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71.
Acid lipase activity in the livers of thyroidectomized rats is increased by administration of L-thyroxine. The response is dose-dependent and can be demonstrated within 12 h after treatment. L-Triiodothyronine also evokes a rapid increase in acid lipase activity, and this increase can be inhibited by coadministration of actinomycin D. 相似文献
72.
Plasmids of Synechococcus cedrorum and two Anacytsis nidulans strains were characterized physically, and a probable instance of spontaneous "curing" is described. 相似文献
73.
The isolation and characterization of a specific antibody population directed against the prothrombin activation fragments F2 and F1 + 2 总被引:10,自引:0,他引:10
H K Lau J S Rosenberg D L Beeler R D Rosenberg 《The Journal of biological chemistry》1979,254(18):8751-8761
We have raised antisera against human prothrombin activation fragment F2 in rabbits and have chromatographed the respective immunoglobulin G fractions on prothrombin-Sepharose, Pr1-Sepharose, and F2-Sepharose immunoadsorbents. The specific antibody population obtained was utilized to construct a double antibody radioimmunoassay capable of measuring as little as 0.8 ng/ml of this component. Our studies suggest that the immunoreactive site defined by this antibody population is most probably located within the negatively charged COOH-terminal region of F2. The immunologic expression of this area is unaffected by denaturation or reduction-alkylation of F2 as well as by attachment of polypeptide to the NH2-terminal of this component. However, the presence of covalently bound polypeptide at the COOH-terminal of F2 reduces its immunologic reactivity by 300- to 400-fold. Prothrombin, Pr1, and Pr*1, which contain the F2 region as part of their covalent structure, are at least 4000 to 7000 times less immunoreactive than F2 on a molar basis. Conversion of these components to thrombin as well as activation fragments generates the theoretically predicted level of immunoreactivity. Masking of the immunoreactive site within these zymogens is due to two phenomena. Firstly, covalent attachment of polypeptides on the COOH-terminal of the F2 segment significantly depresses the reactivity of this region. Secondly, a critical S--S bridge aids in the sequenstration of the immunoreactive site. This cross-link may facilitate interactions between the COOH-terminal of the F2 segment and other regions of the zymogen. 相似文献
74.
A convenient and reliable method for simulatneous visualization of silver staining (Ag-NOR) of the nucleolus organizers and fluorescent bandings in metaphase chromosomes is described. Studies employing this combined procedure on human chromosomes revealed that the Ag-NOR patterns may be characteristic for each chromosome of each individual. 相似文献
75.
Summary Kadazans, the largest indigenous group in Sabah, northern Borneo, were surveyed for glyoxalase I, phosphoglucomutase I, red cell acid phosphatase, esterase D, adenosine deaminase, soluble glutamate pyruvate transaminase, soluble glutamate oxaloacetate transaminase, 6-phosphogluconate dehydrogenase, uridine monophosphate kinase, adenylate kinase, peptidase B and D, superoxide dismutase, C5, group specific component, haptoglobin and transferrin.Kadazans were found to be polymorphic for GLOI, PGMI, RCAP, esterase D, ADA, s-Gpt, 6PGD, UMPK, Gc, C5, haptoglobin and peptidase B. Rare variants were found for transferrin and peptidase D. No variant was found for s-Got, SOD and AK. 相似文献
76.
Theodore A. Slotkin Christopher Lau María Bartolomé Frederic J. Seidler 《Life sciences》1976,19(4):483-491
Incubation of isolated rat adrenomedullary storage vesicles with methadone produced inhibition of 3H-epinephrine uptake and promotion of release of endogenous catecholamines. Neither effect was seen using morphine, nor could morphine antagonize methadone-induced catecholamine release, suggesting that these actions are not mediated by opiate receptors. Inhibition of uptake by methadone appeared to contain a competitive component with a lower Ki for methadone compared to the Km for 3H-epinephrine. Despite competitive inhibition by methadone, the maximal uptake capacity (analogous to Vmax) as determined by double-reciprocal plots, was increased by the drug, probably as a result of greater availability of intravesicular storage sites because of the drug-induced of release endogenous catecholamines. Agents which enhance or block catecholamine transport into vehicles had no effect on the catecholamine release by methadone, indicating that the latter is separable from the action on uptake. These alterations of catecholamine uptake and release may play a role in the effects of methadone on the adrenal medulla . 相似文献
77.
The intact sacrospinalis (back) muscle of a rabbit was injected with [3H]ouabain and allowed to incubate for 20 min. The muscle was excised and homogenized and a microsomal preparation was made. This was placed on a sucrose density gradient and two protein bands were observed at 30 and 40% sucrose regions. Most of the [3H]ouabain was associated with the dense band. If the dense band was loaded with Ca oxalate and placed on a density gradient, then both protein and ouabain migrated equally to the bottom of the gradient. If the 40% band was treated with EDTA, both protein and ouabain migrated to 33% sucrose. It was therefore concluded that ouabain-binding vesicles were associated with the 40% band. If the 40% band was loaded with Ca oxalate and then passed through a French press and placed again on a gradient, the ouabain migrated to 22% sucrose while the protein bands appeared at 17 and 32% sucrose and in the pellet. It was concluded that ouabain-binding vesicles were distinct from the major vesicle population, but were mechanically linked until the linkage was destroyed by mechanical disruption. Electron microscopy revealed that the dense band of the gradient contained terminal cisternae and transverse tubules attached to the terminal cisternae in triad or diad junctions. It was concluded that the 40% band consisted mainly of terminal cisternae and that the ouabain-binding vesicles were transverse tubules attached to terminal cisternae. Transverse tubules therefore may be identified specifically after homogenization. 相似文献
78.
79.
In the female hamster, temporary sterility for a period of 10 or 15 days was induced by an intraperitoneal Silastic-PVP-tube containing 3.5 or 1.0 mg of PGF2alpha, respectively. All Silastic-PVP-PGF2alpha tube hearing animals regained fertility and delivered normal litters at various times after the placement of the tube. The release rate of 3-H-PGF2alpha from the Silastic-PVP tube was described and their potential use as a drug delivery system discussed. 相似文献
80.
In the female hamster, temporary sterility for a period of 10 or 15 days was induced by an intraperitoneal Silastic-PVP-tube containing 0.5 or 1.0 mg of PGF2α, respectively. All Silastic-PVP-PGF2α tube bearing animals regained fertility and delivered normal litters at various times after the placement of the tube. The release rate of 3H-PGF2α from the Silastic-PVP tube was described and their potential use as a drug delivery system discussed. 相似文献