(Dothichiza sorbi)、茶蔗子普氏腔孢菌(P.ribesia)、小檗普氏腔孢菌(P.berberidiJ)、沙棘普氏腔孢菌(P.hippophaeos)及雕刻普氏腔孢菌(P.insculpta)。这三个属的真菌在我国均未报道过,为我国新纪录属(种)。标本均采于新疆,保存于新疆八一农学院植保系真菌标本室(HMAAC)。     

九株根霉可溶性蛋白和三种酶的电泳图谱比较研究

本文报导了根霉属(Rhizopus) 9个菌株天然态及解聚态可溶性蛋白、酯酶同工酶、葡萄糖淀粉酶和SOD电泳图谱的比较研究。结果表明：可溶性蛋白图谱和酯酶同工酶谱能显示五种已知供试菌种间的差异,尤其酯酶同工酶谱还能显示米根霉两个供试菌株之间的微小差异。经综合分析全部试验结果后得出的系统树图显示了9个供试菌株间的亲缘关系,并为未知菌株F1(BR12)和Q303提供了鉴定和命名依据。文中首次报导了根霉的SOD同工酶,并对蛋白质和酶电泳图谱用于根霉分类研究进行了讨论。     

A phenotypic host range alteration determines RD114 virus restriction in feline embryonic cells.

We have characterized the restriction mechanism for RD114 virus replication in embryonic feline cells (FeF). By comparing growth properties of the virus in FeF cells with its behavior in a fetal feline glial cell line (G355) permissive for RD114, we showed that both cell lines were readily infectible by virus grown in permissive cells and that no significant differences in viral integration or viral RNA expression could be detected. However, analysis of viral protein expression revealed differences in viral env gene processing in the two cell types. Envelope precursor pR85 was produced, but the expected processed gp70 product was detectable only in permissive (G355) cells. An envelope product of 85 kDa was packaged into virions produced by FeF cells, while virions produced by G355 cells contained the expected RD114 gp70. While the gp85 env-containing virions were infectious for permissive G355 cells, they were unable to infect FeF cells. The block to infection by the gp85-containing particles in FeF cells could be abrogated by treatment with the glycosylation inhibitor tunicamycin. Our results indicate that restriction of RD114 virus involves a novel mechanism dependent on two factors: altered glycosylation of the envelope to a gp85 form and an altered RD114 receptor in FeF cells.     

轮状病毒新敏感细胞的筛选

轮状病毒（RV）的细胞培养问题的尚未完全解决,其原因之一可能与敏感细胞较少有关。为此,我们进行了RV新敏感细胞的筛选,发现恒河猴胚肾传代细胞Frhk-4感染不同血清型的人和动物RV标准株Wa、DS－1、YO、ST－3、SA－11和UK,37℃旋转或静止培养均能产生明显CPE。培养物经ELISA测定含RV抗原,用PAGE检测有特征性RV核酸图型,电镜超萍切片检查感染细胞可见RV颗粒,证实Rrhk-4是RV的敏感细胞,这在国内外尚未见报道。同时与MA104－CV－1细胞的比较研究证明,三种细胞对Wa和DS－1的敏感性及增殖滴度,以CV－1最高,Frhk-4次之,MA－104最低。     

骨缘当归的化学成分

骨缘当归Angellica cartilaginomarginata var.foliata Yuan et Shen,别名:山藁本、野芹菜（江苏）,仅分布于江苏和浙江。在江苏镇江等地用干燥全草作山藁本入药,其化学成分未见报道。 样品采自江苏省句容县。将干根磨粉,提取物经中性氧化铝层析,根据色谱及光谱鉴定为两种脂肪酸、四种已知香豆素和一种植物甾醇即:正葵酸（n-capric acid）、月桂酸（lauric acid）、骨缘当归素（cartilaginomarginadin）、蝉翼素（pteryxin）、白花前胡素F（praeruptorin F）、佛手柑内酯（bergapten）及β-谷甾醇（β-sitosterol）。     

卡介苗对枯否细胞生物特性影响的免疫细胞化学研究

卡介苗(BCG)是增强肝枯否细胞(KC)抗肝癌细胞作用的最佳免疫制剂之一。本实验是给大鼠BCG后,应用溶菌酶免疫细胞化学法显示KC,以图象分析系统测定KC的数量、分布和面积,以细胞分光光度计测定KC溶菌酶活性;分离KC体外受BCG作用后,用ELISA法测定RC的Fc受体表达。结果显示:BCG使KC数量增多一倍,尤以肝小叶周边带为显著;KC体积明显增大,细胞面积增大55.71％,周边带KC面积增大一倍上;细胞溶菌酶光密度增大64.35％;Fc受体表达增强64.82％。上述结果是BCG增强KC抗肝癌细胞作用有关细胞学机制。