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91.
92.
The competitive superiority of invasive plants plays a key role in the process of plant invasions, enabling invasive plants to overcome the resistance of local plant communities. Fast aboveground growth and high densities lead to the competitive superiority of invasive species in the competition for light. However, little is understood of the role belowground root competition may play in invasion. We conducted an experiment to test the effect of root growth on the performance of an invasive shrub Cassia alata, a naturalized, non-invasive shrub Corchorus capsularis, and a native shrub Desmodium reticulatum. We compared seedling growth of the three species and their competitive ability in situ. The roots of the C. alata seedlings grew much faster than those of C. capsularis and D. reticulatum during the entire growth period although C. alata had shorter shoots than D. reticulatum. Furthermore, C. alata showed an apparent competition advantage compared to the other two species as evidenced by less biomass reduction in intraspecific competition and higher competitive effects in interspecific competition. Our study reveals that fast seedling root growth may be important in explaining the competitive advantages of invasive plants. Future studies should pay more attention to the belowground traits of invasive plants, the trade-off between shoot and root growth, and the role of root competition in affecting the population dynamics of invasive plants and the structures of invaded communities. 相似文献
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95.
L. W. Zhu P. Zhao X. A. Cai X. P. Zeng G. Y. Ni J. Y. Zhang L. L. Zou T. T. Mei M. H. Yu 《Trees - Structure and Function》2012,26(2):535-542
Stem CO2 efflux (E
s) has been estimated from a temperature-related equation, but sap flux often affects measurements of E
s, which leads to misunderstanding real stem respiration. In order to observe the relationship between E
s and stem temperature and to analyze the effect of sap velocity on E
s, stem temperature, E
s and sap flux were measured from a subtropical Schima superba plantation in South China on three trees for consecutive 3 days in July and October 2009. Stem temperature, E
s and sap velocity were significantly higher in July than in October. Stem temperature could explain 17–41 and 54–75% variations
of E
s in July and October, respectively. A negative relationship between E
s and stem temperature was found during 1800–2300 hours in July. The daytime E
s was 9.2, 4.3 and 2.4% higher than the predicted for three trees in July, and this occurred only on Tree 1 in October. Sap
velocity was positively correlated with E
s for three trees in July, and the increase of E
s with the increase of sap velocity was only observed on Tree 1 in October. These results demonstrated that the occurrence
of sap flux could account for the increase of daytime E
s, and the effect of sap velocity on E
s varied with the seasons from the S. superba stem. 相似文献
96.
Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen 总被引:1,自引:0,他引:1
The aim of the present study was to determine the influence of chicken semen cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activities. Pooled semen from 10 Black Minorca roosters was used in the study. Semen samples were subjected to cryopreservation using the “pellet” method and dimethylacetamide (DMA) as a cryoprotectant. In the fresh and the frozen-thawed semen sperm membrane integrity (SYBR-14/propidium iodide (PI)), acrosomal damage (PNA-Alexa Fluor®488) and mitochondrial activity (Rhodamine 123) were assessed using flow cytometry. Malondialdehyde (MDA) concentration, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in sperm cells and seminal plasma by spectrophotometry. All sperm characteristics evaluated using flow cytometry were affected by cryopreservation. After freezing-thawing, there was significant (P < 0.01) reduction in sperm membrane integrity, sperm acrosome integrity and mitochondrial activity. Following cryopreservation, MDA concentration significantly increased in chicken seminal plasma and spermatozoa (P < 0.01, P < 0.05). The CAT activity in seminal plasma significantly decreased (P < 0.05), while intracellular activity of this enzyme did not significantly change in frozen-thawed semen. In seminal plasma of frozen-thawed semen the significant increase (P < 0.01) in GPx activity was detected. Whereas GPx activity in spermatozoa remained statistically unchanged after thawing. The SOD activity significantly increased (P < 0.01) in cryopreserved seminal plasma with simultaneous decrease (P < 0.01) of its activity in cells. In conclusion, this is probably the first report describing the level of antioxidant enzymes in frozen-thawed avian semen. The present study showed that the activity of CAT, GPx and SOD in chicken semen was affected by cryopreservation, what increased the intensity of lipid peroxidation (LPO). Catalase appeared to play an important role in the sperm antioxidant defense strategy at cryopreservation since, opposite to SOD and GPx, its content was clearly reduced by the cryopreservation process. Change in the antioxidant defense status of the chicken spermatozoa and surrounding seminal plasma might affect the semen quality and sperm fertilizing ability. 相似文献
97.
The intracellular ice formation (IIF) behavior of Haliotis diversicolor (small abalone) eggs is investigated in this study, in relation to controlling the cooling rate and the concentration of dimethyl sulfoxide (DMSO). The IIF phenomena are monitored under a self-developed thermoelectric cooling (TEC) cryomicroscope system which can achieve accurate temperature control without the use of liquid nitrogen. The accuracy of the isothermal and ramp control is within ±0.5 °C. The IIF results indicate that the IIF of small abalone eggs is well suppressed at cooling rates of 1.5, 3, 7 and 12 °C/min with 2.0, 2.5, 3.0 and 4.0 M DMSO in sea water. As 2.0 M DMSO in sea water is the minimum concentration that has sufficient IIF suppression, it is selected as the suspension solution for the cryopreservation of small abalone eggs in order to consider the solution’s toxicity effect. Moreover, IIF characteristics of the cumulative probability of IIF temperature distribution are shown to be well fitted by the Weibull probabilistic distribution. According to our IIF results and the Weibull distribution parameters, we conclude that cooling at 1.5 °C/min from 20 to −50 °C with 2.0 M DMSO in sea water is more feasible than other combinations of cooling rates and DMSO concentrations in our experiments. Applying this protocol and observing the subsequent osmotic activity, 48.8% of small abalone eggs are osmotically active after thawing. In addition, the higher the cooling rate, the less chance of osmotically active eggs. A separate fertility test experiment, with a cryopreservation protocol of 1.5 °C/min cooling rate and 2.0 M DMSO in sea water, achieves a hatching rate of 23.7%. This study is the first to characterize the IIF behavior of small abalone eggs in regard to the cooling rate and the DMSO concentration. The Weibull probabilistic model fitting in this study is an approach that can be applied by other researchers for effective cryopreservation variability estimation and analysis. 相似文献
98.
