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11.
Li-Si Yao Hui Zhao Li-Jun Shao Yong-Xian Liu Xiao-Long Zhang Jing Wang Yong-Qiang Deng Xiao-Feng Li Kong-Xin Hu Cheng-Feng Qin Bao-Liang Xu 《Journal of virology》2012,86(24):13816-13817
Amur virus was recently identified as the causative agent of hemorrhagic fever with renal syndrome. Here we report the complete genome sequence of an Amur virus isolated from Apodemus peninsulae in Northeastern China. The sequence information provided here is critical for the molecular epidemiology and evolution of Amur virus in China. 相似文献
12.
Six phytoecdysterones have been isolated from the n -BuOH portion of Cucubalus baccifer L., a Chinese folk medicinal plant. Their structures were elucidated as ecdysterone (1), 24(28)-ecdysterone (2), 22-deoxyecdysterone (3), 25-hydroxypanuosterone (4), rubrosterone (5) and 2,22-dideoxyecdysterone 3β-O-β- D -glucopyranoside (6) respectively on the basis of spectroscopic and chemical methods. Among them compound 6 was a new phytoecdysterone glycoside and 1-5 were first obtained from this plant. 相似文献
13.
Background
Multiple myeloma (MM) is a disease of cell cycle dysregulation while cell cycle modulation can be a target for MM therapy. In this study we investigated the effects and mechanisms of action of a sesquiterpene lactone 6-O-angeloylplenolin (6-OAP) on MM cells.Methodology/Principal Findings
MM cells were exposed to 6-OAP and cell cycle distribution were analyzed. The role for cyclin B1 to play in 6-OAP-caused mitotic arrest was tested by specific siRNA analyses in U266 cells. MM.1S cells co-incubated with interleukin-6 (IL-6), insulin-like growth factor-I (IGF-I), or bone marrow stromal cells (BMSCs) were treated with 6-OAP. The effects of 6-OAP plus other drugs on MM.1S cells were evaluated. The in vivo therapeutic efficacy and pharmacokinetic features of 6-OAP were tested in nude mice bearing U266 cells and Sprague-Dawley rats, respectively. We found that 6-OAP suppressed the proliferation of dexamethasone-sensitive and dexamethasone-resistant cell lines and primary CD138+ MM cells. 6-OAP caused mitotic arrest, accompanied by activation of spindle assembly checkpoint and blockage of ubiquitiniation and subsequent proteasomal degradation of cyclin B1. Combined use of 6-OAP and bortezomib induced potentiated cytotoxicity with inactivation of ERK1/2 and activation of JNK1/2 and Casp-8/-3. 6-OAP overcame the protective effects of IL-6 and IGF-I on MM cells through inhibition of Jak2/Stat3 and Akt, respectively. 6-OAP inhibited BMSCs-facilitated MM cell expansion and TNF-α-induced NF-κB signal. Moreover, 6-OAP exhibited potent anti-MM activity in nude mice and favorable pharmacokinetics in rats.Conclusions/Significance
These results indicate that 6-OAP is a new cell cycle inhibitor which shows therapeutic potentials for MM. 相似文献14.
Cheng YX Zhou LL Yan YM Chen KX Hou FF 《Bioorganic & medicinal chemistry letters》2011,21(24):7434-7439
Three new cyclic peptides, namely duanbanhuains A–C (1–3), were isolated from the roots of Brachystemma calycinum which is a traditional medicine used to treat rheumatic diseases. Their structures were identified by means of a suite of MS and NMR experiments. These compounds were purposely evaluated for their inhibitory effects on the release of MCP-1, IL-6, collagen IV and reactive oxygen species (ROS) against high-glucose-stimulated mesangial cells. The results showed that compounds 1 and 2 exhibited potent inhibition on the production of IL-6, collagen IV and ROS at the concentration of 10 μM. 相似文献
15.
Zhou XJ Chen XL Li XS Su J He JB Wang YH Li Y Cheng YX 《Bioorganic & medicinal chemistry letters》2011,21(1):373-376
Two new dimeric lignans, zanthpodocarpins A (1) and B (2), and five known lignans, eudesmin (3), (1R,2R,5R,6S)-2-(3,4-dimethoxyphenyl)-6-(3,4-dihydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (4), dimethoxysamin (5), rel-(1R,5R,6S)-6-(4-hydroxy-3-methoxyphenyl)-3,7-dioxabicyclo[3.3.0]octan-2-one (6), and magnone A (7), were isolated from the barks of Zanthoxylum podocarpum. Their structures were identified by using spectroscopic methods. Compounds 1 and 2 are rare dilignans bearing an unusual α,β-unsaturated ketone group from a natural source. Bioassay showed that compounds 1 and 2 could inhibit nitric oxide (NO) production in LPS stimulated RAW 264.7 cells with IC50 values of 5.31 μM and 12.15 μM, respectively. 相似文献
16.
Meyeniines A–C ( 1 – 3 ), three new lignans, two known neolignans ( 4 – 5 ), and three known lignans ( 6 – 8 ) were isolated from the rhizomes of Lepidium meyenii. Their structures were identified by comprehensive spectroscopic analyses and computational methods. Compound 1 represents a unique lignan featuring an aromatic ring migration. Compounds 2 and 4 – 6 were analyzed by chiral HPLC column as enantiomers. Biological evaluation revealed that compound 8 could inhibit IL-6 production in lipopolysaccharide (LPS) induced RAW264.7 cells in a dose-dependent manner. 相似文献
17.
