全文获取类型
收费全文 | 445篇 |
免费 | 32篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 6篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 15篇 |
2014年 | 11篇 |
2013年 | 30篇 |
2012年 | 21篇 |
2011年 | 30篇 |
2010年 | 10篇 |
2009年 | 29篇 |
2008年 | 27篇 |
2007年 | 34篇 |
2006年 | 44篇 |
2005年 | 34篇 |
2004年 | 45篇 |
2003年 | 28篇 |
2002年 | 42篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 9篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 7篇 |
1994年 | 6篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有477条查询结果,搜索用时 203 毫秒
91.
Since very few previous studies have carried out the quantitative analysis for the colocalization of nitric oxide (NO) and vasoactive intestinal peptide (VIP) in the submucous neurons in the rat digestive tract, we applied in vivo treatment of colchicine to enhance the immunoreactivity and examined the colocalization of NO synthase (nNOS) and VIP in neurons of the submucous plexus throughout the rat digestive tract. The density of nNOS-containing neurons in the submucous plexus in the stomach corpus (103±25 cells/cm2, n=3) and that in the antrum (157±9 cells/cm2, n=3) were significantly lower than those in small and large intestine. However no difference was detected in the cell density among duodenum (1967±188 cells/cm2, n=3), jejunum (2640±140 cells/cm2, n=3), ileum (2070±42 cells/cm2, n=3), proximal colon (2243±138 cells/cm2, n=3) and distal colon (2633±376 cells/cm2, n=3). The proportion of nNOS-immunoreactive (IR), nNOS/VIP-IR and VIP-IR neurons to the total number of submucous neurons was examined. nNOS/VIP-IR neurons comprised 45–55% of total number of submucous neurons from the duodenum to the proximal colon, however those comprised 66.4±5.1% in the distal colon. The results showed that the dense distribution of nNOS-containing neurons was found in the submucous plexus throughout the small and large intestine, and large population of submucous neurons co-stored nNOS and VIP. 相似文献
92.
93.
T2BP, a novel TRAF2 binding protein, can activate NF-kappaB and AP-1 without TNF stimulation. 总被引:2,自引:0,他引:2
Mutsumi Kanamori Harukazu Suzuki Rintaro Saito Masami Muramatsu Yoshihide Hayashizaki 《Biochemical and biophysical research communications》2002,290(3):1108-1113
TRAF2 is a key molecule involved in TNF signaling, which is crucial for the regulation of inflammatory processes. We have identified a novel TRAF2 binding protein, designated as T2BP (TRAF2 binding protein), by a mammalian two-hybrid screening approach. T2BP is a relatively small protein of 184 amino acids, which includes a forkhead-associated domain, the phosphopeptide binding motif. The interaction domain search showed that the TRAF domain in TRAF2 is required for the binding to T2BP whereas almost the entire protein in T2BP binds to TRAF2. The interaction was further confirmed by co-immunoprecipitation. Expression profiling for T2BP and TRAF2 revealed an ubiquitous expression in adult mouse tissues. Overexpression of T2BP in HEK293 cells activated NF-kappaB and AP-1 in a dose dependent manner as well as seen in the TNF-treated control cells. Our results suggest that T2BP is involved in the TNF-mediated signaling by its interaction with TRAF2. 相似文献
94.
Taku Wakabayashi Hisamichi Naito Jun-ichi Suehiro Yang Lin Hideya Kawaji Tomohiro Iba Tsukasa Kouno Sachi Ishikawa-Kato Masaaki Furuno Kazuhiro Takara Fumitaka Muramatsu Jia Weizhen Hiroyasu Kidoya Katsuhiko Ishihara Yoshihide Hayashizaki Kohji Nishida Mervin C. Yoder Nobuyuki Takakura 《Cell Stem Cell》2018,22(3):384-397.e6
95.
Reproducible Alterations of DNA Methylation at a Specific Population of CpG Islands during Blast Formation of Peripheral Blood Lymphocytes 总被引:1,自引:0,他引:1
Kuromitsu Junro; Kataoka Hiroshi; Yamashita Hideji; Muramatsu Masami; Furuichi Yasuhiro; Sekine Teruaki; Hayashizaki Yoshihide 《DNA research》1995,2(6):263-267
We investigated the changes in the methylation patterns of CpGislands associated with blast formation of human peripheralblood lymphocytes activated by anti-CD3 and interleukin-2 (IL-2),using restriction landmark genomic scanning with a methylation-sensitiverestriction enzyme (RLGS-M) system. Of about 2,100 Not I spot/lociwhich were analyzed, only 10 showed changes, whereas drasticchanges have been observed in cases of malignant and SV40 transformation.These changes were highly reproducible for samples from boththe same and different individuals. Even the timing of the changesafter cultivation was the same. Thus, we concluded that at leastthe genomic DNA methylation state in vivo was essentially retainedin T blast cells activated in vitro by induction with IL-2 andanti-CD3, which are commonly used in biological experimentsas well as clinical diagnosis and therapy. 相似文献
96.
