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41.
Yamamori T Yasui H Yamazumi M Wada Y Nakamura Y Nakamura H Inanami O 《Free radical biology & medicine》2012,53(2):260-270
Whereas ionizing radiation (Ir) instantaneously causes the formation of water radiolysis products that contain some reactive oxygen species (ROS), ROS are also suggested to be released from biological sources in irradiated cells. It is now becoming clear that these ROS generated secondarily after Ir have a variety of biological roles. Although mitochondria are assumed to be responsible for this Ir-induced ROS production, it remains to be elucidated how Ir triggers it. Therefore, we conducted this study to decipher the mechanism of Ir-induced mitochondrial ROS production. In human lung carcinoma A549 cells, Ir (10 Gy of X-rays) induced a time-dependent increase in the mitochondrial ROS level. Ir also increased mitochondrial membrane potential, mitochondrial respiration, and mitochondrial ATP production, suggesting upregulation of the mitochondrial electron transport chain (ETC) function after Ir. Although we found that Ir slightly enhanced mitochondrial ETC complex II activity, the complex II inhibitor 3-nitropropionic acid failed to reduce Ir-induced mitochondrial ROS production. Meanwhile, we observed that the mitochondrial mass and mitochondrial DNA level were upregulated after Ir, indicating that Ir increased the mitochondrial content of the cell. Because irradiated cells are known to undergo cell cycle arrest under control of the checkpoint mechanisms, we examined the relationships between cell cycle and mitochondrial content and cellular oxidative stress level. We found that the cells in the G2/M phase had a higher mitochondrial content and cellular oxidative stress level than cells in the G1 or S phase, regardless of whether the cells were irradiated. We also found that Ir-induced accumulation of the cells in the G2/M phase led to an increase in cells with a high mitochondrial content and cellular oxidative stress level. This suggested that Ir upregulated mitochondrial ETC function and mitochondrial content, resulting in mitochondrial ROS production, and that Ir-induced G2/M arrest contributed to the increase in the mitochondrial ROS level by accumulating cells in the G2/M phase. 相似文献
42.
Nishijima K Shukunami K Yoshinari H Takahashi J Maeda H Takagi H Kotsuji F 《American journal of physiology. Lung cellular and molecular physiology》2012,303(3):L208-L214
Although vernix caseosa is known to be a natural biofilm at birth, human pulmonary surfactant commences to remove the vernix from fetal skin into the amniotic fluid at gestational week 34, i.e., well before delivery. To explain this paradox, we first produced two types of fluorescently labeled liposomes displaying morphology similar to that of pulmonary surfactant and vernix caseosa complexes. We then continuously administered these liposomes into the amniotic fluid space of pregnant rabbits. In addition, we produced pulmonary surfactant and vernix caseosa complexes and administered them into the amniotic fluid space of pregnant rabbits. The intra-amniotic infused fluorescently labeled liposomes were absorbed into the fetal intestinal epithelium. However, the liposomes were not transported to the livers of fetal rabbits. We also revealed that continuous administration of micelles derived from pulmonary surfactants and vernix caseosa protected the small intestine of the rabbit fetus from damage due to surgical intervention. Our results indicate that pulmonary surfactant and vernix caseosa complexes in swallowed amniotic fluid might locally influence fetal intestinal enterocytes. Although the present studies are primarily observational and further studies are needed, our findings elucidate the physiological interactions among pulmonary, dermal-epidermal, and gastrointestinal developmental processes. 相似文献
43.
A peculiar inward growth, named a “cell wall sac”, formed in mulberry (Morus alba) idioblasts, is a subcellular site for production of calcium carbonate crystals. On the basis of ultrastructural observations,
a fully expanded cell wall sac could be divided into two parts—an amorphous complex consisting of multi-layered compartments
with multiple fibers originating from the innermost cell wall layer, and a peripheral plain matrix with fiber aggregates.
Immunofluorescent localization showed that low and highly esterified pectin epitopes were detected at the early stages of
development of the cell wall sac, followed by complete disappearance from the both parts of fully enlarged mature sac. In
contrast, the xyloglucan epitope remained in the compartment complex; this was supported by the observation that the xyloglucan
epitope labeled with immuno-gold particles is found on fibers in the complex part. 相似文献
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46.
Yoshinari Maeda Kiyoshi Yoshimura Hiroto Matsui Yoshitaro Shindo Takao Tamesa Yukio Tokumitsu Noriaki Hashimoto Yoshihiro Tokuhisa Kazuhiko Sakamoto Kouhei Sakai Yutaka Suehiro Yuji Hinoda Koji Tamada Shigefumi Yoshino Shoichi Hazama Masaaki Oka 《Cancer immunology, immunotherapy : CII》2015,64(8):1047-1056
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48.
Lack of ACTPK1, an STY kinase,enhances ammonium uptake and use,and promotes growth of rice seedlings under sufficient external ammonium 总被引:2,自引:0,他引:2
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50.
The expression of glycosylphosphatidylinositol (GPI-anchored) carcinoembryonic antigen (CEA) and alkaline phosphatase (ALP)
on the cell surface of various cancer cell lines and a lung diploid cell line (WI38) was investigated, with exposure of the
cell lines to a cell differentiation agent (sodium butyrate) to induce cell differentiation and expression of the two tumor-associated
antigens. In three colon (SW1222, SW1116, and HT-29) and stomach (MKN-45) cancer cell lines, all of which are double producers
of CEA and ALP, the maximum expression of GPI-anchored CEA occurred with butyrate at a lower concentration than did that of
GPI-anchored ALP. GPI-anchored ALP derived from colon (SW1222 and SW1116) and stomach (MKN-45 and MKN-1) cancer cell lines
was heat-stable with and without exposure to butyrate, but GPI-anchored ALP derived from lung cancer cell lines (PC-6, PC13,
PC-14, WI26VA4, and WI38VA13) showed a variety of heat stabilities, depending on cell line, butyrate exposure, and SV40 transformation.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献