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61.
BJÖRN SÖDER BÄCK 《Freshwater Biology》1995,33(2):291-304
1. The introduced North American crayfish Pacifastacits leniusculus is currently replacing the native crayfish Astacus astacus in a Swedish lake. Using field data from co-occurring populations on life-history traits and population size structure of the two species, this study evaluated possible mechanisms behind the replacement. 2. Pacifastacus showed a higher capacity for population increase; the species had a higher individual growth rate, reached sexual maturity at a smaller size and lower age, and had a higher per capita egg production than Astacus. 3. A higher frequency of non-lethal injuries in Astacus suggested the predominance of Pacifastacus in interference interactions. These results support a competitive exclusion hypothesis. 4. A dramatic change in relative abundance of the two species in recent years was the result of an almost complete cessation in recruitment of young-of-the-year (YOY) in Astacus. Poor recruitment in Astacus was probably caused by the combined effects of interspecific competition and predation, resulting in increased mortality among YOY Astacus, and by reproductive interference, suppressing the less common species. 5. The results support the hypothesis that the observed replacement of Astacus by Pacifastacus is governed by a combination of several interacting mechanisms, of which 相似文献
62.
1. The potency of agonists and competitive antagonists on the two expressed forms of the nicotinic acetylcholine receptor (adult or junctional subtype, -AChR; fetal or extrajunctional subtype, -AChR) have not previously been compared systematically in homogeneous receptor preparations.2. Each subtype of the receptor was expressed separately in Xenopus oocytes by cytoplasmic injection of combinations of RNA transcribed in vitro. The presence of each type of receptor was confirmed by single-channel recordings. Expressing oocytes were assayed using discontinuous, single-electrode voltage clamp by measuring peak currents in response to test compounds.3. The extrajunctional subtype was more potently activated by the nicotinic agonist dimethylphenyl piperazinium iodide (DMPP) than was the junctional form. There was no statistically significant difference in potency between the two subtypes for other nicotinic agonists (nicotine, cytisine and succinylcholine). The rank order of potency for -AChR was succinylcholine>cytisine>DMPP>nicotine, and that for -AChR was DMPP>cytisine>succinylcholine>nicotine.4. Two agonists (cytisine and succinylcholine) displayed six- to eight-fold greater intrinsic activity in activating -AChR over -AChR. There was no difference between the two forms of receptor in efficacy for nicotine.5. The extrajunctional form was much more potently inhibited by the steroidal competitive antagonist pancuronium than was the junctional receptor. However, there was no significant difference in potency of inhibition by the curariform drug atracurium.6. Contrary to previous reports, there is no consistent relation between the effect of agonists and antagonists and the subtype of receptor. These data suggest that the resistance or sensitivity to these agents seen in various clinical settings are related to other cellular factors. 相似文献
63.
Glycerol 3-phosphate acylation in microsomes of type II cells isolated from adult rat lung 总被引:2,自引:0,他引:2
J J Batenburg J N den Breejen R W Yost H P Haagsman L M van Golde 《Biochimica et biophysica acta》1986,878(3):301-309
Glycerol 3-phosphate acylation was studied in type II cells isolated from adult rat lung. The process was found to be largely microsomal. In the microsomes phosphatidic acid is the main product of glycerol 3-phosphate acylation. Glycerol-3-phosphate acyltransferase is rate limiting in the phosphatidic acid formation by the microsomes. Type II cell microsomes incorporate palmitoyl and oleoyl residues into phosphatidic acid at an equal rate if palmitoyl-CoA and oleoyl-CoA are added separately. However, if palmitoyl-CoA and oleoyl-CoA are added as an equimolar mixture the unsaturated fatty acyl moiety is incorporated much faster. Under the latter conditions monoenoic species constitute the most abundant products of glycerol 3-phosphate acylation. The microsomes incorporate both palmitoyl and oleoyl residues readily into both the 1- and 2-position of phosphatidic acid, even when palmitoyl-CoA and oleoyl-CoA are added together. Assuming that both phosphatidic acid phosphatase and cholinephosphotransferase do not discriminate against substrates with an unsaturated acyl moiety at the 1-position and a saturated acyl moiety at the 2-position, the last two observations indicate that a considerable percentage of phosphatidylcholine molecules synthesized de novo may have a saturated fatty acid at the 2-position and an unsaturated fatty acid at the 1-position, and that remodeling at the 1-position may be important for the formation of surfactant dipalmitoylphosphatidylcholine. They also indicate that type II cell microsomes are capable of synthesizing the dipalmitoyl species of phosphatidic acid. However, since there is a preference for the acylation of glycerol 3-phosphate with unsaturated fatty acyl residues, the percentage of dipalmitoyl species in the synthesized phosphatidic acid, and thereby the percentage of dipalmitoyl species in the phosphatidylcholine synthesized de novo, will probably depend on the relative availability of the various acyl-CoA species. 相似文献
64.
IN most work on the problems of releasing radiostrontium which has been incorporated into the vertebrate skeleton the effects of various parenterally administered chemical agents on the excretion rate of radiostrontium have been studied. Someriecrease in skeletal retention was obtained when inactive salts of strontium1–4 or zirconium5 were given almost simultaneously with radiostrontium; so far all other efforts have been practically useless. 相似文献
65.
Plant-soil interactions associated with acid,weathered soils 总被引:1,自引:0,他引:1
Plant-soil interactions in weathered soils are so complex that unqualified statements about a suitable pH for plants are risky.
Conventional experimental designs and statistical methods may not be appropriate for investigating such complexities. Lime
experiments using continuous function designs and observation of plant response to indigenous variability in soil pH permit
detailed observations of plant-soil interactions that are frequently not detected. A graphical boundary-line approach to interpreting
data can make good sense out of apparent confusion.
