Down-regulation of c-Myc expression inhibits the invasion of bile duct carcinoma cells

Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c-Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c-Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down-regulated c-Myc expression can inhibit tumour cell invasion still remains to be explored. The c-Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c-Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c-Myc ASODN could significantly suppress the c-Myc protein expression (P<0.05) and the invasion (P<0.01) of QBC939 cells transfected with c-Myc ASODN compared with that in the control and c-Myc NSODN-transfected group. Thus in the present study we show that down-regulation of c-Myc expression can inhibit the invasion of QBC939 cells in vitro.     

Variation of Intergeneric Somatic Hybrids Between Citrus sinensis cv. Valencia and Fortunella crassifolia cv. Meiwa

Intergeneric somatic hybrid plants derived from protoplast fusion between Citrus sinensis (L.) Osbeck cv. Valencia and Fortunella crassifolia Swingle cv. Meiwa have been grown in the fields for 6 years. The plants grew less vigorously with uneven canopy size and some shoots frequently died. Chromosome count showed that the plants, containing non-tetraploid cells besides amphidiploid, were chimeras. Most somatic hybrid plants exhibited a new band of EST isozyme. RAPD analysis verified that several plants lacked some of the parents' markers. The results indicated that the intergeneric somatic hybrids were genetically unstable.     

近40年来江河源区草地生态压力动态分析

定量化评价人类活动对区域生态系统的压力,对客观认识人文因素干扰区域生态系统的演变程度及效应强度具有重要的意义.在系统总结前人对生态压力指数评价方法的基础上,结合江河源区草地生态系统的现实情况,将生态压力指数定义为草地现实承载力与理论承载力之比,构建了生态压力指数评价模型,并定量分析了江河源区果洛州、海南州、玉树州1961～2002年40a来的生态压力指数.结果表明畜牧业对该区草地生态压力负荷较大;相对于温度和降水等自然因素,以放牧为主的人文因素在短期内对该区草地生态系统演变的作用更加剧烈,从生态压力指数来看,黄河源区下游的海南州的生态压力指数均大于2,已严重超载;同时近40a的生态压力指数的动态变化还揭示了人文因素对区域生态环境演变的影响具有一定的时滞现象,这种时滞现象和人文因素的强度、该区生态系统的承载能力和抗干扰能力具有一定的关系.     

河西走廊中段绿洲退化土地退耕种植苜蓿的固碳效应

土地利用变化和耕作管理是人类影响陆地生态系统碳过程一个重要方面.对河西走廊中段张掖绿洲退化土地退耕种植苜蓿5a后土壤性状的分析表明, 49个退耕苜蓿地土壤与相邻未退耕农田土壤配对样本的比较,退耕苜蓿地0～15cm土层土壤粒级组成和容重并未发生显著变化,但土壤pH平均提高了0.11个单位,电导率降低34.8%,土壤有机碳(SOC)和全氮(全N)含量较对照农田土壤平均提高18.5%和9.3%,活性有机碳(labile C)增加53.3%.SOC含量受海拔高度和土壤粒粉粒含量的影响,退耕后SOC和全N的增加幅度沙壤土高于粉壤土,而labile C的增加幅度沙壤土低于粉壤土.退耕苜蓿地0～15cm土层SOC和全N储量较农田土壤分别增加2.84Mg hm~(-2)和0.21Mg hm~(-2),土壤C、N的固存率平均为0.57Mg hm~(-2)a~(-1)和0.04 Mg hm~(-2)a~(-1),表明退化土地由1年生作物向多年生牧草的转变有显著的固碳效应和潜力.活性有机碳的变化较总有机碳的变化更为显著,表明活性有机碳对土地利用变化的响应更为敏感.     

Mapping Quantitative Trait Loci for Grain Appearance Traits of Rice Using a Recombinant Inbred Line Population

A linkage map consisting of 221 markers was constructed based on a recombinant inbred line (RIL) population from the cross between Zhenshan 97 and Minghui 63. Quantitative trait loci (QTL) mapping was carried out for grain appearance traits such as grain length, grain width and grain shape in rice in 1998 and 1999. Based on interval mapping method at the threshold LOD≥2.4, six, two and two QTLs were detected for grain length, grain width and grain shape, respectively, in 1998; In 1999, three, two and two QTLs were identified for the three traits, respectively. Of them, seven QTLs were simultaneously identified in both of the years. The QTL with large effects located in the interval RG393-C1087 on chromosome 3 not only controlled the grain length, but also influenced the grain shape. It explained 57.5%, 61.4% and 26.7%, 29.9% of phenotypic variation of the grain length and the grain shape in two years, respectively. The QTL with large effects located in the interval RG360-C734B on chromosome 5 affected the grain width and the grain shape. It explained 44.2%, 53.2% and 32.1%, 36.0% of phenotypic variation of the grain width and the shape in two years, respectively. Eight, five and five QTLs were identified for the grain length, width and shape, respectively, based on mixed linear-model composite interval mapping method at P =0.005. Their general contributions were 58.81%, 44.75%, and 57.47%. One QTL for the grain length was found to be significant interaction with environment.     

