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171.
Forty-four marsupials, 77 rodents and 161 ticks were captured in an Atlantic Forest Reserve in Cotia county, State of S?o Paulo, where human cases of Lyme disease (LD) simile were reported. Twenty-one borrelia-like spirochete isolates were recovered from the mammals' blood and rodent livers or spleens, and triturated ticks inoculated into BSK II medium. Our results suggest that the reservoirs and ticks collected may harbor borrelia-like spirochetes, some of which have an antigenic similarity with the unknown causative agent of LD simile in Brazil, and/or with North American Borrelia burgdorferi s. s. 相似文献
172.
173.
Osamu Yasuhara Akinori Matsuo Jean-Pierre Bellier Yoshinari Aimi 《The journal of histochemistry and cytochemistry》2007,55(3):287-299
Cholinergic innervation of the heart has been analyzed using cholinergic markers including acetylcholinesterase, choline acetyltransferase (ChAT), and vesicular acetylcholine transporter (VAChT). In the present study we demonstrate putative cholinergic nerves in the rat heart using an antibody to ChAT of a peripheral type (pChAT), which is the product of a splice variant of ChAT mRNA and preferentially localized to peripheral cholinergic nerves. Expression of mRNAs for pChAT and the conventional form of ChAT (cChAT) were verified in the rat atrium by RT-PCR. Localization of both protein products in the atrium was confirmed by Western blotting. Virtually all neurons and small intensely fluorescent cells in the intrinsic cardiac ganglia were stained immunohistochemically for pChAT. The density of pChAT-positive fibers was very high in the conducting system, high in both atria, the right atrium in particular, and low in the ventricular walls. pChAT and VAChT immunoreactivities were closely associated in some fibers and fiber bundles in the ventricular walls. These results indicate that intrinsic cardiac neurons homogeneously express both pChAT and cChAT. Furthermore, innervation of the ventricular walls by pChAT- and VAChT-positive fibers provides morphological evidence for a significant role of cholinergic mechanisms in ventricular functions. 相似文献
174.
Daisuke Irikura Chie Monma Yasunori Suzuki Akiko Nakama Akemi Kai Aya Fukui-Miyazaki Yasuhiko Horiguchi Tomoya Yoshinari Yoshiko Sugita-Konishi Yoichi Kamata 《PloS one》2015,10(11)
There is a strain of Clostridium perfringens, W5052, which does not produce a known enterotoxin. We herein report that the strain W5052 expressed a homologue of the iota-like toxin components sa and sb of C. spiroforme, named Clostridium perfringens iota-like enterotoxin, CPILE-a and CPILE-b, respectively, based on the results of a genome sequencing analysis and a systematic protein screening. In the nicotinamide glyco-hydrolase (NADase) assay the hydrolysis activity was dose-dependently increased by the concentration of rCPILE-a, as judged by the mass spectrometry analysis. In addition, the actin monomer of the lysates of Vero and L929 cells were radiolabeled in the presence of [32P]NAD and rCPILE-a. These findings indicated that CPILE-a possesses ADP-ribosylation activity. The culture supernatant of W5052 facilitated the rounding and killing of Vero and L929 cells, but the rCPILE-a or a non-proteolyzed rCPILE-b did not. However, a trypsin-treated rCPILE-b did. Moreover, a mixture of rCPILE-a and the trypsin-treated rCPILE-b enhanced the cell rounding and killing activities, compared with that induced by the trypsin-treated rCPILE-b alone. The injection of the mixture of rCPILE-a and the trypsin-treated rCPILE-b into an ileum loop of rabbits evoked the swelling of the loop and accumulation of the fluid dose-dependently, suggesting that CPILE possesses enterotoxic activity. The evidence presented in this communication will facilitate the epidemiological, etiological, and toxicological studies of C. perfringens food poisoning, and also stimulate studies on the transfer of the toxins’ gene(s) among the Genus Clostridium. 相似文献
175.
Kohchi Y Hattori K Oikawa N Mizuguchi E Isshiki Y Aso K Yoshinari K Shirai H Miwa M Inagaki Y Ura M Ogawa K Okabe H Ishitsuka H Shimma N 《Bioorganic & medicinal chemistry letters》2007,17(8):2241-2245
DNA microarray analysis comparing human tumor tissues with normal tissues including hematopoietic progenitor cells resulted in identification of membrane dipeptidase as a prodrug activation enzyme. Novel prodrugs of 2'-deoxy-2'-methylidenecytidine (DMDC) including compound 23 that are activated by membrane dipeptidase (MDP) preferentially in tumor tissue were designed and synthesized to generate the active drug, DMDC, after hydrolysis of the dipeptide bond followed by spontaneous cyclization of the promoiety. 相似文献
176.
