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91.
Bornstein B Gottfried Y Edison N Shekhtman A Lev T Glaser F Larisch S 《Apoptosis : an international journal on programmed cell death》2011,16(9):869-881
ARTS (Sept4_i2), is a pro-apoptotic protein localized at the mitochondria of living cells. In response to apoptotic signals,
ARTS rapidly translocates to the cytosol where it binds and antagonizes XIAP to promote caspase activation. However, the mechanism
of interaction between these two proteins and how it is regulated remained to be explored. In this study, we show that ARTS
and XIAP bind directly to each other, as recombinant ARTS and XIAP proteins co-immunoprecipitate together. We also show that
over expression of ARTS alone is sufficient to induce a strong down-regulation of XIAP protein levels and that this reduction
occurs through the ubiquitin proteasome system (UPS). Using various deletion and mutation constructs of XIAP we show that
ARTS specifically binds to the BIR3 domain in XIAP. Moreover, we found that ARTS binds to different sequences in BIR3 than
other IAP antagonists such as SMAC/Diablo. Computational analysis comparing the location of the putative ARTS interface in
BIR3 with the known interfaces of SMAC/Diablo and caspase 9 support our results indicating that ARTS interacts with residues
in BIR3 that are different from those involved in binding SMAC/Diablo and caspase 9. We therefore suggest that ARTS binds
and antagonizes XIAP in a way which is distinct from other IAP-antagonists to promote apoptosis. 相似文献
92.
Busso D Peleg Y Heidebrecht T Romier C Jacobovitch Y Dantes A Salim L Troesch E Schuetz A Heinemann U Folkers GE Geerlof A Wilmanns M Polewacz A Quedenau C Büssow K Adamson R Blagova E Walton J Cartwright JL Bird LE Owens RJ Berrow NS Wilson KS Sussman JL Perrakis A Celie PH 《Journal of structural biology》2011,175(2):159-170
Escherichia coli (E. coli) remains the most commonly used host for recombinant protein expression. It is well known that a variety of experimental factors influence the protein production level as well as the solubility profile of over-expressed proteins. This becomes increasingly important for optimizing production of protein complexes using co-expression strategies. In this study, we focus on the effect of the choice of the expression vector system: by standardizing experimental factors including bacterial strain, cultivation temperature and growth medium composition, we compare the effectiveness of expression technologies used by the partners of the Structural Proteomics in Europe 2 (SPINE2-complexes) consortium. Four different protein complexes, including three binary and one ternary complex, all known to be produced in the soluble form in E. coli, are used as the benchmark targets. The respective genes were cloned by each partner into their preferred set of vectors. The resulting constructs were then used for comparative co-expression analysis done in parallel and under identical conditions at a single site. Our data show that multiple strategies can be applied for the expression of protein complexes in high yield. While there is no 'silver bullet' approach that was infallible even for this small test set, our observations are useful as a guideline to delineate co-expression strategies for particular protein complexes. 相似文献
93.
Global human footprint on the linkage between biodiversity and ecosystem functioning in reef fishes 总被引:1,自引:0,他引:1
Mora C Aburto-Oropeza O Ayala Bocos A Ayotte PM Banks S Bauman AG Beger M Bessudo S Booth DJ Brokovich E Brooks A Chabanet P Cinner JE Cortés J Cruz-Motta JJ Cupul Magaña A Demartini EE Edgar GJ Feary DA Ferse SC Friedlander AM Gaston KJ Gough C Graham NA Green A Guzman H Hardt M Kulbicki M Letourneur Y López Pérez A Loreau M Loya Y Martinez C Mascareñas-Osorio I Morove T Nadon MO Nakamura Y Paredes G Polunin NV Pratchett MS Reyes Bonilla H Rivera F Sala E Sandin SA Soler G Stuart-Smith R 《PLoS biology》2011,9(4):e1000606
Difficulties in scaling up theoretical and experimental results have raised controversy over the consequences of biodiversity loss for the functioning of natural ecosystems. Using a global survey of reef fish assemblages, we show that in contrast to previous theoretical and experimental studies, ecosystem functioning (as measured by standing biomass) scales in a non-saturating manner with biodiversity (as measured by species and functional richness) in this ecosystem. Our field study also shows a significant and negative interaction between human population density and biodiversity on ecosystem functioning (i.e., for the same human density there were larger reductions in standing biomass at more diverse reefs). Human effects were found to be related to fishing, coastal development, and land use stressors, and currently affect over 75% of the world's coral reefs. Our results indicate that the consequences of biodiversity loss in coral reefs have been considerably underestimated based on existing knowledge and that reef fish assemblages, particularly the most diverse, are greatly vulnerable to the expansion and intensity of anthropogenic stressors in coastal areas. 相似文献
94.
95.
Existing evidence suggests that in social contexts individuals become coupled in their emotions and behaviors. Furthermore, recent biological studies demonstrate that the physiological signals of interacting individuals become coupled as well, exhibiting temporally synchronized response patterns. However, it is yet unknown whether people can shape each other''s responses without the direct, face-to-face interaction. Here we investigated whether the convergence of physiological and emotional states can occur among “merely co-present” individuals, without direct interactional exchanges. To this end, we measured continuous autonomic signals and collected emotional responses of participants who watched emotional movies together, seated side-by-side. We found that the autonomic signals of co-present participants were idiosyncratically synchronized and that the degree of this synchronization was correlated with the convergence of their emotional responses. These findings suggest that moment-to-moment emotional transmissions, resulting in shared emotional experiences, can occur in the absence of direct communication and are mediated by autonomic synchronization. 相似文献
96.