河南白云山温带落叶阔叶林萌生特征及萌生个体空间分布格局

以河南省白云山自然保护区温带落叶阔叶林5 hm2样地群落为研究对象,分析该样地木本植物的特征,同时利用点分布格局对样地内萌生能力较强的个体进行空间格局分析.结果显示:该样地内共有木本植物91种17369株;发生萌生现象的有62种2082株,萌生物种占总物种数的68.13％,萌生个体占总个体数的11.99％.样地内所有发...     

西藏羊湖地区近1.4万年以来孢粉植物群及古气候研究

通过对西藏羊湖地区ZK1和卡拉苏代牙剖面（IP16）的孢粉分析和总有机碳（TOC）、元素含量分析等,建立了羊湖地区近1.4万年以来的孢粉组合带、重建当时的植被类型及古气候演变序列。结果显示：该时期经历了5次植被类型的更替,即草原型→疏林草原型→草原型→疏林草原型→草原型,与之相对应的气候经历了5次波动（2个半旋回）, 即11 220 a.B.P.左右的第一气候旋回：由干旱→半干旱半湿润转化;8 970a.B.P.左右的第二气候旋回,同样由干旱→半干旱半湿润转化,然后进入半个气候旋回,即干旱气候期,推测今后有可能进入半干旱半湿润气候期。     

克隆整合对无芒雀麦在异质性盐分环境中存活和生长的影响

无芒雀麦是浑善达克沙地植物群落中占优势的多年生根茎禾草.研究了克隆整合特性对无芒雀麦在异质性盐分环境中存活和生长的影响.结果表明,克隆整合显著提高了无芒雀麦分株在高盐环境中的存活能力,耗-益分析表明无芒雀麦在高盐斑块中分株的生物量、分株数、根茎节数和根茎总长显著受益于克隆整合,而与之相连的非盐分斑块中的分株却没有产生显著的损耗.因而,克隆整合特性是无芒雀麦对异质性环境形成的重要适应对策,它使无芒雀麦能够扩展到不适合植物生长的高盐分斑块中,从而增加了无芒雀麦在浑善达克沙地中的存活和生长,提高了其在半干旱沙化地区的适合度.     

河南大别山珍稀濒危植物与保护

研究了河南大别山珍稀濒危植物的种类组成,区系成分,生态地理分布。结果表明本区有珍稀濒危植物18科,22属,24种,其中国家二级保护植物9种,三级保护植物15种,属华南热带植物区系成分的有3种,华东亚热带植物区系成分的有8种,华中亚热带植物区系成分的有10种,全国广布种3种,根据大别山植物的现状提出了本区珍稀濒危保护植物的保护措施,系统清查本区的珍稀濒危植物,建立技术档案,就地建立珍稀濒危植物保育基地;建立以珍稀濒危植物为主体的生态园林示范工程。     

普通野生稻中增产相关QTL的发掘

普通野生稻(Oryza Rufipogon)是重要的遗传资源,发掘其优良等位基因将对水稻遗传改良产生重要影响。文章从以珍汕97为轮回亲本,普通野生稻为供体的BC2F1群体中选择一个与珍汕97表型明显不同的单株BC2F1-15,经过连续自交获得回交重组自交系BC2F5群体。均匀分布于12条染色体的126个多态性SSR(Simplesequence repeats)标记基因型分析,发现BC2F1-15单株在30%的标记位点为杂合基因型;利用该群体共检测到4个抽穗期、3个株高、4个每穗颖花数、2个千粒重和1个单株产量QTL。在第7染色体RM481-RM2区间,检测到抽穗期、每穗颖花数和产量QTL,野生稻等位基因表现增效作用;其他3个每穗颖花数QTL位点,野生稻等位基因也均具有增效作用。结果表明野生稻携带有增产相关的等位基因,这些有利等位基因无疑是水稻遗传改良可资利用的新资源。