四川威远恐龙足迹一新属

<正>1985年,自贡恐龙博物馆的朱时达、高人彦、彭光照根据四川威远县荣胜乡沙坝村村民李元吉、李鸣章的报告和地矿部第二地质大队提供的资料,在威远县荣胜乡老鸦坡下侏罗统珍珠冲组下部黄色砂岩上发掘出6个连续恐龙足迹化石,并把这批标本运回自贡恐龙博物馆保存。     

数学分析方法在水稻螟虫测报上的应用研究

本利用福建省将乐县1963-1994年测报积累的观察赍料和气象资料,建立了越冬代二化螟、三化螟蛾高峰逐步回归顶测模型．Fuzzy分析预报模型,四代三化螟发生期发生量璜测模型。     

YphC and YsxC GTPases assist the maturation of the central protuberance,GTPase associated region and functional core of the 50S ribosomal subunit

YphC and YsxC are GTPases in Bacillus subtilis that facilitate the assembly of the 50S ribosomal subunit, however their roles in this process are still uncharacterized. To explore their function, we used strains in which the only copy of the yphC or ysxC genes were under the control of an inducible promoter. Under depletion conditions, they accumulated incomplete ribosomal subunits that we named 45S_YphC and 44.5S_YsxC particles. Quantitative mass spectrometry analysis and the 5–6 Å resolution cryo-EM maps of the 45S_YphC and 44.5S_YsxC particles revealed that the two GTPases participate in the maturation of the central protuberance, GTPase associated region and key RNA helices in the A, P and E functional sites of the 50S subunit. We observed that YphC and YsxC bind specifically to the two immature particles, suggesting that they represent either on-pathway intermediates or that their structure has not significantly diverged from that of the actual substrate. These results describe the nature of these immature particles, a widely used tool to study the assembly process of the ribosome. They also provide the first insights into the function of YphC and YsxC in 50S subunit assembly and are consistent with this process occurring through multiple parallel pathways, as it has been described for the 30S subunit.     

Progesterone and DNA damage encourage uterine cell proliferation and decidualization through up-regulating ribonucleotide reductase 2 expression during early pregnancy in mice

Embryo implantation into the maternal uterus is a crucial step for the successful establishment of mammalian pregnancy. Following the attachment of embryo to the uterine luminal epithelium, uterine stromal cells undergo steroid hormone-dependent decidualization, which is characterized by stromal cell proliferation and differentiation. The mechanisms underlying steroid hormone-induced stromal cell proliferation and differentiation during decidualization are still poorly understood. Ribonucleotide reductase, consisting of two subunits (RRM1 and RRM2), is a rate-limiting enzyme in deoxynucleotide production for DNA synthesis and plays an important role in cell proliferation and tumorgenicity. Based on our microarray analysis, Rrm2 expression was significantly higher at implantation sites compared with interimplantation sites in mouse uterus. However, the expression, regulation, and function of RRM2 in mouse uterus during embryo implantation and decidualization are still unknown. Here we show that although both RRM1 and RRM2 expression are markedly induced in mouse uterine stromal cells undergoing decidualization, only RRM2 is regulated by progesterone, a key regulator of decidualization. Further studies showed that the induction of progesterone on RRM2 expression in stromal cells is mediated by the AKT/c-MYC pathway. RRM2 can also be induced by replication stress and DNA damage during decidualization through the ATR/ATM-CHK1-E2F1 pathway. The weight of implantation sites and deciduoma was effectively reduced by specific inhibitors for RRM2. The expression of decidual/trophoblast prolactin-related protein (Dtprp), a reliable marker for decidualization in mice, was significantly reduced in deciduoma and steroid-induced decidual cells after HU treatment. Therefore, RRM2 may be an important effector of progesterone signaling to induce cell proliferation and decidualization in mouse uterus.     

KIRA指标的拓展及其在中国植被与气候关系研究中的应用

根据Kira以月平均气温5℃为界的热量指数和干湿度指数概念,提出了以月平均气温10℃为界的生物热量指数,包括生物温暖指数(BWI)和生物寒冷指数(BCI),并修正其干湿度指数为生物干湿度指数(BK).利用中国689个标准气象台站的资料,分析我国主要植被类型分布与热量因子和干湿度因子的关系,得出两者之间较好的相关性,生物温暖指数、寒冷指数和干湿度指数的散点图较好地表现了中国各植被类型与气候指标的关系和格局.以10℃为界的生物温暖指数不仅对我国森林植被的地理分布和温度气候带的划分具有较好的指示作用,而且对西南部高山、亚高山地区的植被与气候关系指示性较强;生物寒冷指数则对亚热带和热带的指示性很好,能够较好区分亚热带南部及热带地区;由热量指数和降水量综合得出的生物干湿度指数,对中国西北部干旱、半干旱区以至全国的植被分布与水分、热量因子的关系分析有较好的应用价值.     

