全文获取类型
收费全文 | 1520篇 |
免费 | 87篇 |
出版年
2022年 | 7篇 |
2021年 | 17篇 |
2020年 | 5篇 |
2019年 | 11篇 |
2018年 | 14篇 |
2017年 | 20篇 |
2016年 | 19篇 |
2015年 | 30篇 |
2014年 | 40篇 |
2013年 | 89篇 |
2012年 | 89篇 |
2011年 | 93篇 |
2010年 | 60篇 |
2009年 | 51篇 |
2008年 | 66篇 |
2007年 | 58篇 |
2006年 | 69篇 |
2005年 | 84篇 |
2004年 | 82篇 |
2003年 | 79篇 |
2002年 | 76篇 |
2001年 | 45篇 |
2000年 | 26篇 |
1999年 | 39篇 |
1998年 | 19篇 |
1997年 | 18篇 |
1996年 | 24篇 |
1995年 | 30篇 |
1994年 | 17篇 |
1993年 | 21篇 |
1992年 | 31篇 |
1991年 | 31篇 |
1990年 | 24篇 |
1989年 | 19篇 |
1988年 | 23篇 |
1987年 | 15篇 |
1986年 | 9篇 |
1985年 | 18篇 |
1984年 | 18篇 |
1983年 | 16篇 |
1982年 | 14篇 |
1981年 | 23篇 |
1980年 | 15篇 |
1978年 | 5篇 |
1977年 | 16篇 |
1976年 | 12篇 |
1975年 | 3篇 |
1973年 | 3篇 |
1970年 | 3篇 |
1968年 | 2篇 |
排序方式: 共有1607条查询结果,搜索用时 31 毫秒
961.
962.
Ryuichiro Kurane Tomoo Suzuki Yoshimasa Takahara 《Bioscience, biotechnology, and biochemistry》2013,77(11):2119-2123
Many microorganisms (17 strains of gram-positive bacteria, 8 strains of gram-negative bacteria, 2 strains of fungi) capable of assimilating di-2-ethyl hexyl phthalate (DEHP) were isolated from soil and other natural sources. When Pseudomonas acidovorans 256–1 among these microorganisms was aerobically cultured in media containing 0.5 % of DEHP, DEHP disappeared completely in 72 hr when assayed gaschromatographically. Most of phthalate esters could be assimilated, regardless of their side-chain types. In addition, branched-alkyl phthalate was assimilated better than n-alkyl phthalate. Based on degradation rate of n-alkyl phthalate in relation to its side chain and carbon number, two peaks were observed in n-alkyl phthalate with four and seven carbon number on its side-chain. 相似文献
963.
964.
965.
Matsunaga Akira; Nomura Yasutomo; Kuroda Satoshi; Tamura Mamoru; Nishihira Jun; Yoshimura Nozomu 《American journal of physiology. Cell physiology》1998,275(4):C1022
Using theblood-free perfused rat brain, we examined the redox behavior ofcytochrome oxidase of two chromophores, hemea + a3 and copper.When perfusate inflow was stopped to induce global ischemia,the reduction of heme a + a3 was triphasic,with a rapid phase, a slow phase, and a second rapid phase. Incontrast, the reduction of copper was monophasic after the rapid phaseof heme a + a3. The triphasicreduction of heme a + a3 was diminished by energy-depleting treatments, such as addition of an uncoupler. Thetime course of the reduction of copper was not affected by the energydepletion. During global ischemia the decrease in creatine phosphate nearly paralleled the reduction of hemea + a3, whereas ATPremained at the control level until ~60% of hemea + a3 was reduced inthe rapid phase. In the slow phase, ATP started to decrease with thereduction of copper. The redox behavior of copper was similar to theslow phase of the reduction of heme a + a3 because ofthe higher oxygen affinity of copper than of hemea + a3. Therefore,the rapid phase of the reduction of hemea + a3 can be used asan alarm before a decrease in ATP, whereas the reduction of copperindicates a decrease in ATP under severe hypoxia. Thus the coppersignal in noninvasive near-infrared spectroscopy is a useful parameterfor the clinical setting. 相似文献
966.