Increasing the pH of variable-charge soils by adding lime or by indigenous means increased CEC and retarded cation leaching,
but Ca solubility changed very little over the range pH 5 to 6. N fixation and yield was closely related to soil pH, soil
Mn and Mn uptake by soybean. This result was clearly demonstrated regardless of numerous other limiting factors. Plant yield
response curves resolved into distinct segments that corresponded with associated soil properties. Excess Al compounded by
Ca deficiency is suspect in the pH range <5. Excess Mn, and Ca deficiency probably limited yields in the pH range 5.0 to 5.7.
Yields were stable, and Ca and P were constant in the pH interval 5.7 to 6.0. Yields abruptly increased in the pH interval
6.0 to 6.3. This was associated with elevated Ca concentrations in soil solutions. 相似文献
66.
Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae. 总被引:17,自引:3,他引:14
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In the yeast Saccharomyces cerevisiae, the splicing of mRNA precursors is disrupted by a severe heat shock. Mild heat treatments prior to severe heat shock protect splicing from disruption, as was previously reported for Drosophila melanogaster. In contrast to D. melanogaster, protein synthesis during the pretreatment is not required to protect splicing in yeast cells. However, protein synthesis is required for the rapid recovery of splicing once it has been disrupted by a sudden severe heat shock. Mutations in two classes of yeast hsp genes affect the pattern of RNA splicing during the heat shock response. First, certain hsp70 mutants, which overproduce other heat shock proteins at normal temperatures, show constitutive protection of splicing at high temperatures and do not require pretreatment. Second, in hsp104 mutants, the recovery of RNA splicing after a severe heat shock is delayed compared with wild-type cells. These results indicate a greater degree of specialization in the protective functions of hsps than has previously been suspected. Some of the proteins (e.g., members of the hsp70 and hsp82 gene families) help to maintain normal cellular processes at higher temperatures. The particular function of hsp104, at least in splicing, is to facilitate recovery of the process once it has been disrupted. 相似文献
67.
Amino acid-specific ADP-ribosylation. Evidence for two distinct NAD:arginine ADP-ribosyltransferases in turkey erythrocytes 总被引:3,自引:0,他引:3
An NAD:arginine mono-ADP-ribosyltransferase has been purified 270,000-fold from turkey erythrocytes through a five-step chromatographic procedure to apparent electrophoretic homogeneity. Molecular weight determinations of the enzyme by sodium dodecyl sulfate-gel electrophoresis, Ultrogel AcA 54, and TSK 3000 SW gel filtration were consistent with Mr = 32,000. The purified enzyme utilized arginine, other low molecular weight guanidino compounds, and various proteins as ADP-ribose acceptors. The Km values for NAD and arginine methyl ester were 36 and 3,000 microM, respectively. Unlike another transferase purified from turkey erythrocytes (Moss, J., and Stanley, S.J. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 4809-4812), this enzyme was not activated by chaotropic salts or micromolar concentrations of histone. Thus, two distinct soluble ADP-ribosyltransferases may be present in turkey erythrocytes. 相似文献
68.
Guobin He Debanjan Dhar Hayato Nakagawa Joan Font-Burgada Hisanobu Ogata Yuhong Jiang Shabnam Shalapour Ekihiro Seki Shawn E. Yost Kristen Jepsen Kelly A. Frazer Olivier Harismendy Maria Hatziapostolou Dimitrios Iliopoulos Atsushi Suetsugu Robert M. Hoffman Ryosuke Tateishi Kazuhiko Koike Michael Karin 《Cell》2013
69.
ROLF G. BEUTEL FRANK FRIEDRICH ULRIKE ASPÖCK 《Zoological Journal of the Linnean Society》2010,158(3):533-562
External and internal head structures of larvae of Nevrorthidae were described in detail. The results were compared to conditions found in other representatives of Neuroptera and the other two neuropterid orders. The cladistic analysis supported the monophyly of Neuroptera, Neuroptera exclusive of Nevrorthidae, Hemerobiiformia, and Myrmeleontiformia. Neuroptera exclusive of Nevrorthidae are supported by the formation of an undivided postmentum and the presence of cryptonephric Malpighian tubules. The highly specialized articulation of the neck (Rollengelenk) and the absence of a salivary duct are autapomorphies of Nevrorthidae. Ithonidae and Polystoechotidae form a clade and are the sister group of the remaining Hemerobiiformia, which are characterized by the complete lack of a gula and a terminal filament of the antenna. Within this lineage, a clade comprising Mantispidae, Dilaridae, Berothidae, and Rhachiberothidae is well supported. Larvae of Myrmeleontiformia are characterized by a complex transformation of head structures, with a hypostomal bridge, a small triangular gula, largely reduced maxillary grooves, and anteriorly shifted posterior tentorial grooves. The slender finger‐like mid‐dorsal apodeme is another autapomorphy of the group. Psychopsidae are placed as the sister group of the remaining Myrmeleontiformia, which are characterized by a conspicuous, protruding ocular region (often less distinct or even absent in Nemopteridae). Ascalaphidae are the sister group of Myrmeleontidae. Larvae of both families share the fusion of the tibia and tarsus in the hind leg. The larval characters analysed were not sufficient for full resolution of the myrmeleontiform and hemerobiiform lineages. The position of several families such as Osmylidae, Sisyridae, and Coniopterygidae remains uncertain. The results are in agreement with an aquatic ancestor of Neuroptera and secondarily acquired terrestrial habits within the lineage (Neuroptera exclusive of Nevrorthidae), and another invasion of the aquatic environment by Sisyridae. © 2010 The Linnean Society of London, Zoological Journal of the Linnean Society, 2010, 158 , 533–562. 相似文献
70.