Identification of MYH6 as the potential gene for human ischaemic cardiomyopathy

The present study aimed to explore the potential hub genes and pathways of ischaemic cardiomyopathy (ICM) and to investigate the possible associated mechanisms. Two microarray data sets ( GSE5406 and GSE57338 ) were downloaded from the Gene Expression Omnibus (GEO) database. The limma package was used to analyse the differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, Disease Ontology (DO) and Gene Ontology (GO) annotation analyses were performed. A protein-protein interaction (PPI) network was set up using Cytoscape software. Significant modules and hub genes were identified by the Molecular Complex Detection (MCODE) app. Then, further functional validation of hub genes in other microarrays and survival analysis were performed to judge the prognosis. A total of 1065 genes were matched, with an adjusted p < 0.05, and 17 were upregulated and 25 were downregulated with|log₂ (fold change)|≥1.2. After removing the lengthy entries, GO identified 12 items, and 8 pathways were enriched at adjusted p < 0.05 (false discovery rate, FDR set at <0.05). Three modules with a score >8 after MCODE analysis and MYH6 were ultimately identified. When validated in GSE23561 , MYH6 expression was lower in patients with CAD than in healthy controls (p < 0.05). GSE60993 data suggested that MYH6 expression was also lower in AMI patients (p < 0.05). In the GSE59867 data set, MYH6 expression was lower in CAD patients than in AMI patients and lower in heart failure (HF) patients than in non-HF patients. However, there was no difference at different periods within half a year, and HF was increased when MYH6 expression was low (p < 0.05–0.01). We performed an integrated analysis and validation and found that MYH6 expression was closely related to ICM and HF. However, whether this marker can be used as a predictor in blood samples needs further experimental verification.     

Parasites may exit immunocompromised northern pig-tailed macaques(Macaca leonina) infected with SIVmac239

Parasites can increase infection rates and pathogenicity in immunocompromised human immunodeficiency virus(HIV) patients. However, in vitro studies and epidemiological investigations also suggest that parasites might escape immunocompromised hosts during HIV infection.Due to the lack of direct evidence from animal experiments, the effects of parasitic infections on immunocompromised hosts remain unclear. Here,we detected 14 different parasites in six northern pig-tailed macaques(NPMs) before or at the 50 th week of simian immunodeficiency virus(SIV) infection by ELISA. The NPMs all carried parasites before viral injection. At the 50 th week after viral injection, the individuals with negative results in parasitic detection(i.e., 08247 and 08287) were characterized as the Parasites Exit(PE) group, with the other individuals(i.e., 09203, 09211, 10205, and 10225) characterized as the Parasites Remain(PR) group. Compared with the PR group, the NPMs in the PE group showed higher viral loads, lower CD4~+T cells counts, and lower CD4/CD8 rates. Additionally, the PE group had higher immune activation and immune exhaustion of both CD4~+and CD8~+T cells. Pathological observation showed greater injury to the liver, cecum, colon,spleen, and mesenteric lymph nodes in the PE group.This study showed more seriously compromised immunity in the PE group, strongly indicating that parasites might exit an immunocompromised host.     

中国颊脊隐翅虫属比桑颊脊隐翅虫群三新亚种(鞘翅目,隐翅虫科,隐翅虫亚科)

记述中国四川颊脊隐翅虫属Quedius比桑颊脊隐翅虫群(beesoni group) 3新亚种,北川颊脊隐翅虫Q. (M.)noboruitoi beichuanensis,片口颊脊隐翅虫Q. (M.)noboruitoi piankouatilis,二郎山颊脊隐翅虫Q.(M.)noboruitoi erlangshanus。首次记录来自中国重庆的比桑颊脊隐翅虫Q. (M.)beesoni Cameron。列出了比桑颊脊隐翅虫群中国已知种和亚种检索表。     

A new species of the genus Micropeplus Latreille(Coleoptera: Staphylinidae: Micropeplinae) from China

A new species of the genus Micropeplus Latreille is described under the name of M. songi sp. nov. Color photos of habitus, head, pronotum, elytra, wing, metasternum, the 3rd to 7th tergites of abdomen, the 8th sternite, and aedeagus are provided. This species was found on fungi from Mt. Wahui in Jiulong County, Sichuan, China.     

鲟源致病性豚鼠气单胞菌的分离及其生长特性

从患细菌性败血综合征的杂交鲟(Husohuso♀×Acipense rruthenus♂)肝和达氏鳇(H.dauricus)腹水中共分离出4株优势菌,经人工回归感染实验测定了其致病性,并通过ATB细菌鉴定仪对致病菌株进行了鉴定,此外,研究了致病菌株的生长特性。实验结果表明,菌株XL2-T对杂交鲟和达氏鳇具有致病性。经鉴定,菌株XL2-T为豚鼠气单胞菌(Aeromonas punctata caviae);其在无菌营养肉汤中摇床振荡培养时的生长曲线:0～1.5h为生长延迟期,1.5～28h为对数生长期,28～32h为稳定期,32h以后为衰亡期;其最适生长温度为25～30℃,最适生长pH为7,在NaCl含量范围0～10%、沙拉沙星浓度范围0～40μg/ml下均能够生长,但NaCl、沙拉沙星对其生长具有较大的抑制作用。     

植物提取物微生物限量标准及检测技术进展

植物提取物的微生物检测是保证植物产品安全性的重要手段, 制定严格的植物提取物质量控制标准体系, 特别是功能性食品、食品添加剂和植物源日用化学品等产品中微生物的检测和控制, 对产品的质量及安全保证具有重要作用, 是影响植物提取业实现全面发展的关键问题。本文主要介绍了部分国家植物提取物的微生物限量标准和植物提取物微生物检测的国内外现状与发展趋势, 并就如何建立植物提取物微生物检测行业标准体系提出了若干建议。希望对我国植物提取业实现新时期跨越式发展提供参考。     

蛋白质空间结构属性与全基因组微生物耐热性的关系

 溶剂接触表面积、空腔个数和体积、紧密度、疏水性以及温度因子是影响蛋白质耐热的主要三维结构参数.挑选NCBI COG数据库中具有全基因组的单细胞微生物,选择其中三维结构已知的蛋白质作为研究对象,分析这些因素对细菌类和古细菌类微生物耐热性的影响.结果表明：（1）古细菌类蛋白质的空腔个数和体积与耐热性无关,极性面积和表面残基个数随耐热性增加而降低;（2）超高温细菌类蛋白质的分子量比较小,空腔个数和体积都小于常温蛋白质,而且空腔个数对稳定性的贡献大于空腔体积;（3）无论是古细菌还是细菌类蛋白质,疏水性和紧密度都不随耐热性变化,但暴露残基个数越多,蛋白质的耐热性越差;（4）两类蛋白质侧链的温度因子都高于主链,这与侧链的运动性（柔性）一般比主链高的实验结果一致;另外,超高温细菌类蛋白质的温度因子明显高于常温蛋白质.