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81.
Hayashizaki Y 《Comptes rendus biologies》2003,326(10-11):923-929
The Riken mouse genome encyclopedia a comprehensive full-length cDNA collection and sequence database. High-level functional annotation is based on sequence homology search, expression profiling, mapping and protein-protein interactions. More than 1000000 clones prepared from 163 tissues were end-sequenced and classified into 128000 clusters, and 60000 representative clones were fully sequenced representing 24000 clear protein-encoding genes. The application of the mouse genome database for positional cloning and gene network regulation analysis is reported. 相似文献
82.
Atsutaka Kubosaki Yasuhiro Tomaru Michihira Tagami Erik Arner Hisashi Miura Takahiro Suzuki Masanori Suzuki Harukazu Suzuki Yoshihide Hayashizaki 《Genome biology》2009,10(4):R41-14
Background
Immediate early genes are considered to play important roles in dynamic gene regulatory networks following exposure to appropriate stimuli. One of the immediate early genes, early growth response gene 1 (EGR-1), has been implicated in differentiation of human monoblastoma cells along the monocytic commitment following treatment with phorbol ester. EGR-1 has been thought to work as a modifier of monopoiesis, but the precise function of EGR-1 in monocytic differentiation has not been fully elucidated. 相似文献83.
84.
Kawakami E Kinouchi N Adachi T Ohsawa Y Ishimaru N Ohuchi H Sunada Y Hayashi Y Tanaka E Noji S 《Development, growth & differentiation》2011,53(1):48-54
Small interfering RNA (siRNA)-mediated silencing of gene expression is rapidly becoming a powerful tool for molecular therapy. However, the rapid degradation of siRNAs and their limited duration of activity require efficient delivery methods. Atelocollagen (ATCOL)-mediated administration of siRNAs is a promising approach to disease treatment, including muscular atrophy. Herein, we report that ATCOL-mediated systemic administration of a myostatin-targeting siRNA into a caveolin-3-deficient mouse model of limb-girdle muscular dystrophy 1C (LGMD1C) induced a marked increase in muscle mass and a significant recovery of contractile force. These results provide evidence that ATCOL-mediated systemic administration of siRNAs may be a powerful therapeutic tool for disease treatment, including muscular atrophy. 相似文献
85.
Since very few previous studies have carried out the quantitative analysis for the colocalization of nitric oxide (NO) and vasoactive intestinal peptide (VIP) in the submucous neurons in the rat digestive tract, we applied in vivo treatment of colchicine to enhance the immunoreactivity and examined the colocalization of NO synthase (nNOS) and VIP in neurons of the submucous plexus throughout the rat digestive tract. The density of nNOS-containing neurons in the submucous plexus in the stomach corpus (103±25 cells/cm2, n=3) and that in the antrum (157±9 cells/cm2, n=3) were significantly lower than those in small and large intestine. However no difference was detected in the cell density among duodenum (1967±188 cells/cm2, n=3), jejunum (2640±140 cells/cm2, n=3), ileum (2070±42 cells/cm2, n=3), proximal colon (2243±138 cells/cm2, n=3) and distal colon (2633±376 cells/cm2, n=3). The proportion of nNOS-immunoreactive (IR), nNOS/VIP-IR and VIP-IR neurons to the total number of submucous neurons was examined. nNOS/VIP-IR neurons comprised 45–55% of total number of submucous neurons from the duodenum to the proximal colon, however those comprised 66.4±5.1% in the distal colon. The results showed that the dense distribution of nNOS-containing neurons was found in the submucous plexus throughout the small and large intestine, and large population of submucous neurons co-stored nNOS and VIP. 相似文献
86.
87.
88.
Yoshihide Yamasaki Osamu Shimamura Akira Kizu Masao Nakagawa Hamao Ijichi 《Life sciences》1982,31(5):471-478
The formaldehyde method was used to examine the interaction of PGE1 with morphine, β-endorphin and Met-enkephalin on rat mast cells by their effects on IgE-mediated 14C-serotonin release. PGE1 (2×10?8?2×10?5 M) caused a dose-related inhibition of the mediator release 1 min after an antigen challenge, and morphine (3×10?7?3×10?5 M) reversed this PGE1 effect dose-dependently and stereospecifically; naloxone (2×10?4 M) antagonized this action of morphine. β-Endorphin (3×10?7?10?5 M) and Met-enkephalin (3×10?6?10?4 M) mimicked this morphine action dose-dependently and were antagonized by naloxone (2×10?4 M). These results suggest that morphine and endorphins modulate immunological mediator release from rat mast cells through opioid receptors. 相似文献
89.
Hata K Andoh A Shimada M Fujino S Bamba S Araki Y Okuno T Fujiyama Y Bamba T 《American journal of physiology. Gastrointestinal and liver physiology》2002,282(6):G1035-G1044
Colonic subepithelial myofibroblasts (SEMFs) may play a role in the modulation of mucosal inflammatory responses. We investigated the effects of interleukin (IL)-17 on IL-6 and chemokine [IL-8 and monocyte chemoattractant protein (MCP)-1] secretion in colonic SEMFs. Cytokine expression was determined by ELISA and Northern blotting. Nuclear factor kappa B (NF-kappaB) DNA-binding activity was evaluated by electrophortetic gel mobility shift assay (EMSA). The activation of mitogen-activated protein kinase (MAPK) was assessed by immunoblotting. IL-6, IL-8, and MCP-1 secretions were rapidly induced by IL-17. IL-17 induced NF-kappaB activation within 45 min after stimulation. A blockade of NF-kappaB activation markedly reduced these responses. MAPK inhibitors (SB-203580, PD-98059, and U-0126) significantly reduced the IL-17-induced IL-6 and chemokine secretion. The combination of either IL-17 + IL-1beta or IL-17 + tumor necrosis factor (TNF)-alpha enhanced cytokine secretion; in particular, the effects of IL-17 + TNF-alpha on IL-6 secretion were much stronger than the other responses. This was dependent on the enhancement of IL-6 mRNA stability. In conclusion, human SEMFs secreted IL-6, IL-8, and MCP-1 in response to IL-17. These responses might play an important role in the pathogenesis of gut inflammation. 相似文献
90.
Shinji Kondo Akira Shinagawa Tetsuya Saito Hidenori Kiyosawa Itaru Yamanaka Katsunori Aizawa Shiro Fukuda Ayako Hara Masayoshi Itoh Jun Kawai Kazuhiro Shibata Yoshihide Hayashizaki 《Mammalian genome》2001,12(9):673-677
Although the sequencing of the human genome is complete, identification of encoded genes and determination of their structures
remain a major challenge. In this report, we introduce a method that effectively uses full-length mouse cDNAs to complement
efforts in carrying out these difficult tasks. A total of 61,227 RIKEN mouse cDNAs (21,076 full-length and 40,151 EST sequences
containing certain redundancies) were aligned with the draft human sequences. We found 35,141 non-redundant genomic regions
that showed a significant alignment with the mouse cDNAs. We analyzed the structures and compositional properties of the regions
detected by the full-length cDNAs, including cross-species comparisons, and noted a systematic bias of GENSCAN against exons
of small size and/or low GC-content. Of the cDNAs locating the 35,141 genomic regions, 3,217 did not match any sequences of
the known human genes or ESTs. Among those 3,217 cDNAs, 1,141 did not show any significant similarity to any protein sequence
in the GenBank non-redundant protein database and thus are candidates for novel genes.
Received: 18 January 2001 / Accepted: 17 May 2001 相似文献