微循环研究常用部位的肽能和胺能神经支配

以逆行追踪与免疫细胞化学相结合法,探讨了大鼠提睾肌、盲肠系膜和耳廓等微循环研究常用部位的神经肽Ｙ（ＮＰＹ）、降钙素基因相关肽（ＣＧＲＰ）、甲啡吠（Ｍ－ＥＮＤ）和５－羟色胺（５－ＨＴ）等肽能和胺能神经的支配。结果表明：支配提睾肌的运动和感觉神经元分别含有５－ＨＴ和ＣＧＲＰ。支配提睾肌血管、盲肠系膜及其血管的交感神经元,一部分含有ＮＰＹ,一部分含有Ｍ－ＥＮＫ;支配耳廓局部的运动和感觉神经元均含有ＣＧＲ     

中国蚋属一新种（双翅目：蚋科）

本文报道中国蚋属绳蚋亚属一新种－重庆绳蚋,新种Ｓ．（Ｇ．）ｃｈｏｎｇｑｉｎｇｅｎｓｉｓｓｐ．ｎｏｖ．模式标本采自重庆四面山,保存于重庆市卫生防疫站。     

Optimization of the culture medium composition for the antibody response of mouse spleen cells

Summary The Mishell-Dutton culture system for in vitro primary antibody response of mouse spleen cells was used to optimize the amino acid composition of RPMI 1640 media. Each of the 20 amino acids was tested over a broad range of concentrations always leaving the remaining 19 amino acids unaltered (i.e. at the formula recommended concentration). In several instances, higher plaque-forming cell responses were obtained with an amino acid concentration that was either higher or lower than that recommended: (a) the optimum concentration for valine, glutamine, and lysine lies considerably above the recommended one, (b) the optimum concentration for leucine as well as for several other amino acids lies below the recommended concentration, and (c) the optimum concentration for arginine corresponds exactly to the recommended concentration. The second round of optimization, i.e. combining of two conditions that individually yielded an improved response often caused a decrease of response. The possibility is discussed that for an optimal response a ratio of two or several amino acids rather than the absolute concentration of any one amino acid is of importance. The Basel Institute for Immunology was founded and is supported by F. Hoffman-La Roche & Co., Ltd.     

Mechanisms of increasing the intensity of ultraviolet fluorescence of irradiated cells

As shown on the in vitro irradiated Ehrlich ascites tumor cells the increase in the intensity of ultraviolet fluorescence (UVF) of the exposed cells occurs only in such incubation conditions when an increase in the tryptophan content of irradiated cells becomes possible. The increase in the UVF intensity and in the tryptophan content are quantitatively identical. It is concluded that the increase in the content of the fluorescing substance, tryptophan, in the irradiated cells is the cause of the increase in the UVF intensity.     

Nicotiana chloroplast genome

Summary RuBPCase, the enzyme responsible for carboxylation and oxidation of RuBP in a wide variety of photosynthetic organisms, is the major protein found in the chloroplast. Here we present the first evidence for direct expression in E. coli and B. subtilis of tobacco and Chlamydomonas ct-DNA sequences coding for the LS of RuBPCase as demonstrated by a simple in situ immunoassay.     

Light-induced transformation of amyloplasts into chloroplasts in potato tubers

The transformation of amyloplast into chloroplasts in potato (Solanum tuberosum L.) tuber tissue can be induced by light. Excised potato tuber discs illuminated with white light of 3000 lux began to synthesize chlorophyll after a lag period of 1 day, and continued to synthesize chlorophyll for 3 weeks. In this paper we present evidence, based on ultracentrifugal sedimentation and immunoprecipitation, that the light-mediated synthesis of Ribulose-1,5-bisphosphate carboxylase began 1 day after illumination with white light. When illuminated the chloroplasts isolated from light-grown potato tuber tissue incorporated [³⁵S]methionine into polypeptides, one of which has been identified as the large subunit of Ribulose-1,5-bisphosphate carboxylase. These chloroplasts are functional as determined by O₂ evolution in the Hill reaction.     

花叶芋的组织培养和植株再生

双色花叶芋(Caladium bieolor)和亮白花叶芋(C.hortulanum)的叶及花序外植体在加有2,4-D 和激动素或只加有2,4-D 的培养基上产生了愈伤组织,它们在转移到无激素或含激动素和低浓度生长素的培养基上以后分化出大量胚状体,并进一步长成小植株。本工作为花叶芋的快速繁殖提供了方法。     

应用群分析研究薯蓣的分类

本文是应用群分析研究薯蓣属根状茎组中国分类群分类的一次尝试。分类特征取用了形态学、细胞学、花粉形态和生物化学等多方面的性状进行综合分析;方法和手段上使用了距离系数、相关系数等各种群分析运算方法,并且以综合系数进行评价。不仅取得了与传统分类基本一致的分类结果,为该组植物的系统分类提供富有启发性的参考,同时也为高等植物数量分类研究找到一种最优分类方法一相关系数UPGMA法。     

MIXED MODEL APPROACHES FOR ESTIMATING GENETIC VARIANCES AND COVARIANCES

The limitations of methods for analysis of variance(ANOVA)in estimating genetic variances are discussed. Among the three methods(maximum likelihood ML, restricted maximum likelihood REML, and minimum norm quadratic unbiased estimation MINQUE)for mixed linear models, MINQUE method is presented with formulae for estimating variance components and covariances components and for predicting genetic effects. Several genetic models, which cannot be appropriately analyzed by ANOVA methods, are introduced in forms of mixed linear models. Genetic models with independent random effects can be analyzed by MINQUE(1)method whieh is a MINQUE method with all prior values setting 1. MINQUE(1)method can give unbiased estimation for variance components and covariance components, and linear unbiased prediction (LUP) for genetic effects. There are more complicate genetic models for plant seeds which involve correlated random effects. MINQUE(0/1)method, which is a MINQUE method with all prior covariances setting 0 and all prior variances setting 1, is suitable for estimating variance and covariance components in these models. Mixed model approaches have advantage over ANOVA methods for the capacity of analyzing unbalanced data and complicated models. Some problems about estimation and hypothesis test by MINQUE method are discussed.