Aya Goldshtein Marine Veits Itzhak Khait Kfir Saban Yuval Sapir Yossi Yovel Lilach Hadany 《Ecology letters》2020,23(9):1423-1425
Ecol. Lett. 22, 2019, 1483 demonstrated, for the first time, a rapid response of a plant to the airborne sounds of pollinators. Pyke et al. argue that this response is unlikely to be adaptive. Here we clarify some misunderstandings, and demonstrate the potential adaptive value using theoretical modelling and field observations. 相似文献
97.
The adhesion molecule CD58 is natively expressed in both a glycosylphosphatidylinositol (GPI)-anchored form and a transmembrane form. We previously demonstrated that the two isoforms of CD58 are differentially distributed in the cell membrane. The GPI-linked form resides in lipid rafts while the transmembrane form resides outside lipid rafts. Following cross-linking a fraction of transmembrane CD58 redistributes to lipid rafts. It has also been demonstrated that ligand binding to CD58 induces biological functions such as cytokine production and immunoglobulin isotype switching, indicating that cell–cell interactions result in CD58-mediated signal transduction. However, the signaling pathways involved in these activation processes are poorly defined. Here we show for the first time that cross-linking of CD58 induces protein tyrosine phosphorylation of BLNK, Syk and PLCγ, and activation of ERK and Akt/PKB. In addition, we studied how these signaling events relate to the distinct membrane localization of the two isoforms of CD58. We demonstrate that cross-linking of CD58 triggers signaling that is predominantly associated with transmembrane CD58 in nonraft microdomains. Moreover, signaling through transmembrane CD58 does not depend on coexpression of the GPI-linked isoform. Thus, despite the residence of its GPI-anchored isoform in lipid rafts and the translocation of a fraction of its transmembrane isoform to lipid rafts, CD58 signaling is triggered by the transmembrane isoform outside lipid rafts. These findings corroborate signaling outside lipid rafts, as opposed to the established notion that rafts function as essential platforms for signaling. 相似文献
98.
Ami Schlesinger Esti Kramarsky-Winter Yossi Loya 《Marine biotechnology (New York, N.Y.)》2009,11(4):441-444
Cnidarian venoms are potentially valuable tools for biomedical research and drug development. They are contained within nematocysts,
the stinging organelles of cnidarians. Several methods exist for the isolation of nematocysts from cnidarian tissues; most
are tedious and target nematocysts from specific tissues. We have discovered that the isolated active nematocyst complement
(cnidome) of several sea anemone (Cnidaria: Anthozoa) species is readily accessible. These nematocysts are isolated, concentrated,
and released to the aqueous environment as a by-product of aeolid nudibranch Spurilla neapolitana cultures. S. neapolitana feed on venomous sea anemones laden with stinging nematocysts. The ingested stinging organelles of several sea anemone species
are effectively excreted in the nudibranch feces. We succeeded in purifying the active organelles and inducing their discharge.
Thus, our current study presents the attractive possibility of using nudibranchs to produce nematocysts for the investigation
of novel marine compounds. 相似文献
99.
Pulse amplitude modulated (PAM) fluorometry has been suggested as a tool for estimating environmental stresses on corals.
However, information regarding natural changes in maximal quantum yields (F
v/F
m) of corals during “normal” (i.e. non-bleaching) years has been limited. In this study, seasonal variations in F
v/F
m for Stylophora pistillata and Favia favus, measured in situ, correlated with seasonal changes in solar irradiance but not in sea temperature. Interactions between
sea temperature and irradiance were further studied by growing these corals and Pocillopora damicornis under controlled conditions. Exposure to high light with normal or high temperatures resulted in lower F
v/F
m values than exposure to low light at both temperatures. Thus, high irradiances may cause decreased F
v/F
m values in corals at least as much as, if not more than, high temperatures. Such seasonal variations should be taken into
account when using PAM fluorometry as a diagnostic tool for predicting coral bleaching. 相似文献
100.
The effects of competition and risk of predation on secondary cavity breeders were examined between the 2008 and 2009 breeding
seasons using an experimental design manipulating two nest entrance sizes (large entrances allowed Barn Owls (Tyto alba) to enter, while the small entrances excluded them). During the 2009 breeding season, the entrance sizes of nest boxes were
exchanged, so that if during one year a nest box in a particular location had a small entrance, the second year it had a large
entrance and vice versa. Barn Owls and Eurasian Kestrels (Falco tinnunculus) occupied 67.3 and 17.3%, respectively, of large entrance nest boxes. Significantly more Jackdaws (Corvus monedula), House Sparrows (Passer domesticus) and Scops Owls (Otus scops) bred in nest boxes with small than with large entrances. After nest box entrance sizes were exchanged, Barn Owls and smaller
species did not breed in the same nest boxes with the new entrance size. Jackdaws probably did not breed in large entrance
nest boxes due both to exploitation competition (Barn Owls and Eurasian Kestrels occupied the majority of large entrance nest
boxes), and may also have avoided empty nest boxes because of the risk of interference competition; whereas smaller species
may have also avoided large entrance nest boxes due to risk of predation. 相似文献