一株毛栓菌静置液体发酵产纤维素酶条件研究

考察一株新分离的毛栓菌静置产纤维素酶的条件,通过单因素试验和正交设计试验分别对培养基初始pH、培养基中的碳源、氮源及诱导物进行研究。利用DNS（3,5-二硝基水杨酸）法测定CMC-Na（羧甲基纤维素钠）纤维素酶活。结果表明：该毛栓菌静置发酵产纤维素酶的最适pH为5.0,最适碳源、氮源和诱导物分别是淀粉、酵母粉和干麸皮,且质量浓度分别为淀粉1%、酵母粉0.4%、干麸皮1.5%,在此条件下,进行静置发酵3d,可获得CMC-Na（羧甲基纤维素钠）纤维素酶活力最高为56.62U/mL。此研究开创了纤维素酶生产菌的新发现,与以往所报道的好氧产纤维素酶菌种黑曲霉、木霉等相比,具有发酵时间短、耗能小等优点,具有一定的潜在应用价值。     

磷脂酶A2亚型及其与男性生殖相关性研究

磷脂酶A2(phospholipase A2,PLA2)是参与细胞代谢活动、信号转导等过程的重要酶类之一,广泛分布于哺乳动物机体内。随着对磷脂酶A2研究的深入,目前已有30余种磷脂酶A2亚型被逐一鉴定,磷脂酶A2亚型与男性生殖相关性研究也有了新的发现。该文通过对磷脂酶A2的最新亚型分类及其特征、磷脂酶A2在男性生殖系统中的分布、磷脂酶A2参与精子顶体反应及其信号转导通路以及与男性生殖相关的磷脂酶A2亚型等内容进行分析,研究磷脂酶A2在男性生殖系统中发挥的作用及其机制,为男性不育的诊断和治疗提供理论依据和思路策略。     

转油菜素内酯合成基因DET2烟草对NaCI胁迫的反应

通过农杆菌介导法,将含有油菜素内酯合成基因DET2的植物表达栽体pCAMBIA2301-DET2转入烟草,获得转基因烟草植株.用T1代转基因阳性株进行耐NaCl试验,结果显示,NaCl胁迫下转基因烟草、非转基因对照烟草的出苗率、幼苗鲜重、株高及根长均随NaCl浓度增加而下降.但在相同NaCl浓度下,转基因植株鲜重、株高及根长均明显高于非转基因对照烟草,并且转基因植株的丙二醛( MDA)含量低于非转基因植株,诱导蛋白基因P5CS表达高峰出现时间晚于非转基因植株.说明DET2的表达提高了烟草的耐NaCl能力.     

ICAM在着床期小鼠PBLC及EC/DC中表达的研究(英文)

细胞粘附分子（ＣＡＭ）可介导细胞间及细胞与间质之间的相互作用并传导信息,参与机体胚胎发育、免疫调节、炎症反应、组织修复及肿瘤转移等生理和病理过程。细胞间粘附分子１（ＩＣＡＭ１）是主要的ＣＡＭ分子之一,可表达于活化的细胞,内皮细胞等。人膜是母体与胚胎滋养层直接接触的特殊组织、已发现蜕膜细胞在着床过程中参与了局部免疫耐受的形成,但对着床期ＩＣＡＭ１在蜕膜细胞表达的动态研究鲜见报道。本研究采用免疫荧光、多参数流式细胞术,分别从整体和局部角度、着床过程中外周血淋巴细胞（ＰＢＬＣ）及子宫内膜／蜕膜（ＥＣ／ＤＣ）细胞ＩＣＡＭ１的不同表达特点进行了动态观察和对比性分析。结果发现,ＩＣＡＭ１在着床期ＰＢＬＣ及ＥＣ／ＤＣ中的表达均存在明显的动态变化（Ｔａｂ．１;Ｆｉｇｓ．１＆２）。ＩＣＡＭ１在ＰＢＬＣ中的表达于妊娠第一天（Ｄ１）即开始降低,Ｄ２降至最低;与此不同,ＩＣＡＭ１在ＥＣ／ＤＣ中的表达于Ｄ２开始降低,Ｄ４降至最低,Ｄ５开始恢复,但尚未恢复到对照水平。结果表明,ＩＣＡＭ１在蜕膜局部的表达调节方式不同于外周血;ＩＣＡＭ１表达阳性的外周血,淋巴细胞和蜕膜细胞均代表着活化的功能性细胞,这些细胞表面ＩＣＡＭ