Yoshinari Ando Yoshiko Maida Ayako Morinaga Alexander M Burroughs Ryuichiro Kimura Joe Chiba Harukazu Suzuki Kenkichi Masutomi Yoshihide Hayashizaki 《BMC molecular biology》2011,12(1):6
Background
DICER is an RNase III family endoribonuclease that processes precursor microRNAs (pre-miRNAs) and long double-stranded RNAs, generating microRNA (miRNA) duplexes and short interfering RNA duplexes with 20~23 nucleotides (nts) in length. The typical form of pre-miRNA processed by the Drosha protein is a hairpin RNA with 2-nt 3' overhangs. On the other hand, production of mature miRNA from an endogenous hairpin RNA with 5' overhangs has also been reported, although the mechanism for this process is unknown. 相似文献177.
Shoko Toyama Naoto Tamura Kazuhiko Haruta Takeo Karakida Shigeyuki Mori Tetsuo Watanabe Takao Yamori Yoshinari Takasaki 《Arthritis research & therapy》2010,12(3):R92
Introduction
Targeting joint destruction induced by osteoclasts (OCs) is critical for management of patients with rheumatoid arthritis (RA). Since phosphoinositide 3-kinase (PI3-K) plays a critical role in osteoclastogenesis and bone resorption, we examined the effects of ZSTK474, a novel phosphoinositide 3-kinase (PI3-K)-specific inhibitor, on murine OCs in vitro and in vivo. 相似文献178.
Nobuaki Hori Mutsumi Watanabe Yoshinari Yamazaki Yoichi Mikami 《Bioscience, biotechnology, and biochemistry》2013,77(8):2205-2210
A thermostable purine nucleoside phosphorylase has been purified more than 800-fold from Bacillus stearothermophilus JTS 859. The enzyme had a molecular weight of 68,000 consisting of 2 identical subunits (A/w, 34,000). The isoelectric point of the enzyme was 4.7. The enzyme did not contain cysteine. The optimal pH of the enzyme reaction was from 7.5 to 11.0. The Michaelis constants for inosine, guanosine, 2′-deoxyinosine, and 2′-deoxyguanosine were 0.22, 0.14, 0.20, and 0.10mM, respectively. The optimal temperature of the reaction was 80 C. The half-life of the enzyme was 16 hr in 20mM potassium phosphate and ImM inosine (pH 7.0) at 80°C, and no decrease of the enzyme activity was observed at least for the first 30 hr at 70°C. 相似文献
179.
Tomoya Yoshinari Atsushi Yaguchi Naoko Takahashi-Ando Makoto Kimura Haruo Takahashi Takashi Nakajima Yoshiko Sugita-Konishi Hiromichi Nagasawa Shohei Sakuda 《FEMS microbiology letters》2008,284(2):184-190
The essential oil of German chamomile showed specific inhibition toward aflatoxin G(1) (AFG(1)) production, and (E)- and (Z)-spiroethers were isolated as the active compounds from the oil. The (E)- and (Z)-spiroethers inhibited AFG(1) production of Aspergillus parasiticus with inhibitory concentration 50% (IC(50)) values of 2.8 and 20.8 microM, respectively, without inhibiting fungal growth. Results of an O-methylsterigmatocystin (OMST) conversion study indicated that the spiroethers specifically inhibited the OMST to AFG(1) pathway. A cytochrome P450 monooxygenase, CYPA, is known as an essential enzyme for this pathway. Because CYPA has homology with TRI4, a key enzyme catalyzing early steps in the biosynthesis of trichothecenes, the inhibitory actions of the two spiroethers against TRI4 reactions and 3-acetyldeoxynivalenol (3-ADON) production were tested. (E)- and (Z)-spiroethers inhibited the enzymatic activity of TRI4 dose-dependently and interfered with 3-ADON production by Fusarium graminearum, with IC(50) values of 27.1 and 103 microM, respectively. Our results suggest that the spiroethers inhibited AFG(1) and 3-ADON production by inhibiting CYPA and TRI4, respectively. 相似文献
180.
Tadashi Yoshinari Roger Knowles 《Biochemical and biophysical research communications》1976,69(3):705-710
Acetylene (0.1 atm) caused complete or almost complete inhibition of reduction of N2O by whole cell suspensions of Pseudomonas perfectomarinus, P. aeruginosa and Micrococcus denitrificans. Acetylene did not inhibit reduction of NO3? or NO2? by these organisms. In the presence of acetylene there was stoichiometric conversion of NO3? or NO2? to N2O with negligible subsequent reduction of the latter. In the absence of acetylene there was no or only transient accumulation of N2O. The data are consistent with the view that N2O is an obligatory intermediate in the reduction of NO2? to N2 in all of the three organisms studied. 相似文献