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11.
H Yoshikawa T Kukita K Kurisu H Tashiro 《Journal of craniofacial genetics and developmental biology》1987,7(1):45-51
To clarify the mechanism by which retinoid causes cleft palate, we investigated the effect of retinoic acid (RA) on proliferation activity and glycosaminoglycan (GAG) synthesis in mouse fetuses palatal mesenchymal (MFPM) cells. MFPM cells were incubated for 1-11 days with various concentrations of RA to examine its effect on growth rate. Also, confluent cultures were incubated with [3H]glucosamine or [35S]sulfate in the presence of various concentrations of RA to investigate the effect of RA on GAG synthesis. RA remarkably inhibited the growth of MFPM cells in a dose-dependent manner. RA also inhibited the synthesis of GAGs, with sulfated GAGs being more severely affected than hyaluronic acid. These data suggest that the inhibition of proliferation activity and GAG synthesis of palatal mesenchymal cells might be involved in the induction of cleft palate by retinoic acid. 相似文献
12.
Takashi Yutsudo Hisao Kurazono Chihiro Sasakawa Masanosuke Yoshikawa Makoto Iwaya Tae Takeda Yoshifumi Takeda 《FEMS microbiology letters》1987,48(1-2):273-276
Abstract A Vero toxin (VT2 or Shiga-like toxin II)-converting phage was isolated from Escherichia coli 0157: H7 strain J-2. Nontoxigenic E. coli C600 produced VT2 when lysogenized with the toxin-converting phage. Eco RI fragments of the phage DNA were ligated with Eco RI-digested pBR322 or pUC118 and were transformed into E. coli MC1061 or MV1184. Transformants exhibiting VT2 production commonly contained a 4.6 kb Eco RI fragment. It was found that a 2.3 kb Kpn I- Sph I fragment coded VT2 production and that this fragment hybridized weakly with the 2.1 kb fragment encoding VT1. 相似文献
13.
Yasushi Harada 《Journal of Ethology》1989,7(1):17-25
A theory on the evolution of human primary sex ratio is proposed. Effects of parental preference for sons, reflected in birth
control based on offspring sex ratio and female biased infanticide, on the evolution of primary sex ratio are analyzed. Both
are shown to select for female bias in primary sex ratio. The gene-culture coevolution of female infanticide and primary sex
ratio is also studied and it is shown that female infanticide develops more in societies in which the father plays a more
important role in the transmission of culture than the mother does. 相似文献
14.
15.
T Yoshikawa W Flory D H Giamalva L P Ruhr D F Church W A Pryor 《Biochemistry international》1988,16(5):929-934
Male Sprague-Dawley rats were pretreated i.p. with corn oil or DMSO (1.5 ml/kg/day), or with beta-naphthoflavone (BNF, 40 mg/kg/day) in corn oil or DMSO for 3 days. 1-Nitropyrene (1-NP, 150 mg/kg) in DMSO was injected i.p. 24 hr after pretreatment. A significant increase in the levels of several serum enzymes was seen in rats pretreated with corn oil alone 24 hr after 1-NP injection. The increase in enzyme activities was significantly reduced by a 3-day pretreatment with DMSO or BNF. 相似文献
16.
Isamu Tanaka Akio Horie Joji Haratake Yasushi Kodama Kenzaburo Tsuchiya 《Biological trace element research》1988,16(1):19-26
There are few inhalation studies of nickel carcinogenesis. In this study, Wistar male rats were exposed to green nickel oxide
(NiO(G)) aerosols (mass median aerodynamic diameter, 0.6 μm) for 7 h/d, 5 d/wk for up to 12 mo. The average exposure concentration
was controlled at 0.3 and 1.2 mg/m3 during the exposure. For histopathological examination and measurement of the nickel concentration in rat organs, the rats
were sacrificed at 3, 6, and 12 mo of exposure and 8 mo clearance period following 12 mo of exposure.
The nickel content in rat lungs that was observed up to 2.6 mg after 12 mo exposure, was proportional to the exposure concentration
during the exposure. The clearance of the nickel from the lungs was very slow and the biological half time was determined
7.7 mo.
Although the rats were exposed continuously to NiO(G), for 12 mo and kept for 8 mo clearance period, there were no malignant
tumors in any of the exposed animals. 相似文献
17.
18.
Cloning and CO2 -dependent expression of the genetic region for encapsulation from Bacillus anthracis 总被引:7,自引:1,他引:6
The capsule of Bacillus anthracis is an important virulence factor consisting of poly-D-glutamic acid. The genetic region required for the encapsulation was cloned in Escherichia coli from the capsule plasmid pTE702, using a selection procedure based on an immunodiffusion assay. The cloned region directed synthesis of the capsule both in E. coli and B. anthracis. Capsule synthesis from these clones, as in the wild type, was dependent upon the presence of CO2. However, encapsulation directed by the cloned fragment was less marked than from pTE702. Another region enhancing capsulation was shown to exist on pTE702. The minimum size of the encapsulation region was defined to within 2.7 kb DNA and shown to be essential for the encapsulation in B. anthracis. 相似文献
19.
Masaki Fujimura Yasushi Miyake Kohhei Uotani Kazunori Kanamori Tamotsu Matsuda 《Prostaglandins & other lipid mediators》1988,35(3)
Effect of a thromboxane synthetase inhibitor (OKY-046) on bronchoconstriction induced by aerosol leukotriene C4 and histamine was studied in anesthetized, artificially ventilated guinea pigs in order to examine whether secondary release of thromboxane A2 is produced by aerosol leukotriene C4 or not. 0.01–1.0μg/ml of leukotriene C4 and 12.5–400μg/ml of histamine inhaled from ultrasonic nebulizer developed for small animals caused dose-dependent increase of pressure at airway opening (Pao) which is considered to be an index representing bronchial response. Pretreatment of the animals with intravenous OKY-046 (100mg/kg) significantly reduced the airway responses produced by inhalation of 0.1, 0.33 and 1.0μg/ml of leukotriene C4, while the pretreatment did not affect the histamine dose-response curve. Based on these findings and previous reports (6, 7), it is suggested that aerosol leukotriene C4 activates arachidonate cyclooxygenase pathway including thromboxane A2 synthesis and the released cyclooxygenase products have bronchodilating effect as a whole 相似文献
20.