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156.
Navalkar, R. G. (University of Wisconsin, Madison), E. Wiegeshaus, E. Kondo, H. K. Kim, and D. W. Smith. Mycoside G, a specific glycolipid in Mycobacterium marinum (Balnei). J. Bacteriol. 90:262-265. 1965.-A new specific glycolipid in extracts prepared from strains designated Mycobacterium marinum and M. balnei has been demonstrated by use of the techniques of column chromatography and infrared spectroscopy. Since there is now agreement among many workers that M. marinum and M. balnei are identical, the demonstration of the same specific glycolipid in both species is not surprising. This substance, which we have designated mycoside G, is chemically similar to mycosides A and B, and apparently differs only in the sugar moiety. In addition, the lipids extracted from these cultures contain phthiocerol dimycocerosate, a wax component found also in M. tuberculosis and M. bovis. 相似文献
157.
Hydrophilic solute transport across rat alveolar epithelium 总被引:1,自引:0,他引:1
Berg M. M.; Kim K. J.; Lubman R. L.; Crandall E. D. 《Journal of applied physiology》1989,66(5):2320-2327
Diffusional fluxes of a series of hydrophilic nonelectrolytes (molecular radii ranging from 0.15 to 0.57 nm) were measured across the alveolocapillary barrier in the isolated perfused fluid-filled rat lung. Radiolabeled solutes were lavaged into the distal air spaces of isolated Ringer-perfused lungs, and apparent permeability-surface area products were calculated from the rates of isotope appearance in the recirculating perfusate. These data were used to estimate theoretical equivalent pore radii in the alveolar epithelium, with the assumption of diffusive flow through water-filled cylindrical pores. The alveolar epithelium is best characterized by two pore populations, with small pores (radius 0.5 nm) occupying 98.7% of total pore area and larger pores (radius 3.4 nm) occupying 1.3% of total pore area. Net water flow out of the alveolar space was measured by including an impermeant solute (dextran) in the lavage fluid and measuring its concentration in the alveolar space as a function of time. Under control conditions, net water flow averaged 167 nl/s. When 24 microM terbutaline was added to the perfusate, net water flow increased significantly to 350 nl/s (P less than 0.001). Terbutaline had no effect on the fluxes of either glycerol (which traverses the small pore pathway) or sucrose (which traverses the large pore pathway). These findings indicate that the intact mammalian alveolar epithelium is complex and highly resistant to the flow of solutes and water. 相似文献
158.
Hak Ryul Kim Sook J. Seo Richard T. Mayer 《Archives of insect biochemistry and physiology》1989,10(3):215-228
Two kinds of storage proteins (SP-1, SP-2) were confirmed in hemolymph and fat body of Pieris rapae during metamorphosis. Both proteins were present in high concentrations in the hemolymph during the last larval instar. Hemolymph concentrations of SP-1 and SP-2 dropped after pupation as the proteins were being deposited in fat bodies. SP-2 is present in a larger amount than SP-1. Detailed studies on storage proteins determined their properties, mode of synthesis, and accumulation in the fat body. SP-1 has a molecular weight of 500,000 and consists of one type of subunit (Mr 77,000), while SP-2 has a molecular weight of 460,000 and is composed of two types of subunits (Mr 80,000 and 69,000). The pl values of SP-1 and SP-2 were determined to be 6.97 and 7.06, respectively. Fat body cells from 1-day-old fifth instar larvae synthesized storage proteins in large amounts, whereas those from late prepupae exhibited high protein sequestration. Proteins taken up in fat body accumulated in dense granules during the pupal stage but sharply decreased at the adult stage. Morphological changes in the fat body tissues were observed during the larval-pupal transformation; the nuclei of fat body cells became irregularly shaped, and the boundaries between cells seemed to be obscure. Synthesis, storage, or degradation of storage proteins in fat body during development is closely associated with morphological changes in the tissues. 相似文献
159.
The inhibiting effect of 14 typical creosote compounds on the aerobic degradation of toluene was studied in batch experiments. Four NSO-compounds (pyrrole, 1-methylpyrrole, thiophene, and benzofuran) strongly inhibited the degradation of toluene. When the NSO-compounds were present together with toluene, little or no degradation of toluene was observed during 16 days of incubation, compared with a total removal of toluene within 4 days when the four compounds were absent. Indole (an N-compound) and three phenolic compounds (phenol, o-cresol, and 2,4-dimethylphenol) also inhibited the degradation of toluene, though the effect was much weaker that of the four NSO-compounds. O-xylene, p-xylene, naphthalene and 1-methylnaphthalene seemed to stimulate the degradation even though the influence was very weak. No effects of benzothiophene (an S-compound) and quinoline (an N-compound) were observed. Benzofuran (an O-compound) was identified as the compound that most inhibited the degradation of toluene. An effect could be detected even at low concentrations (40 g/l).Abbreviations bf
benzofuran
- bt
benzothiophene
- dmp
2,4-dimethylphenol
- GC
gas chromatograph
- ind
indole
- mnap
1-methylnaphthalene
- MAH
monoaromatic hydrocarbons
- mpyr
1-methylpyrrole
- nap
naphthalene
-
o-cre
o-cresol
-
o-xyl
o-xylene
- phe
phenol
- pyr
pyrrole
-
p-xyl
p-xylene
- tol
toluene
- thi
thiophene
- qui
quinoline 相似文献
160.
Transforming growth factor beta 1 increases IgA isotype switching at the clonal level 总被引:2,自引:0,他引:2
Transforming growth factor beta 1 (TGF beta 1) has important effects on expression of the IgA isotype. TGF beta 1 alone, or in combination with IL-5 or IL-2 increases IgA secretion by populations of LPS-activated surface IgA negative (sIgA-) spleen B cells, while concurrently decreasing IgM and IgG secretion. The present study demonstrates the activity of TGF beta 1 as an IgA isotype switch factor at the clonal level. Stimulation of LPS-activated sIgA- spleen B cell populations with TGF beta 1, or a combination of TGF beta 1 and IL-2, resulted in a significant increase in total numbers of IgA secreting cells, and this increase ultimately was paralleled by an increase in total IgA secretion. Using limiting dilution analysis, TGF beta 1 was shown to increase the frequency of IgA secreting B cell clones, by approximately 20-fold. This was not accompanied by increased numbers of IgA secreting cells/clone. In contrast, IL-2 does not have activity as an IgA switch factor, but does increase IgA production by B cells already committed to secrete that isotype. Cell cycle inhibitors such as thymidine and hydroxyurea also selectively increased numbers of IgA secreting cells and total IgA secretion among populations of LPS-activated sIgA- spleen B cells. This suggests the IgA enhancing activity of TGF beta 1 may, in part, be related to its ability to inhibit cell growth. 相似文献