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971.
Shuting Ku Ying Wang Nan Yang 《Biochemical and biophysical research communications》2010,394(4):871-4991
PEGylated PAMAM conjugated fluorescein-doped magnetic silica nanoparticles (PEGylated PFMSNs) have been synthesized for evaluating their ability across the blood-brain barrier (BBB) and distribution in rat brain. The obtained nanoparticles were characterized by transmission electron microscopy (TEM), thermal gravimetry analyses (TGA), zeta potential (ζ-potential) titration, and X-ray photoelectron spectroscopy (XPS). The BBB penetration and distribution of PEGylated PFMSNs and FMSNs in rat brain were investigated not only at the cellular level with Confocal laser scanning microscopy (CLSM), but also at the subcellular level with transmission electron microscopy (TEM). The results provide direct evidents that PEGylated PFMSNs could penetrate the BBB and spread into the brain parenchyma. 相似文献
972.
Tiit Nikopensius Kaarel Krjutškov Veronika Tammekivi Mare Saag Inga Prane Linda Piekuse Ilze Akota Biruta Barkane Astrida Krumina Laima Ambrozaitytė Aušra Matulevičienė Zita Aušrelė Kučinskienė Baiba Lace Vaidutis Kučinskas Andres Metspalu 《Birth defects research. Part A, Clinical and molecular teratology》2010,88(9):748-756
973.
Izabela Nowak Edyta Majorczyk Andrzej Wi?niewski Andrzej Pawlik Maria Magott-Procelewska Ewa Passowicz-Muszyńska Jacek Malejczyk Rafa? P?oski Sebastian Giebel Ewa Barcz Aleksandra Zoń-Giebel Andrzej Malinowski Henryk Tchórzewski Arkadiusz Chlebicki Wioleta ?uszczek Maciej Kurpisz Marian Grybo? Jacek Wilczyński Piotr Wiland David Senitzer Ji-Yao Sun Renata Jankowska Marian Klinger Piotr Ku?nierczyk 《PloS one》2010,5(8)
Background
KIR2DS5 gene encodes an activating natural killer cell receptor whose ligand is not known. It was recently reported to affect the outcome of hematopoietic stem cell transplantation.Methodology/Principal Findings
In our studies on KIR2DS5 gene associations with human diseases, we compared the frequencies of this gene in patients and relevant controls. Typing for KIR2DS5 gene was performed by either individual or multiplex polymerase chain reactions which, when compared in the same samples, gave concordant results. We noted an apparently protective effect of KIR2DS5 gene presence in several clinical conditions, but not in others. Namely, this effect was observed in ankylosing spondylitis (p = 0.003, odds ratio [OR] = 0.47, confidence interval [CI] = 0.28–0.79), endometriosis (p = 0.03, OR = 0.25, CI = 0.07–0.82) and acute rejection of kidney graft (p = 0.0056, OR = 0.44, CI = 0.24–0.80), but not in non-small-cell lung carcinoma, rheumatoid arthritis, spontaneous abortion, or leukemia (all p>0.05). In addition, the simultaneous presence of KIR2DS5 gene and HLA-C C1 allotype exhibited an even stronger protective effect on ankylosing spondylitis (p = 0.0003, OR = 0.35, CI = 0.19–0.65), whereas a lack of KIR2DS5 and the presence of the HLA-C C2 allotype was associated with ankylosing spondylitis (p = 0.0017, OR = 1.92, CI = 1.28–2.89), whereas a lack of KIR2DS5 and presence of C1 allotype was associated with rheumatoid arthritis (p = 0.005, OR = 1.47, CI = 1.13–1.92). The presence of both KIR2DS5 and C1 seemed to protect from acute kidney graft rejection (p = 0.017, OR = 0.47, CI = 0.25–0.89), whereas lack of KIR2DS5 and presence of C2 seemed to favor rejection (p = 0.0015, OR = 2.13, CI = 1.34–3.37).Conclusions/Significance
Our results suggest that KIR2DS5 may protect from endometriosis, ankylosing spondylitis, and acute rejection of kidney graft. 相似文献974.
RSSG58基因在水稻精细胞中的表达 总被引:1,自引:0,他引:1
RSSG5 8是利用抑制差减杂交技术从水稻精细胞文库中筛选到的在精细胞中优势表达的基因 ,推测其编码的蛋白质与拟南芥的肌球重链蛋白有一定的同源性 (4 6 % ) ,并具有肌球蛋白特色的结构域。把RSSG5 8基因开放编码框连接到表达载体pQE30上 ,重组质粒在E .coliM15中表达出N端融合了 6×His的融合蛋白。SDS PAGE分析表明 ,表达产物的分子量约为 6 6kD ,其表达量占菌体总蛋白的 8.6 %。分离纯化融合蛋白来免疫家兔 ,制得了高效价、高特异性的多克隆抗体。Western杂交显示 ,在分离的精细胞内该基因编码的蛋白表达量很高 ,而成熟花粉和二细胞中只有微弱表达 ,单细胞花粉、花粉母细胞没有杂交信号 ,表明RSSG5 8基因在精细胞中优势表达。 相似文献
975.
Hong Y Ku K Kim M Won M Chung K Song KB 《Journal of microbiology and biotechnology》2008,18(4):742-745
Inactivation of Escherichia coli O157:H7 and Salmonella typhimurium was evaluated on inoculated chicken by aqueous chlorine dioxide (ClO2) treatment. Chicken samples were inoculated with 6-7 log CFU/g of Escherichia coli O157:H7 and Salmonella typhimurium, respectively. The chicken samples were then treated with 0, 50, and 100 ppm of ClO2 solution and stored at 4 +/- 1 degrees C. Aqueous ClO2 treatment decreased the populations of the pathogenic bacteria on the chicken breast and drumstick. In particular, 100 ppm ClO2 treatment on the chicken breast and drumstick reduced Escherichia coli O157:H7 and Salmonella typhimurium by 1.00-1.27 and 1.37-1.44 log CFU/g, respectively. Aqueous ClO2 treatment on the growth of the bacteria was continuously in effect during storage, resulting in the decrease of the populations of Escherichia coli O157:H7 and Salmonella typhimurium. These results suggest that aqueous ClO2 treatment should be useful in improving the microbial safety of chicken during storage. 相似文献
976.
L Bicka J Ku?mak B Kozaczyńska A Plucienniczak A Skorupska 《Acta biochimica Polonica》2001,48(1):227-232
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle. 相似文献
977.
中国19个狂犬病病毒街毒分离株N基因的序列分析 总被引:35,自引:2,他引:33
测定了30年来从不同动物中分离的19个中国狂犬病病毒街毒株N基因的部分核酸序列,并对其核苷酸差异做了比较分析.可将中国狂犬病病毒街毒株分为4个组群,各组间的同源性为83.45%~88.62%.除广西地区分离的狂犬病病毒街毒株彼此差异较大外,其余街毒株的地理分布与其N基因核酸序列差异的距离是密切相关的,基本上可按其地理分布分为东、西二大组. 相似文献
978.
979.
980.
Development of a fluorescence stereomicroscope and observation of Bong-Han corpuscles inside blood vessels 总被引:1,自引:0,他引:1
Lee BC Yoo JS Baik KY Sung B Lee J Soh KS 《Indian journal of experimental biology》2008,46(5):330-335
A fluorescence stereomicroscope system was developed in order to observe in situ the distribution of nuclei in intravascular Bong-Han ducts and corpuscle tissues by injecting acridine orange, which stained specifically nuclei. Intravascular Bong-Han corpuscles, connected with Bong-Han ducts could be detected in the aortas of rats, mice, and rabbits. 相似文献