全文获取类型
收费全文 | 279篇 |
免费 | 11篇 |
出版年
2023年 | 1篇 |
2022年 | 7篇 |
2021年 | 17篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 16篇 |
2017年 | 2篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 10篇 |
2013年 | 8篇 |
2012年 | 15篇 |
2011年 | 11篇 |
2010年 | 4篇 |
2009年 | 10篇 |
2008年 | 11篇 |
2007年 | 16篇 |
2006年 | 13篇 |
2005年 | 10篇 |
2004年 | 3篇 |
2003年 | 6篇 |
2001年 | 1篇 |
2000年 | 6篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 10篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1982年 | 1篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1978年 | 7篇 |
1976年 | 1篇 |
1975年 | 4篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1969年 | 1篇 |
排序方式: 共有290条查询结果,搜索用时 15 毫秒
81.
Genetic Diversity and Biological Control Activity of Novel Species of Closely Related Pseudomonads Isolated from Wheat Field Soils in South Australia 总被引:7,自引:1,他引:6
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ian L. Ross Younes Alami Paul R. Harvey Wafa Achouak Maarten H. Ryder 《Applied microbiology》2000,66(4):1609-1616
Rhizobacteria closely related to two recently described species of pseudomonads, Pseudomonas brassicacearum and Pseudomonas thivervalensis, were isolated from two geographically distinct wheat field soils in South Australia. Isolation was undertaken by either selective plating or immunotrapping utilizing a polyclonal antibody raised against P. brassicacearum. A subset of 42 isolates were characterized by amplified 16S ribosomal DNA restriction analysis (ARDRA), BIOLOG analysis, and gas chromatography-fatty acid methyl ester (GC-FAME) analysis and separated into closely related phenetic groups. More than 75% of isolates tested by ARDRA were found to have >95% similarity to either Pseudomonas corrugata or P. brassicacearum-P. thivervalensis type strains, and all isolates had >90% similarity to either type strain. BIOLOG and GC-FAME clustering showed a >70% match to ARDRA profiles. Strains representing different ARDRA groups were tested in two soil types for biological control activity against the soilborne plant pathogen Gaeumannomyces graminis var. tritici, the causative agent of take-all of wheat and barley. Three isolates out of 11 significantly reduced take-all-induced root lesions on wheat plants grown in a red-brown earth soil. Only one strain, K208, was consistent in reducing disease symptoms in both the acidic red-brown earth and a calcareous sandy loam. Results from this study indicate that P. brassicacearum and P. thivervalensis are present in Australian soils and that a level of genetic diversity exists within these two novel species but that this diversity does not appear to be related to geographic distribution. The result of the glasshouse pot trial suggests that some isolates of these species may have potential as biological control agents for plant disease. 相似文献
82.
Seifan Mostafa Sarmah Ajit K. Samani Ali Khajeh Ebrahiminezhad Alireza Ghasemi Younes Berenjian Aydin 《Applied microbiology and biotechnology》2018,102(10):4489-4498
Applied Microbiology and Biotechnology - Concrete is arguably one of the most important and widely used materials in the world, responsible for the majority of the industrial revolution due to its... 相似文献
83.
Erfani Mehran Zamani Mozhdeh Hosseini Seyed Younes Mostafavi-Pour Zohreh Shafiee Sayed Mohammad Saeidnia Mohammadreza Mokarram Pooneh 《Molecular biology reports》2021,48(10):6749-6756
Molecular Biology Reports - Metastasis is a major cause of death in Colorectal cancer (CRC) patients, and the Epithelial–mesenchymal transition (EMT) has been known to be a crucial event in... 相似文献
84.
ATP translocation into mitochondria isolated from halothane-sensitive pig (HP) muscle was dramatically reduced compared with normal pigs (NP). To determine if this was due to a decreased amount of ATP translocase in the mitochondrial membranes, or a structural modification of this protein, an electrophoretic study was undertaken. Total proteins and purified translocase preparations from (NP) and (HP) mitochondria were analyzed by SDS gel electrophoresis. In the two types of mitochondria no significant differences were observed either in the amount of ATP translocase or in the molecular weight. Also, neither nonequilibrium pH gradient gel electrophoresis nor the analysis of peptides produced by limited proteolysis revealed any structural difference between the two types of protein. On the basis of these results, the depressed translocase activity observed in (HP) mitochondria cannot be explained by a reduced amount of the nucleotide translocase, nor a structural alteration of this protein. Possible inhibition of (HP) translocase activity by Ca2+ accumulation or by other mechanisms is discussed. 相似文献
85.
86.