Ibegbu CC Xu YX Harris W Maggio D Miller JD Kourtis AP 《Journal of immunology (Baltimore, Md. : 1950)》2005,174(10):6088-6094
Killer cell lectin-like receptor G1 (KLRG1) is one of several inhibitory killer cell lectin-like receptors expressed by NK cells and T lymphocytes, mainly CD8(+) effector/memory cells that can secrete cytokines but have poor proliferative capacity. Using multiparameter flow cytometry, we studied KLRG1 expression on CD8(+) T cells specific for epitopes of CMV, EBV, influenza, and HIV. Over 92% of CD8(+) cells specific for CMV or EBV expressed KLRG1 during the latent stage of these chronic infections. CD8(+) T cell cells specific for HIV epitopes were mostly (72-89%) KLRG1(+), even though not quite at the level of predominance noted with CMV or EBV. Lower frequency of KLRG1 expression was observed among CD8(+) cells specific for influenza (40-73%), a resolved infection without a latent stage. We further observed that CD8(+) cells expressing CD57, a marker of replicative senescence, also expressed KLRG1; however, a population of CD57(-)KLRG1(+) cells was also identified. This population may represent a "memory" phenotype, because they also expressed CD27, CD28, CCR7, and CD127. In contrast, CD57(+)KLRG1(+) cells did not express CD27, CD28, and CCR7, and expressed CD127 at a much lower frequency, indicating that they represent effector cells that are truly terminally differentiated. The combination of KLRG1 and CD57 expression might thus aid in refining functional characterization of CD8(+) T cell subsets. 相似文献
18.
From the ethanol extract of the roots of Brachystemma calycinum D.Don, a Chinese folk herb, four new minor cyclic peptides namely brachystemin A, B, C and D (1-4) have been isolated. Their structures were established as cyclo (Pro¹-Phe-Leu-Ala¹-Thr-Pro²-Ala²-Gly)(1)、cyclo(Pro¹-Ala-Phe-Trp-Asp-Pro²-Leu-Gly)(2)、cyclo (Pro¹-Ile-Gly-Pro²-Val-Ala¹-Ala²-Tyr) (3) and cyclo (Pro-OMet-Trp-Ile-Gly-Ala-Leu-Asp) (T4) respectively by means of extensive spectral methods. 相似文献
19.
目的:将已成功构建表达anti-CD20scFv/CD80/CD28/zeta转染人T淋巴细胞,体外观察该类细胞特异性清除CD20+原代慢性淋巴细胞白血病(CLL)细胞的能力,为肿瘤的过继免疫治疗提供新思路。方法:将本室成功构建的含anti-CD20scFv/IgGFc/CD80片段的PLNCX质粒,转染PA317包装细胞,挑取高滴度的包装细胞株收获逆转录病毒,用收获的病毒感染刺激分裂的人外周血T细胞,经G418筛选后与CD20+原代CLL细胞在体外共同培养,在显微镜下观察CD20+的原代CLL细胞生长状态,ELISA检测试剂盒检测T细胞分泌细胞因子的功能。结果:重组基因修饰的T细胞能在体外杀伤CD20+原代CLL细胞,而对CD20-细胞无杀伤作用;同时靶细胞为CD20+组上清液中IL-2(1301.00pg/ml)和IFN-γ(602.18pg/ml)水平与CD20-组相比明显升高。结论:嵌合锚定T细胞能够成功构建;该类T细胞在体外能特异性杀伤CD20+的原代CLL细胞。 相似文献
20.
为观察下丘脑胖素 A在哺乳期摄食增加和能量代谢中的作用,本研究采用脑连续切片之免疫组织化学和图像定量分析技术,对分娩后第 12 天非哺乳、持续哺乳、持续哺乳后禁哺乳过夜和持续哺乳 - 禁哺乳后再急性哺乳大鼠下丘脑胖素A免疫反应神经元的免疫反应性进行了观察和半定量分析。结果表明,分娩后持续哺乳 11 d, 大鼠的日摄食量较同期分娩的非哺乳大鼠明显增加(180%),一夜禁哺乳则明显降低哺乳大鼠的日摄食量(45%); 哺乳12 d, 大鼠下丘脑胖素 A免疫反应神经元的数目和平均染色强度较非哺乳大鼠明显增加(P<0.001,P<0.05); 禁哺乳过夜(15 h)明显降低哺乳大鼠胖素A免疫反应神经元的数目和平均染色强度(P<0.001,P<0.05),与非哺乳大鼠比较无明显差异;禁哺乳过夜后再急性哺乳2 h 明显增加禁哺乳大鼠胖素 A 免疫反应神经元的数目和平均染色强度(P<0.001,P<0.05),急性哺乳 5 h 后,虽亦明显增加禁哺乳大鼠胖素 A免疫反应性(P<0.05),但与急性哺乳 2 h 比较作用减弱。上述结果表明,持续哺乳和禁乳后再哺乳均导致下丘脑胖素A明显增加,提示哺乳期胖素A可能表达上调并可能与哺乳期摄食增加有关, 且吸乳动作与下丘脑胖素A样神经元之间可能存在某种神经或体液性联系途径。 相似文献