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91.
The nasomaxillary epithelial inlay skin graft described by H. D. Gillies in 1923 is one of the historical procedures of the twentieth century. Nowadays, this method has been thoroughly abandoned, and no long-term follow-up has been reported. In the Tokyo Metropolitan Police Hospital, between 1962 and 1983, 51 patients with saddle nose were treated using this procedure and a specially devised outer prosthesis. A follow-up study of these cases was attempted and that of nine cases was carried out. Six of nine patients have been using the outer prosthesis without any serious complications and are satisfied to varying extent. Three patients underwent further surgical treatment using autografts because of serious complications, which included full-thickness skin necrosis, refractory ulcer of the dorsum of nose, and anxiety about the unphysiological communication between the nasal and oral cavities. 相似文献
92.
Rapid increase of vacuolar volume in response to salt stress 总被引:15,自引:0,他引:15
Mimura T Kura-Hotta M Tsujimura T Ohnishi M Miura M Okazaki Y Mimura M Maeshima M Washitani-Nemoto S 《Planta》2003,216(3):397-402
Suspension-cultured cells of mangrove [Bruguiera sexangula (Lour.) Poir.] showed a rapid increase in vacuolar volume under salt stress, although there was no change in the cell volume. The rapid increase in the vacuolar volume was an active process, which followed the activation of the tonoplast H(+)-ATPase and the vacuolar acid phosphatase. The same phenomenon was observed in barley (Hordeum vulgare L. cv. Doriru) root meristematic cells under salt stress but not in pea ( Pisum sativum L.). Increases in vacuolar volume could potentially protect the cytoplasm by decreasing the cytoplasmic volume during the initial phases of salt stress. 相似文献
93.
Autoantibodies against cardiac troponin I are responsible for dilated cardiomyopathy in PD-1-deficient mice 总被引:19,自引:0,他引:19
Okazaki T Tanaka Y Nishio R Mitsuiye T Mizoguchi A Wang J Ishida M Hiai H Matsumori A Minato N Honjo T 《Nature medicine》2003,9(12):1477-1483
We recently reported that mice deficient in the programmed cell death-1 (PD-1) immunoinhibitory coreceptor develop autoimmune dilated cardiomyopathy (DCM), with production of high-titer autoantibodies against a heart-specific, 30-kDa protein. In this study, we purified the 30-kDa protein from heart extract and identified it as cardiac troponin I (cTnI), encoded by a gene in which mutations can cause familial hypertrophic cardiomyopathy (HCM). Administration of monoclonal antibodies to cTnI induced dilatation and dysfunction of hearts in wild-type mice. Monoclonal antibodies to cTnI stained the surface of cardiomyocytes and augmented the voltage-dependent L-type Ca2+ current of normal cardiomyocytes. These findings suggest that antibodies to cTnI induce heart dysfunction and dilatation by chronic stimulation of Ca2+ influx in cardiomyocytes. 相似文献
94.
Identification of liver X receptor-retinoid X receptor as an activator of the sterol regulatory element-binding protein 1c gene promoter 总被引:18,自引:0,他引:18 下载免费PDF全文
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The peptide synthesis from N-acetyl-L-phenylalanine ethyl ester with alaninamide catalyzed by a surfactant-protease complex has been performed in anhydrous hydrophilic organic solvents. Proteases derived from various sources were converted to surfactant-coated complexes with a nonionic surfactant. The surfactant-subtilisin Carlsberg (STC) complex had a higher enzymatic activity than the other protease complexes and the initial reaction rate in tert-amyl alcohol was 26-fold that of STC lyophilized from an optimum aqueous buffer solution. Native STC hardly catalyzed the same reaction. The addition of water to the reaction medium activated the lyophilized STC, however, the reaction rate was much lower than that of the STC complex, and a hydrolysis reaction preferentially proceeded. The STC complex exhibited a high catalytic activity in hydrophilic organic solvents (e.g. tertiary alcohol). The addition of dimethylformamide as a cosolvent improved the solubility of amino acid amides and further activated the STC complex due to the water mimicking effect. When hydrophilic amino acid amides were employed as an acyl acceptor, the peptide formation proceeded efficiently compared to that using hydrophobic substrates. The surfactant-STC complex is a powerful biocatalyst for peptide synthesis because the STC complexes display a high catalytic activity in anhydrous hydrophilic organic solvents and did not require the excess amount of water. Thus the side (hydrolysis) reaction is effectively suppressed and the yield in the dipeptide formation is considerably high. 相似文献
100.
Masayuki Mori Shingo Akiyoshi Yosuke Mizuno Hisato Okuizumi Yasushi Okazaki Yoshihide Hayashizaki Masahiko Nishimura 《Mammalian genome》1998,9(9):695-709
We have applied the restriction landmark genomic scanning (RLGS) method to the SMXA recombinant inbred (RI) mouse strain
set to reveal its detailed genetic profile. A total of 663 polymorphic RLGS spot loci were identified, 576 of which were assigned
to chromosomes. Strain distribution patterns (SDPs) at 55 microsatellite marker loci were also obtained. As a result, the
total number of loci with distinct SDPs on chromosomes increased to 400. These loci were dispersed on all chromosomes, except
for the Chromosome (Chr) Y, and effectively covered the genome with an average spacing of 4 cM. The SMXA RI strain set, hereby,
would be of value for genetic study.
Received: 20 February 1998 / Accepted: 19 May 1998 相似文献