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21.
Yoshiaki Tsuda Madoka Kimura Shuri Kato Toshio Katsuki Yuzuru Mukai Yoshihiko Tsumura 《Journal of plant research》2009,122(4):367-375
The genetic resources of a particular species of flowering cherry, Cerasus jamasakura, have high conservation priority because of its cultural, ecological and economic value in Japan. Therefore, the genetic
structures of 12 natural populations of C. jamasakura were assessed using ten nuclear SSR loci. The population differentiation was relatively low (F
ST, 0.043), reflecting long-distance dispersal of seeds by animals and historical human activities. However, a neighbor-joining
tree derived from the acquired data, spatial analysis of molecular variance and STRUCTURE analysis revealed that the populations
could be divided into two groups: one located on Kyusyu Island and one on Honshu Island. Genetic diversity parameters such
as allelic richness and gene diversity were significantly lower in the Kyushu group than the Honshu group. Furthermore, STRUCTURE
analysis revealed that the two lineages were admixed in the western part of Honshu Island. Thus, although the phylogeographical
structure of the species and hybridization dynamics among related species need to be evaluated in detail using several marker
systems, the Kyusyu Island and Honshu Island populations should be considered as different conservation units, and the islands
should be regarded as distinct seed transfer zones for C. jamasakura, especially when rapid assessments are required. 相似文献
22.
Fujita N Markova D Anderson DG Chiba K Toyama Y Shapiro IM Risbud MV 《The Journal of biological chemistry》2012,287(20):16975-16986
23.
Microdissected thin perforator flaps: 46 cases 总被引:8,自引:0,他引:8
24.
Osamu Nunobiki Daisuke Sano Kyoko Akashi Taro Higashida Toshitada Ogasawara Hikari Akise Shinji Izuma Kiyo Torii Yoshiaki Okamoto Ichiro Tanaka Masatsugu Ueda 《Human cell》2016,29(2):91-95
To investigate the clinical significance of ALDH2 genetic polymorphisms in cervical carcinogenesis. ALDH2 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 195 cervical smear in exfoliated cervical cell samples using Real-Time polymerase chain reaction (PCR) System. The frequency for the AG+AA genotype was seven in the normal group (70.0 %), 16 in the LSIL group (57.1 %), and 27 in the HSIL group (90.0 %). A significant difference was found between the LSIL and HSIL groups (P = 0.0064). Patients with HSIL lesions frequently had high-risk HPV infections and concurrently belonged to the AG+AA group. ALDH2 genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population. 相似文献
25.
Nakashima A Maruki Y Imamura Y Kondo C Kawamata T Kawanishi I Takata H Matsuura A Lee KS Kikkawa U Ohsumi Y Yonezawa K Kamada Y 《PloS one》2008,3(5):e2223
The target of rapamycin (Tor) protein plays central roles in cell growth. Rapamycin inhibits cell growth and promotes cell cycle arrest at G1 (G0). However, little is known about whether Tor is involved in other stages of the cell division cycle. Here we report that the rapamycin-sensitive Tor complex 1 (TORC1) is involved in G2/M transition in S. cerevisiae. Strains carrying a temperature-sensitive allele of KOG1 (kog1-105) encoding an essential component of TORC1, as well as yeast cell treated with rapamycin show mitotic delay with prolonged G2. Overexpression of Cdc5, the yeast polo-like kinase, rescues the growth defect of kog1-105, and in turn, Cdc5 activity is attenuated in kog1-105 cells. The TORC1-Type2A phosphatase pathway mediates nucleocytoplasmic transport of Cdc5, which is prerequisite for its proper localization and function. The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation. Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation. 相似文献
26.
Sato Tomonori Ito Yoshiaki Nagasawa Takashi 《Molecular and cellular biochemistry》2015,402(1-2):93-100
Molecular and Cellular Biochemistry - Muscle wasting impairs physical function and leads people to a bedridden state. We previously demonstrated that lysine (Lys) suppresses autophagic-proteolysis... 相似文献
27.
Hitomi Y Watanabe S Kizaki T Sakurai T Takemasa T Haga S Ookawara T Suzuki K Ohno H 《Redox report : communications in free radical research》2008,13(5):213-216
Exercise dramatically increases oxygen consumption and causes oxidative stress. Superoxide dismutase (SOD) is important in the first-line defence mechanisms against oxidative stress. To investigate the effect of acute exercise on the expression of SOD, we examined the expression of mRNA for three SOD isozymes, in mice run on a treadmill to exhaustion. Six hours after exercise, the expression of extracellular SOD (EC-SOD) mRNA increased significantly in skeletal muscle and persisted for 24 h, whereas no change was observed for cytoplasmic and mitochondrial SOD mRNA. Moreover, acute exercise also induced EC-SOD mRNA in the aorta. These results suggest that a single bout of exercise is enough to augment the expression EC-SOD mRNA in skeletal muscle and the aorta, and may partly explain the beneficial effect of exercise. 相似文献
28.
Alteration of Substrate Specificity and Optimum pH of Sarcosine Oxidase by Random and Site-Directed Mutagenesis 下载免费PDF全文
The sarcosine oxidase gene was randomly mutated, and F103V, which showed altered specificity, was screened with the plate assay. Five different mutants at position 103 were also constructed. Each mutant exhibited a rather striking change in substrate specificity, except for F103W. The optimum pH was also shifted, depending on the substrate. 相似文献
29.
Yutaka Nakamura Atsushi Asano Yoshinao Hosaka Takashi Takeuchi Toshihiko Iwanaga Yoshiaki Yamano 《Experimental Animals》2015,64(4):415-424
Membrane trafficking in male germ cells contributes to their development
via cell morphological changes and acrosome formation. TBC family
proteins work as Rab GTPase accelerating proteins (GAPs), which negatively regulate Rab
proteins, to mediate membrane trafficking. In this study, we analyzed the expression of a
Rab GAP, TBC1D9, in mouse organs and the intracellular localization of the gene products.
Tbc1d9 showed abundant expression in adult mice testis. We found that
the Tbc1d9 mRNA was expressed in primary and secondary spermatocytes, and
that the TBC1D9 protein was expressed in spermatocytes and round spermatids. In 293T
cells, TBC1D9-GFP proteins were localized in the endosome and Golgi apparatus.
Compartments that were positive for the constitutive active mutants of Rab7 and Rab9 were
also positive for TBC1D9 isoform 1. In addition, TBC1D9 proteins were associated with Rab7
and Rab9, respectively. These results indicate that TBC1D9 is expressed mainly in
spermatocytes, and suggest that TBC1D9 regulates membrane trafficking pathways related to
Rab9- or Rab7-positive vesicles. 相似文献
30.
Because we found that WTC rats might be resistant to streptozotocin (STZ), we have elucidated the mechanisms of resistant to the diabetogenic effects of STZ in the WTC rats. Dose response to STZ was evaluated with glucose levels. No significant changes in glucose level to STZ administration were observed in WTC rats. Insulin secretion by suppling glucose was preserved in WTC rats even after STZ administration. Although there was no significant difference in gene expression of both GLUT2 and Kir6.2, which were involved in STZ resistance, between WTC rats and Wistar rats, the expression of metallothionein 2a in pancreas and liver to STZ administration of WTC rats was significantly higher than that of Wistar rats. Moreover, alloxan did not induce diabetes in WTC rats as same as STZ. These results suggest that WTC rats might have powerful antioxidant property to protect β cells in pancreas. Because the STZ-resistant property is very close characteristics to human beings, WTC rats will become a useful animal model in diabetic researches. 相似文献