High Efficiency Selection of Full-length cDNA by Improved Biotinylated Cap Trapper 总被引:15,自引:0,他引:15
Carninci Piero; Westover Arthur; Nishiyama Yoko; Ohsumi Tomoya; Itoh Masayoshi; Nagaoka Sumiharu; Sasaki Nobuya; Okazaki Yasushi; Muramatsu Masami; Schneider Claudio; Hayashizaki Yoshihide 《DNA research》1997,4(1):61-66
We report here an improved protocol for the preparation of full-lengthcDNA libraries that improves the previously reported method(Carninci, P., Kvam, K., Kitamura, A. et al. 1996, Genomics,137, 327336), that allows long cDNAs to be cloned moreefficiently. One potential disadvantage of the original biotinylatedCAP trapper protocol is the exposure of mRNA to chemical andenzymatic attacks during the biotinylation of the cap structure,before the first-strand cDNA synthesis (and selection of full-lengthcDNA by biotinylated cap). Here, we show that the biotinylationof the cap structure is very specific and effective even ifbiotinylation is performed on the mRNA/cDNA hybrid producedby the first-strand cDNA synthesis reaction. Consequently, mRNAremains protected from chemical and enzymatic degradation duringthe overnight biotinylation step, thus making it possible toselect full-length cDNAs of longer average size. We herein reportthe efficiency and specificity of the new version of the protocolfor cap structure biotinylation and capture of full-length cDNA. 相似文献
97.
Shinji Yamamoto Yoshihide Shiraki Shintaro Uehara Keisuke Kushiro 《Somatosensory & motor research》2016,33(2):130-136
In the present study, we investigated the kinematics of object-transport movement in a downward direction using a precision grip, to elucidate how the central nervous system (CNS) takes into account object weight when making the movement, even when participants are unable to recognize the weight until they grasp the object. We found that the kinematics during transport movement were significantly changed by the object weight, even when the weight was unrecognized visually, suggesting that the CNS controls object-transport movement in a downward direction according to object weight, regardless of the visual recognizability of the weight. 相似文献
98.
Toku Kanaseki Takeshi Miyatsuka Yoshio Fujitani Hirotaka Watada Yoshihide Tsujimoto Shigeomi Shimizu 《The EMBO journal》2016,35(18):1991-2007
Autophagy is a cellular process that degrades subcellular constituents, and is conserved from yeast to mammals. Although autophagy is believed to be essential for living cells, cells lacking Atg5 or Atg7 are healthy, suggesting that a non‐canonical degradation pathway exists to compensate for the lack of autophagy. In this study, we show that the budding yeast Saccharomyces cerevisiae, which lacks Atg5, undergoes bulk protein degradation using Golgi‐mediated structures to compensate for autophagy when treated with amphotericin B1, a polyene antifungal drug. We named this mechanism Golgi membrane‐associated degradation (GOMED) pathway. This process is driven by the disruption of PI(4)P‐dependent anterograde trafficking from the Golgi, and it also exists in Atg5‐deficient mammalian cells. Biologically, when an Atg5‐deficient β‐cell line and Atg7‐deficient β‐cells were cultured in glucose‐deprived medium, a disruption in the secretion of insulin granules from the Golgi occurred, and GOMED was induced to digest these (pro)insulin granules. In conclusion, GOMED is activated by the disruption of PI(4)P‐dependent anterograde trafficking in autophagy‐deficient yeast and mammalian cells. 相似文献
99.
The present study was conducted to describe neonates' vocalizations and facial expressions in crying states, and to examine
the effects of intra-uterine sounds on neonates' vocal and facial behaviors. Based on 22 h taped data taken from 4 healthy
full-term neonates ranging in ages of 10–168 h, a total of 1020 samples of vocalization and their accompanying facial expressions
were analyzed, and 12 categories of vocalizations and 8 categories of facial expressions were classified. In the playback
experiments, it was shown that presentation of intra-uterine sounds changed the fundamental frequencies of infant cry vocalizations.
Furthermore, it was found that the intra-uterine sounds elicited a progenitor of the facial expression of “affection” which
mainly occurred accompanying the emission of short and low vocalizations. 相似文献
100.
Yoshinori Kariya Kikuya Kato Yoshihide Hayashizaki Seiichi Himeno Seiichiro Tarui Kenichi Matsubara 《Gene》1986,50(1-3):345-352