Zhigang Chen Xin He Wenjie Xia Qi Huang Zhigang Zhang Jun Ye Chao Ni Pin Wu Dang Wu Jinghong Xu Fuming Qiu Jian Huang 《PloS one》2013,8(12)
Background
The prognostic value of HIFs in colorectal cancer was evaluated in a large number of studies, but the conclusions were inconclusive. Meanwhile, clinicopathologic differences of HIF-1α and HIF-2α were rarely compared in recent studies.Methodology
Identical search strategies were used to search relevant literatures in the PubMed and Web of Science databases. The prognostic significances and clinicopathological differences of HIFs in CRC were analyzed.Principal Findings
A total of 23studies comprising 2984 CRC patients met the inclusion criteria. The results indicated that overexpressed HIFs were significantly associated with increase of mortality risk, including overall survival (OS) (HR 2.06 95%CI 1.55–2.74) and disease free survival (HR 2.84, 95%CI 1.87–4.31). Subgroup analysis revealed that both overexpressed HIF-1α and HIF-2α had correlations with worse prognosis. The pooled HRs were 2.01 (95% CI: 1.55–2.6) and 2.07(95% CI: 1.01–4.26). Further subgroup analysis on HIF-1α was performed by study location, number of patients, quality score and cut-off value. The results showed that HIF-1α overexpression was significantly associated with poor OS, particularly in Asian countries (HR 2.3, 95% CI: 1.74–3.01), while not in European or other countries. In addition, overexpression of HIF-1α was closely related with these clinicopathological features, including Dukes'' stages (OR 0.39, 95% CI: 0.17–0.89), UICC stages (OR 0.42 95% CI: 0.3–0.59), depth of invasion (OR 0.71, 95% CI: 0.51–0.99), lymphnode status (OR 0.49, 95% CI: 0.32–0.73) and metastasis (OR 0.29, 95% CI: 0.11–0.81). While overexpression of HIF-2α was only associated with grade of differentiation (OR 0.48, 95% CI: 0.29–0.81).Conclusions
This study showed that both HIF-1α and HIF-2α overexpression were associated with an unfavorable prognosis. HIF-1α overexpression seemed to be associated with worse prognosis in Asian countries. Additionally, HIF-1α and HIF-2α indicated distinct clinicopathologic features. 相似文献99.
Ng HoiMan Zhang Teng Wang Guoliang Kan SiMeng Ma Guoyi Li Zhe Chen Chang Wang Dandan Wong MengIn Wong ChioHang Ni Jinliang Zhang Xiaohua Douglas 《中国病毒学》2021,36(5):1144-1153
Virologica Sinica - Influenza is one of the major respiratory diseases in humans. Macau is a tourist city with high density of population and special population mobility. The study on the... 相似文献
100.
To investigate the water-air diffusive greenhouse gases (GHGs) fluxes from the Three Gorges Reservoir (TGR), a field experiment on carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes from water surface was carried out from March 2011 to August 2012 by floating static chamber method. The results showed that CO2 was released to the atmosphere all the time and was less in autumn than in other seasons (P < 0.05). CH4 was also released to the atmosphere throughout the year but more in summer than other three seasons (P < 0.05). N2O flux was higher in autumn than other seasons (P < 0.05), and N2O was absorbed from the atmosphere mainly in summer. Moreover, correlation analysis illustrated that CO2 flux had significantly negative correlation with wind velocity (P < 0.05), whereas positive correlation with pH (P < 0.01) had been found. There was no significant correlation between CH4 (or N2O) flux and the measured environmental variables respectively (P > 0.05). Additionally, the annual fluxes of CO2, CH4 and N2O were 140.45 ± 12.57 mg CO2·m?2 h?1, 1.35 ± 0.14 mg CH4·m?2 h?1 and 34.34 ± 11.64 μg N2O·m?2 h?1, respectively. When compared to other reservoirs worldwide, the CO2 and N2O fluxes from TGR were higher than those from boreal and temperate reservoirs, but much lower than those from tropical reservoirs. CH4 flux was lower than those from boreal, temperate and most tropical reservoirs. In our study, the surface area of the TGR emitted 1.42 × 106 t CO2, 1.19 × 104 t CH4 and 589.93 t N2O in a year. The total GWP was 17.68 t CO2-eq ha?1 yr?1, of which CO2 flux was dominant (74.38%). Therefore, CO2 was the main contributor of GHGs fluxes in our study and thus future researches should focus on how to reduce CO2 fluxes from the surface of the TGR. TGR has a considerable contribution to regional GHG emissions. 相似文献