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71.
Peptide sequence analysis using exopeptidases with molecular analysis of the truncated polypeptides by mass spectrometry 总被引:2,自引:0,他引:2
Fast atom bombardment mass spectrometry is used for the analysis of the series of molecular products formed by the cleavage of polypeptide substrates with the exopeptidases carboxypeptidase Y and leucine aminopeptidase. By following the polypeptide molecular species rather than the released residues, sequence information is obtained regardless of the relative rates of cleavage of peptide bonds. In addition, unambiguous assignments of sequence can be made in the presence of multiple identical residues. The lower level of sensitivity for the analysis is in the picomole range. When carboxypeptidase Y is used, the method provides a specific and sensitive method for the sequencing of polypeptides from the C-terminus. 相似文献
72.
73.
<正>Streptomycetes are Gram-positive bacteria with high GC DNA content. They produce the most abundant secondary metabolites including over two-thirds of the clinically used antibiotics of natural origin (Barka et al., 2016), for example,the important broad-spectrum antimicrobials oxytetracycline(OTC) and chlortetracycline, which are the tetracycline antibiotics, produced by Streptomyces rimosus and Strepto- 相似文献
74.
Ma Zhong-You Pu Shun-Chang Jiang Jing-Jing Huang Bo Fan Mei-Zhen Li Zeng-Zhi 《World journal of microbiology & biotechnology》2011,27(3):679-686
A novel gene of thermostable phytase, phyA, was isolated by polymerase chain reaction (PCR) techniques from Aspergillus aculeatus RCEF 4894. The full-length phyA gene comprises 1,404 bp and encodes 467 amino-acid residues, including a 19-residue putative N-terminal signal peptide.
The phytase of A. aculeatus was a novel addition to the histidine-acid phosphatase family, as evidenced by both the conserved motifs RHGXRXP and HD in
the amino-acid sequence, and 3D structure models. The recombinant phytase was overexpressed in Pichia pastoris, and its specific activity reached 3,000 U mL−1 at the optimum pH of 5.5. This recombinant, thermostable phytase was able to withstand temperatures of up to 90 °C for 10 min,
with a loss of only 13.9% of initial enzymatic activity, and showed high activity with phytic-acid sodium salt at a pH range
of 2.5–6.5. The broad pH optima and high thermostability of the phytase makes it a promising candidate for feed-pelleting
applications. 相似文献
75.
Sindermann JR Babij P Klink JC Köbbert C Plenz G Ebbing J Fan L March KL 《American journal of physiology. Heart and circulatory physiology》2002,283(6):H2714-H2724
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy chain promoter. Transgenic mice studied at ages from 3 to 13 wk showed a 3.2-fold increase in arterial wall SMC density, with 28% of SMC exhibiting proliferative cell nuclear antigen staining, confirming enhanced SMC proliferation, which was accompanied by two- to threefold increases in arterial wall areas (P < 0.05). Remarkably, despite increased vessel wall mass, the lumen area was not compromised, but rather was increased. A tightly conserved linear relationship was found between arterial circumference and wall thickness with slopes of 0.036 for both transgenics (r = 0.93, P < 0.01) and controls (r = 0.77, P < 0.01), suggesting the hypothesis that the conservation of wall stress functions as a primary determinant of adaptive arterial remodeling. This establishes a new model of adaptive vessel remodeling occurring in response to a proliferative input in the absence of mechanical injury or primary flow perturbation. 相似文献
76.
目的:观察射频消融术(RFA)联合吉非替尼(Gefitinib)对晚期非小细胞肺癌(NSCLC)治疗的临床效果。方法:26例晚期NSCLC(III、IV期)患者用RFA联合Gefitinib治疗,与23例单独使用RFA治疗的对照组进行比较,观察两组之间有效率、生活质量和不良反应。结果:根据疗效、生活质量评分等指标进行评价,联合治疗组有效率为65.4%,高于单纯RFA治疗组的34.8%,两组之间有显著性差异(P<0.01),对接受治疗的患者进行生活质量评价结果表明,所有接受RFA和Gefitinib联合治疗组中有71.3%的病人生活质量良好,而接受单纯RFA治疗组中只有47%的患者生活质量良好,两组之间有显著性差异(P<0.01),两组患者不良反应主要是疼痛、发热、腹泻等,只需对症处理。结论:RFA联合Gefitinib综合治疗NSCLC方案优于单纯采用RFA的治疗方案。 相似文献
77.
Kim SA Liang CM Cheng IC Cheng YC Chiao MT Tseng CJ Lee F Jong MH Tao MH Yang NS Liang SM 《The journal of gene medicine》2006,8(9):1182-1191
BACKGROUND: Foot-and-mouth disease virus (FMDV) affects susceptible livestock animals and causes disastrous economic impact. Immunization with plasmid expressing VP1 that contains the major antigenic epitope(s) of FMDV as cytoplasmic protein (cVP1) failed to elicit full protection against FMDV challenge. MATERIALS AND METHODS: In this study, mice were immunized via electroporation with four cDNA expression vectors that were constructed to express VP1 of FMDV, as cytoplasmic (cVP1), secreted (sVP1), membrane-anchored (mVP1) or capsid precursor protein (P1), respectively, to evaluate whether expression of VP1 in specific subcellular compartment(s) would result in better immune responses. RESULTS: Electroporation enhanced immune responses to vectors expressing cVP1 or P1 and expedited the immune responses to vectors expressing sVP1 or mVP1. Immunization of mice via electroporation with mVP1 cDNA was better than sVP1 or cVP1 cDNA in eliciting neutralizing antibodies and viral clearance protection. Vaccination with P1 cDNA, nonetheless, yielded the best immune responses and protection among all four cDNAs that we tested. CONCLUSIONS: These results suggest that the antigenicity of a VP1 DNA vaccine can be significantly enhanced by altering the cellular localization of the VP1 antigen. Electroporation is a useful tool for enhancing the immune responses of vectors expressing VP1 or P1. By mimicking FMDV more closely than that of transgenic VP1 and eliciting immune responses favorably toward Th2, transgenic P1 may induce more neutralizing antibodies and better protection against FMDV challenge. 相似文献
78.
79.
Lin Chen Fei He Ruicai Long Fan Zhang Mingna Li Zhen Wang Junmei Kang Qingchuan Yang 《植物学报(英文版)》2021,63(11):1937-1951
Alfalfa (Medicago sativa L.) is an important forage crop worldwide. However, little is known about the effects of breeding status and different geographical populations on alfalfa improvement. Here, we sequenced 220 alfalfa core germplasms and determined that Chinese alfalfa cultivars form an independent group, as evidenced by comparisons of FST values between different subgroups, suggesting that geographical origin plays an important role in group differentiation. By tracing the influence of geographical regions on the genetic diversity of alfalfa varieties in China, we identified 350 common candidate genetic regions and 548 genes under selection. We also defined 165 loci associated with 24 important traits from genome-wide association studies. Of those, 17 genomic regions closely associated with a given phenotype were under selection, with the underlying haplotypes showing significant differences between subgroups of distinct geographical origins. Based on results from expression analysis and association mapping, we propose that 6-phosphogluconolactonase (MsPGL) and a gene encoding a protein with NHL domains (MsNHL) are critical candidate genes for root growth. In conclusion, our results provide valuable information for alfalfa improvement via molecular breeding. 相似文献
80.
牙周病是人类的一种常见病、多发病,其致病菌具有多样性和复杂性,有关该病的致病菌和病因学的研究,对防治工作具有重要意义。作者从牙周病患者的病灶处分离到了一株细菌(90-1),并对其进行了鉴定,现将结果报告如下。 相似文献