Rim Rzem Younes Achouri Etienne Marbaix Olivier Schakman Elsa Wiame Sandrine Marie Philippe Gailly Marie-Fran?oise Vincent Maria Veiga-da-Cunha Emile Van Schaftingen 《PloS one》2015,10(3)
The purpose of the present work was to progress in our understanding of the pathophysiology of L-2-hydroxyglutaric aciduria, due to a defect in L-2-hydroxyglutarate dehydrogenase, by creating and studying a mouse model of this disease. L-2-hydroxyglutarate dehydrogenase-deficient mice (l2hgdh
-/-) accumulated L-2-hydroxyglutarate in tissues, most particularly in brain and testis, where the concentration reached ≈ 3.5 μmol/g. Male mice showed a 30% higher excretion of L-2-hydroxyglutarate compared to female mice, supporting that this dicarboxylic acid is partially made in males by lactate dehydrogenase C, a poorly specific form of this enzyme exclusively expressed in testes. Involvement of mitochondrial malate dehydrogenase in the formation of L-2-hydroxyglutarate was supported by the commensurate decrease in the formation of this dicarboxylic acid when down-regulating this enzyme in mouse l2hgdh
-/- embryonic fibroblasts. The concentration of lysine and arginine was markedly increased in the brain of l2hgdh
-/- adult mice. Saccharopine was depleted and glutamine was decreased by ≈ 40%. Lysine-α-ketoglutarate reductase, which converts lysine to saccharopine, was inhibited by L-2-hydroxyglutarate with a Ki of ≈ 0.8 mM. As low but significant activities of the bifunctional enzyme lysine-α-ketoglutarate reductase/saccharopine dehydrogenase were found in brain, these findings suggest that the classical lysine degradation pathway also operates in brain and is inhibited by the high concentrations of L-2-hydroxyglutarate found in l2hgdh
-/- mice. Pathological analysis of the brain showed significant spongiosis. The vacuolar lesions mostly affected oligodendrocytes and myelin sheats, as in other dicarboxylic acidurias, suggesting that the pathophysiology of this model of leukodystrophy may involve irreversible pumping of a dicarboxylate in oligodendrocytes. Neurobehavioral testing indicated that the mice mostly suffered from a deficit in learning capacity. In conclusion, the findings support the concept that L-2-hydroxyglutaric aciduria is a disorder of metabolite repair. The accumulation of L-2-hydroxyglutarate exerts toxic effects through various means including enzyme inhibition and glial cell swelling. 相似文献
87.
Abdulaziz A Al-Askar Khalid M Ghoneem Younes M Rashad Waleed M Abdulkhair Elsayed E Hafez Yasser M Shabana Zakaria A Baka 《Microbial biotechnology》2014,7(6):556-569
One hundred samples of tomato seeds were collected in 2011 and 2012 from tomato-cultivated fields in Saudi Arabia and screened for their seed-borne mycoflora. A total of 30 genera and 57 species of fungi were recovered from the collected seed samples using agar plate and deep-freezing blotter methods. The two methods differed as regards the frequency of recovered seed-borne fungi. Seven fungi among those recovered from tomato seeds, which are known as plant pathogens, were tested for their pathogenicity and transmission on tomato seedlings. The recovery rate of these pathogens gradually decreased from root up to the upper stem, and did not reach to the stem apex. The distribution of tomato seed-borne fungi was also investigated throughout Saudi Arabia. In this concern, Al-Madena governorate recorded the highest incidence of fungal flora associated with tomato seeds. The impact of meteorological variables on the distribution of tomato seed-borne mycoflora was explored using the ordination technique (canonical correspondence analysis). Among all climatic factors, relative humidity was the most influential variable in this regard. Our findings may provide a valuable contribution to our understanding of future global disease change and may be used also to predict disease occurrence and fungal transfer to new uninfected areas. 相似文献
88.
Elsayed E. Hafez Gamal M. Abdel-Fattah Safwat A. El-Haddad Younes M. Rashad 《Annals of microbiology》2013,63(3):1195-1203
A time course study was conducted to investigate disease development and molecular defense response in common bean (Phaseolus vulgaris L.) plants colonized by a mixture of five arbuscular mycorrhizal (AM) fungi, namely, Glomus mosseae, G. intraradices, G. clarum, Gigaspora gigantea, and Gigaspora margarita, and post-infected with the soil-borne pathogen Rhizoctonia solani. Results showed that pre-colonization of bean plants by AM fungi significantly reduced disease severity and disease incidence. DNA fingerprinting using the differential display technique revealed a genetic polymorphism (86.8 %) in bean plants that resulted from the colonization by AM fungi. Two genetic mechanisms were recorded: (1) switching on of new genes and (2) induction of other active genes, including the defense genes chitinase and β-1,3-glucanase, to a highly expressed state. 相似文献
89.
Mousavi Pegah Morowvat Mohammad Hossein Mostafavi-Pour Zohreh Aram Farzaneh Malekzadeh Kianoosh Nezafat Navid Ghasemi Younes 《International journal of peptide research and therapeutics》2021,27(1):209-218
International Journal of Peptide Research and Therapeutics - Recombinant reteplase is the truncated form of tissue plasminogen activator. Signal peptides play a pivotal role in the secretion of... 相似文献
90.
Roghayeh Sheervalilou Milad Shirvaliloo Nahid Dadashzadeh Sakine Shirvalilou Omolbanin Shahraki Younes Pilehvar-Soltanahmadi Habib Ghaznavi Samideh Khoei Ziba Nazarlou 《Journal of cellular physiology》2020,235(12):8873-8924
Months after the outbreak of a new flu-like disease in China, the entire world is now in a state of caution. The subsequent less-anticipated propagation of the novel coronavirus disease, formally known as COVID-19, not only made it to headlines by an overwhelmingly high transmission rate and fatality reports, but also raised an alarm for the medical community all around the globe. Since the causative agent, SARS-CoV-2, is a recently discovered species, there is no specific medicine for downright treatment of the infection. This has led to an unprecedented societal fear of the newly born disease, adding a psychological aspect to the physical manifestation of the virus. Herein, the COVID-19 structure, epidemiology, pathogenesis, etiology, diagnosis, and therapy have been reviewed. 相似文献