Tadashi Matsunaga Atsushi Arakaki Mikako Takahoko 《Biotechnology and bioengineering》2002,77(6):614-618
MagA is an iron-translocating protein found in the membranes of magnetic bacterium. Luciferase-bacterial magnetic particle (BMP) complexes were prepared by artificially inserting MagA-luciferase fusion proteins into the membranes of BMPs from Magnetospirillum magneticum strain AMB-1. Fusion proteins were from recombinant Escherichia coli membranes. MagA-Luc fusion proteins were integrated by sonication in vitro. Successful integration of fusion proteins was confirmed by luciferase luminescence on BMPs. Maximum luminescence was obtained after sonication for 3 min with a solution containing 300 mM NaCl, and is 18 times higher compared with recombinant Luc-BMPs generated using previously reported gene fusion techniques. 相似文献
967.
968.
Goto T Kim YI Funakoshi K Teraminami A Uemura T Hirai S Lee JY Makishima M Nakata R Inoue H Senju H Matsunaga M Horio F Takahashi N Kawada T 《American journal of physiology. Endocrinology and metabolism》2011,301(5):E1022-E1032
Peroxisome proliferator-activated receptors (PPARs) control energy homeostasis. In this study, we showed that farnesol, a naturally occurring ligand of PPARs, could ameliorate metabolic diseases. Obese KK-Ay mice fed a high-fat diet (HFD) containing 0.5% farnesol showed significantly decreased serum glucose level, glucosuria incidence, and hepatic triglyceride contents. Farnesol-containing HFD upregulated the mRNA expressions of PPARα target genes involved in fatty acid oxidation in the liver. On the other hand, farnesol was not effective in upregulating the mRNA expressions of PPARγ target genes in white adipose tissues. Experiments using PPARα-deficient [(-/-)] mice revealed that the upregulation of fatty acid oxidation-related genes required PPARα function, but the suppression of hepatic triglyceride accumulation was partially PPARα-dependent. In hepatocytes isolated from the wild-type and PPARα (-/-) mice, farnesol suppressed triglyceride synthesis. In luciferase assay, farnesol activated both PPARα and the farnesoid X receptor (FXR) at similar concentrations. Moreover, farnesol increased the mRNA expression level of a small heterodimer partner known as one of the FXR target genes and decreased those of sterol regulatory element-binding protein-1c and fatty acid synthase in both the wild-type and PPARα (-/-) hepatocytes. These findings suggest that farnesol could improve metabolic abnormalities in mice via both PPARα-dependent and -independent pathways and that the activation of FXR by farnesol might contribute partially to the PPARα-independent hepatic triglyceride content-lowering effect. To our knowledge, this is the first study on the effect of the dual activators of PPARα and FXR on obesity-induced metabolic disorders. 相似文献
969.
970.
Watanabe T Shinya K Watanabe S Imai M Hatta M Li C Wolter BF Neumann G Hanson A Ozawa M Yamada S Imai H Sakabe S Takano R Iwatsuki-Horimoto K Kiso M Ito M Fukuyama S Kawakami E Gorai T Simmons HA Schenkman D Brunner K Capuano SV Weinfurter JT Nishio W Maniwa Y Igarashi T Makino A Travanty EA Wang J Kilander A Dudman SG Suresh M Mason RJ Hungnes O Friedrich TC Kawaoka Y 《Journal of virology》2011,85(24):13195-13203
The first influenza pandemic of the 21st century was caused by novel H1N1 viruses that emerged in early 2009. An Asp-to-Gly change at position 222 of the receptor-binding protein hemagglutinin (HA) correlates with more-severe infections in humans. The amino acid at position 222 of HA contributes to receptor-binding specificity with Asp (typically found in human influenza viruses) and Gly (typically found in avian and classic H1N1 swine influenza viruses), conferring binding to human- and avian-type receptors, respectively. Here, we asked whether binding to avian-type receptors enhances influenza virus pathogenicity. We tested two 2009 pandemic H1N1 viruses possessing HA-222G (isolated from severe cases) and two viruses that possessed HA-222D. In glycan arrays, viruses possessing HA-222D preferentially bound to human-type receptors, while those encoding HA-222G bound to both avian- and human-type receptors. This difference in receptor binding correlated with efficient infection of viruses possessing HA-222G, compared to those possessing HA-222D, in human lung tissue, including alveolar type II pneumocytes, which express avian-type receptors. In a nonhuman primate model, infection with one of the viruses possessing HA-222G caused lung damage more severe than did infection with a virus encoding HA-222D, although these pathological differences were not observed for the other virus pair with either HA-222G or HA-222D. These data demonstrate that the acquisition of avian-type receptor-binding specificity may result in more-efficient infection of human alveolar type II pneumocytes and thus more-severe lung damage. Collectively, these findings suggest a new mechanism by which influenza viruses may become more pathogenic in mammals, including humans. 相似文献