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951.
Morphological and cytoskeletal changes caused by non-membrane damaging cytotoxin of Vibrio cholerae on Int 407 and HeLa cells 总被引:1,自引:0,他引:1
Indira Basu Rupak Mitra Pradip Kumar Saha Amar Nath Ghosh Jayanta Bhattacharya Manoj Kumar Chakrabarti Yoshifumi Takeda G.Balakrish Nair 《FEMS microbiology letters》1999,179(2):255-263
End-group mediated conjugation of bacterial polysaccharides (PSs) to carrier proteins containing T-helper cell epitopes renders such polysaccharides immunogenic also in young infants. Optimal construction of such conjugate vaccines requires fragmentation of the PS prior to the coupling reaction. In the present study a general simple and inexpensive method for the fragmentation of PSs is presented. It is based on the irradiation of isolated PSs in an electron beam accelerator. Exposure of isolated pneumococcal capsular polysaccharides (PnPSs) to ionizing radiation resulted in their partial depolymerization in a radiation dose-dependent manner. Radiation, unlike sonication, generated PnPS fragments of molecular size lower than 50 kDa and as small as 1.5 kDa when high radiation doses were used. These PnPS fragments have terminal reducing groups that can be easily used for chemical activation and subsequent coupling to any chosen carrier protein. The radiation-produced PnPS fragments retained their antigenic epitopes, when compared to native, full-size PnPSs as determined by enzyme-linked immunoassay. 相似文献
952.
We used morpholino groups to protect phosphate during the phosphorylation of the 5'-terminal ends of oligodeoxynucleotides, via phosphotriester and phosphoramidite intermediates. These groups could be removed selectively. 相似文献
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Toshio Omori Yoshifumi Jigami Yasuji Minoda 《Bioscience, biotechnology, and biochemistry》2013,77(9):1775-1779
As a result of screening isoalkyl or isoalkenyl substituted aromatic hydrocarbon assimilating microorganisms, 19 strains of isopropylbenzene assimilating bacteria were isolated. Thirteen of these strains were found to grow on α-methylstyrene and all 4 strains tested were also found to grow on isobutylbenzene.Among them, 2 strains (S107B1 and S182BI) were selected for further study and were identified as Ps. convexa and Ps. ovalis, respectively.Furthermore, some examined aromatic hydrocarbon utilizing bacteria were classified into two groups by differences in substrate assimilation specificity. 相似文献
956.
Bacteriophage P22 and Staphylococcus aureus Attenuation on Nonporous Fomites as Determined by Plate Assay and Quantitative PCR 下载免费PDF全文
Decay rates of bacteriophage P22 and Staphylococcus aureus on six types of common household inanimate surfaces were evaluated based on cultivation and quantitative PCR. A much higher level of inactivation was observed using the plate assay, suggesting that detection of the pathogen genome in samples from fomites does not necessarily imply a health risk to humans. 相似文献
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Kursat Oguz Yaykasli Toshitaka Oohashi Satoshi Hirohata Omer Faruk Hatipoglu Kiichi Inagawa Kadir Demircan Yoshifumi Ninomiya 《Molecular and cellular biochemistry》2009,323(1-2):69-79
ADAMTS9 is a member of the disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) genes, with aggrecan-degrading activity. It has also been characterized to be reactive and highly activated ADAMTS by IL-1β in both chondrosarcoma cells and human chondrocytes (Demircan et al. Arthritis Rheum 52:1451–1460, 2005). In order to understand the regulation of ADAMTS9 gene expression a functional 3.0 kb human ADAMTS9 promoter has been cloned and characterized. A sequence analysis of the promoter revealed the presence of putative binding sites for Nuclear Factor of Activated T cells (NFAT), which is commonly found in the ADAMTS4 and ADAMTS5 promoters. NFATc1 was up-regulated in an activated form by IL-1β in human chondrocytes. The IL-1β inducible ADAMTS9 expression was inhibited by NFAT inhibitors, FK506 and 11Arg (11R)-VIVIT. Furthermore, direct binding of NFATc1 on distal and proximal promoters of ADAMTS9 was demonstrated by a chromatin immunoprecipitation assay. Promoter-reporter assays supported those results. These findings may provide a better understanding of the regulation of ADAMTS9 expression induced by inflammatory cytokines. 相似文献
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