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61.
Shin Masateru; Sakihama Naoko; Koike Hiroyuki; Inoue Yorinao 《Plant & cell physiology》1984,25(8):1575-1578
Two C-type cytochromes, c-550 and c-553, were extracted by animproved procedure from a thermophilic blue-green alga, Synechococcusvulcanus, and effectively purified by a two-step hydrophobicchromatography. The first step was performed with a ToyopearlHW-65C :ammonium sulfate-66 column and the second with a butyl-Toyopearl650 column. This work is the first to apply butyl-toyopearl650 to protein purification. (Received July 2, 1984; Accepted September 13, 1984) 相似文献
62.
Divalent salt-washing of O2-evolving PS II particles caused total liberation of 33-, 24- and 16-kDa proteins, but the resulting PS II particles retained almost all amounts of Mn present in initial particles. The retained Mn was EPR-silent when the particles were kept in high concentrations of divalent salt. By divalent salt-washing, the activity of diphenylcarbazide (DPC) photooxidation was not affected at all, neither suppressed nor enhanced, while O2 evolution was totally inactivated. These results indicate that Mn can be kept associated with PS II particles even after liberation of the 33-kDa protein, and suggest that the 33-kDa protein is probably not responsible for binding Mn onto membranes, but is possibly responsible for maintaining the function of Mn atoms in the O2-evolving center. 相似文献
63.
Treatment of chloroplasts with p-nitrothiophenol in light causesspecific inhibition of photosystem II activity. The inhibitionobeyed first-order kinetics with respect to light intensity.The life time of the light-induced state of chloroplasts, whichis required for p-nitrothiophenol inhibition, was analyzed usingintermittent illumination with flashes at different intervals.The half life time thus determined was 230 msec. (Received August 2, 1976; ) 相似文献
64.
Yorinao Inoue 《BBA》1975,396(3):402-413
The chloroplasts from wheat leaves greened under intermittent illuminations (1 ms in duration) at long intervals (5 min) are capable of photoreducing DCIP (2, 6-dichlorophenolindophenol) with diphenylcarbazide as an electron donor but are incapable of photoreducing DCIP with water as the donor. On exposure of such intermittently illuminated leaves to flashes spaced at intervals of less than 10 s, the delayed light emission from the leaves was greatly enhanced in parallel with the generation of Hill activity. The mechanism of this photoactivation was studied by following the changes of the delayed emission from intermittently illuminated leaves exposed to short-interval flashes programmed in various ways. Analysis of the kinetic data indicated that the photoactivation involves three consecutive photoreactions with a rate-limiting dark reaction between them; P-light → A0-light → A1-dark → A2-light → A3 in which P is a precursor convertible to A0, the first intermediate with a longer lifetime of and A3 is the final activated compound or state converted by short-interval flashes from A0 through A1 and A2, two other intermediates with shorter lifetimes of and 5 s, respectively. 相似文献
65.
The effects of hydrostatic pressure treatments on the photosyntheticactivities of isolated thylakoids and PSII membranes were studied,and the following results were obtained. (1) The O2-evolvingactivity was selectively inhibited by treatment at 200 MPa andabove, while the electron transport in the PSII reaction center,as measured by the photoreduction of 2,6-dichlorophenolindophenol(DCIP) with 1,5-diphenylcarbazide (DPC), was markedly enhanced.(2) The activity of PSI, as measured by the photoreduction ofmethylviologen with reduced DCIP, was not much affected evenafter treatment at 500 MPa, whereas this electron transportbecame uncoupled from phosphorylation at 200 MPa and above.(3) In pressure-treated PSII membranes, the EPR signal of Y+zbecame photoinducible with the concomitant appearance of anEPR signal for Mn2+. (4) The 33-kDa extrinsic protein was retainedin inhibited PSII membranes, but the 17- and 23-kDa extrinsicproteins were lost. (5) Cross-linking of PSII proteins by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide(EDC) suppressed the pressure-induced inactivation of the evolutionof O2. (6) In pressure-treated PSII membranes, higher concentrationsof DCMU were required to inhibit the photoreduction of DCIP.Features of the pressure-induced inhibition of various reactionsin photosynthetic membranes are discussed on the basis of theseresults.
1On leave from Advanced Research Laboratory, Hitachi Ltd., Hatoyama,Saitama, 350-03 Japan. 相似文献