全文获取类型
收费全文 | 494篇 |
免费 | 42篇 |
专业分类
536篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 6篇 |
2017年 | 2篇 |
2016年 | 8篇 |
2015年 | 15篇 |
2014年 | 11篇 |
2013年 | 31篇 |
2012年 | 21篇 |
2011年 | 31篇 |
2010年 | 11篇 |
2009年 | 31篇 |
2008年 | 31篇 |
2007年 | 37篇 |
2006年 | 47篇 |
2005年 | 35篇 |
2004年 | 46篇 |
2003年 | 28篇 |
2002年 | 48篇 |
2001年 | 12篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 10篇 |
1997年 | 9篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 10篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有536条查询结果,搜索用时 15 毫秒
21.
22.
Ohe Y Ohnishi H Okazawa H Tomizawa K Kobayashi H Okawa K Matozaki T 《Biochemical and biophysical research communications》2003,308(4):719-725
Membrane glycoproteins of neural cells play crucial roles in axon guidance, synaptogenesis, and neuronal transmission. We have here characterized membrane glycoproteins containing terminal alpha-mannose residues in rat brain membranes. Affinity purification using Galanthus nivalis agglutinin, that is highly specific for terminal alpha-mannose residues, revealed a 50-kDa protein as well as 80-kDa SHPS-1 and 45-kDa beta2 subunit of Na,K-ATPase in rat brain membranes. Combination of N-terminal peptide sequencing and mass spectrometry indicated that the 50-kDa protein was rat nucleotide pyrophosphatase-5 (NPP-5). In contrast to other NPPs, NPP-5 was a type-I transmembrane protein. Northern blot analysis showed that NPP-5 was highly expressed in brain, but also expressed in other peripheral tissues. However, we could not detect either the NPP activity or the lysophospholipase D activity in the immunoprecipitates with antibodies to NPP-5 from rat brain membranes. These data, therefore, suggest that NPP-5 is a neural oligomannosidic glycoprotein that may participate in neural cell communications. 相似文献
23.
24.
Comprehensive sequence analysis of translation termination sites in various eukaryotes 总被引:3,自引:0,他引:3
Ozawa Y Hanaoka S Saito R Washio T Nakano S Shinagawa A Itoh M Shibata K Carninci P Konno H Kawai J Hayashizaki Y Tomita M 《Gene》2002,300(1-2):79-87
Recent investigations into the translation termination sites of various organisms have revealed that not only stop codons but also sequences around stop codons have an effect on translation termination. To investigate the relationship between these sequence patterns and translation as well as its termination efficiency, we analysed the correlation between strength of consensus and translation efficiency, as predicted according to Codon Adaptation Index (CAI) value. We used RIKEN full-length mouse cDNA sequences and ten other eukaryotic UniGene datasets from NCBI for the analyses. First, we conducted sequence profile analyses following translation termination sites. We found base G and A at position +1 as a strong consensus for mouse cDNA. A similar consensus was found for other mammals, such as Homo sapiens, Rattus norvegicus and Bos taurus. However, some plants had different consensus sequences. We then analysed the correlation between the strength of consensus at each position and the codon biases of whole coding regions, using information content and CAI value. The results showed that in mouse cDNA, CAI value had a positive correlation with information content at positions +1. We also found that, for positions with strong consensus, the strength of the consensus is likely to have a positive correlation with CAI value in some other eukaryotes. Along with these observations, biological insights into the relationship between gene expression level, codon biases and consensus sequence around stop codons will be discussed. 相似文献
25.
Extra-long first-strand cDNA synthesis 总被引:5,自引:0,他引:5
26.
Nakamura N Miyake Y Matsushita M Tanaka S Inoue H Kanazawa H 《Journal of biochemistry》2002,132(3):483-491
Kinesin family proteins are microtubule-dependent molecular motors involved in the intracellular motile process. Using a Ca2+ -binding protein, CHP (calcineurin B homologous protein), as a bait for yeast two hybrid screening, we identified a novel kinesin-related protein, KIF1Bbeta2. KIF1Bbeta2 is a member of the KIF1 subfamily of kinesin-related proteins, and consists of an amino terminal KIF1B-type motor domain followed by a tail region highly similar to that of KIF1A. CHP binds to regions adjacent to the motor domains of KIF1Bbeta2 and KIF1B, but not to those of the other KIF1 family members, KIF1A and KIF1C. Immunostaining of neuronal cells showed that a significant portion of KIF1Bbeta2 is co-localized with synaptophysin, a marker protein for synaptic vesicles, but not with a mitochondria-staining dye. Subcellular fractionation analysis indicated the co-localization of KIF1Bbeta2 with synaptophysin. These results suggest that KIF1Bbeta2, a novel CHP-interacting molecular motor, mediates the transport of synaptic vesicles in neuronal cells. 相似文献
27.
Molecular analysis of gene expression in the developing pontocerebellar projection system 总被引:7,自引:0,他引:7
Díaz E Ge Y Yang YH Loh KC Serafini TA Okazaki Y Hayashizaki Y Speed TP Ngai J Scheiffele P 《Neuron》2002,36(3):417-434
As an approach toward understanding the molecular mechanisms of neuronal differentiation, we utilized DNA microarrays to elucidate global patterns of gene expression during pontocerebellar development. Through this analysis, we identified groups of genes specific to neuronal precursor cells, associated with axon outgrowth, and regulated in response to contact with synaptic target cells. In the cerebellum, we identified a phase of granule cell differentiation that is independent of interactions with other cerebellar cell types. Analysis of pontine gene expression revealed that distinct programs of gene expression, correlated with axon outgrowth and synapse formation, can be decoupled and are likely influenced by different cells in the cerebellar target environment. Our approach provides insight into the genetic programs underlying the differentiation of specific cell types in the pontocerebellar projection system. 相似文献
28.
29.
Konishi A Shimizu S Hirota J Takao T Fan Y Matsuoka Y Zhang L Yoneda Y Fujii Y Skoultchi AI Tsujimoto Y 《Cell》2003,114(6):673-688
It is poorly understood how apoptotic signals arising from DNA damage are transmitted to mitochondria, which release apoptogenic factors into the cytoplasm that activate downstream destruction programs. Here, we identify histone H1.2 as a cytochrome c-releasing factor that appears in the cytoplasm after exposure to X-ray irradiation. While all nuclear histone H1 forms are released into the cytoplasm in a p53-dependent manner after irradiation, only H1.2, but not other H1 forms, induced cytochrome c release from isolated mitochondria in a Bak-dependent manner. Reducing H1.2 expression enhanced cellular resistance to apoptosis induced by X-ray irradiation or etoposide, but not that induced by other stimuli including TNF-alpha and UV irradiation. H1.2-deficient mice exhibited increased cellular resistance in thymocytes and the small intestine to X-ray-induced apoptosis. These results indicate that histone H1.2 plays an important role in transmitting apoptotic signals from the nucleus to the mitochondria following DNA double-strand breaks. 相似文献
30.
Masayuki Mori Shingo Akiyoshi Yosuke Mizuno Hisato Okuizumi Yasushi Okazaki Yoshihide Hayashizaki Masahiko Nishimura 《Mammalian genome》1998,9(9):695-709
We have applied the restriction landmark genomic scanning (RLGS) method to the SMXA recombinant inbred (RI) mouse strain
set to reveal its detailed genetic profile. A total of 663 polymorphic RLGS spot loci were identified, 576 of which were assigned
to chromosomes. Strain distribution patterns (SDPs) at 55 microsatellite marker loci were also obtained. As a result, the
total number of loci with distinct SDPs on chromosomes increased to 400. These loci were dispersed on all chromosomes, except
for the Chromosome (Chr) Y, and effectively covered the genome with an average spacing of 4 cM. The SMXA RI strain set, hereby,
would be of value for genetic study.
Received: 20 February 1998 / Accepted: 19 May 1998 相似文献