全文获取类型
收费全文 | 1704篇 |
免费 | 149篇 |
国内免费 | 154篇 |
专业分类
2007篇 |
出版年
2024年 | 4篇 |
2023年 | 26篇 |
2022年 | 73篇 |
2021年 | 88篇 |
2020年 | 62篇 |
2019年 | 89篇 |
2018年 | 84篇 |
2017年 | 43篇 |
2016年 | 75篇 |
2015年 | 131篇 |
2014年 | 141篇 |
2013年 | 129篇 |
2012年 | 161篇 |
2011年 | 141篇 |
2010年 | 86篇 |
2009年 | 78篇 |
2008年 | 90篇 |
2007年 | 69篇 |
2006年 | 74篇 |
2005年 | 50篇 |
2004年 | 38篇 |
2003年 | 38篇 |
2002年 | 43篇 |
2001年 | 23篇 |
2000年 | 29篇 |
1999年 | 16篇 |
1998年 | 11篇 |
1997年 | 17篇 |
1996年 | 12篇 |
1995年 | 19篇 |
1994年 | 7篇 |
1993年 | 10篇 |
1992年 | 6篇 |
1991年 | 9篇 |
1990年 | 10篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 2篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有2007条查询结果,搜索用时 0 毫秒
101.
Yongqing Luo Xueyong Zhao Yuqiang Li Xiaoan Zuo Jie Lian Tao Wang 《Ecological Research》2016,31(4):535-545
Root decomposition is a critical feedback from the plant to the soil, especially in sandy land where strong winds remove aboveground litter. As a pioneer shrub in semi-mobile dunes of the Horqin sandy land, Artemisia halodendron has multiple effects on nutrient capture and the microenvironment. However, its root decomposition has not been studied in terms of its influence on soil organic carbon (SOC) and nitrogen (N). In this study, we buried fine (≤2 mm) and coarse roots in litterbags at a depth of 15 cm below semi-mobile dunes. We measured the masses remaining and the C and N contents at intervals during 434 days of decomposition. The soils below the litterbags were then divided into layers and sampled to measure the SOC and N contents. After rapid initial decomposition, both coarse and fine roots decomposed slowly. After 53 days, 36.2 % of coarse roots and 39.8 % of fine roots had decomposed. In contrast, only 18.4 % of coarse roots and 30.5 % of fine roots decomposed in the following 381 days. Fine roots decomposed significantly faster, and their decomposition rate after the initial rapid decay was strongly related to climate (R 2 = 0.716, P < 0.05). Root decomposition increased SOC and N contents below the litterbags, with larger effects for fine roots. The SOC content was more variable between soil layers than the N content. Thus, decomposition of A. halodendron roots cannot be ignored when studying SOC and N feedbacks from plants to the soil, particularly for fine roots. 相似文献
102.
Jing Li Lugen Zuo Zhijun Geng Qingqing Li Yang Cheng Zi Yang Ruohan Shi Yueqing Zhou Wenhu Nie Yueyue Wang Xiaofeng Zhang Sitang Ge Xue Song Jianguo Hu 《Cell proliferation》2022,55(10)
ObjectivesCrohn''s disease (CD) mesenteric adipose tissue (MAT) inflammation affects enteritis through the interaction between the mesentery and intestine, and we previously found that poorly differentiated mesenteric adipocytes were related to its inflammatory features. Pygopus2 (Pygo2) is a key negative regulator of adipocyte differentiation. We aimed to determine whether Pygo2 participates in CD mesenteric lesions and whether Pygo2 knockdown would be beneficial in a CD model (Il‐10 −/− mice).MethodsPygo2 expression in MAT from control and CD patients and Il‐10 −/− mice was measured by immunohistochemistry. Lentiviral transfection was used to regulate Pygo2 expression in Il‐10 −/− mice, and the effects on mesenteric adipocyte differentiation, inflammation, and dysfunction during spontaneous colitis, as well as the possible mechanism, were investigated.ResultsPygo2 expression was increased in MAT from CD patients and Il‐10 −/− mice, and its expression correlated with poor adipocyte differentiation and inflammation. Pygo2 knockdown significantly ameliorated colitis in Il‐10 −/− mice. Moreover, the downregulation of Pygo2 gene expression could promote adipocyte differentiation and inhibit adipocyte inflammation in vivo and in vitro, and the effects were at least partly mediated by the Axis inhibition protein 2 (Axin2)/glycogen synthase kinase 3 beta (GSK3β) pathway.ConclusionsThe increase in Pygo2 may be related to mesenteric adipocyte poor differentiation and inflammatory features of CD, and Pygo2 inhibition could alleviate CD‐like colitis by improving mesenteric lesions by regulating the Axin2/GSK3β pathway.The increase in Pygopus2 (Pygo2) may be related to mesenteric adipocyte poor differentiation and inflammatory features of CD, and Pygo2 inhibition could alleviate adipocyte differentiation and mesenteric lesions by regulating the Axin2/GSK3β pathway. 相似文献
103.
104.
Jin Wang Kai-Jing Zuo Jie Qin Lida Zhang Lan Su Junrong Liu Hua Ling Jing-Ya Zhao You-Fang Cao Ke-Xuan Tang 《Acta Physiologiae Plantarum》2007,29(1):1-9
A novel cDNA clone encoding a COR413-like gene was isolated by suppression subtraction hybridization and cDNA library screening from sea-island cotton (Gossypium barbadense). This gene (designated as GbCOR413, Accession number: AY761065) has a total length of 893 bp with an open reading frame of 600 bp, encoding a predicated polypeptide
of 200 amino acids with a molecular weight of 22.74 kDa and a predicated pI of 9.2. Bioinformatics analyses revealed that this gene belonged to a novel stress-regulated multi-spanning transmembrane
protein family without signal peptide. By means of semi-quantities RT-PCR analysis, the expression of GbCOR413 under short-term cold treatment at 4°C, water submergence and abscic acid treatment was investigated. Our studies suggested
that the cloned gene was a new member of COR gene family which was slowly responsive to cold stress in cotton.
Jin Wang and Kai-Jing Zuo are co-first authors of this paper. 相似文献
105.
In this paper, a numerically detailed thermodynamic investigation of nucleosomal core particles is presented. The nonlinear Poisson-Boltzmann equation governs the electrostatic properties of both the DNA and histone protein. Brownian dynamics is used as the leading method, in combination with the analysis of the electrical features of the nucleosome. At elevated temperature, the structure of the nucleosome is destabilized by the decrease in electrical interactions of DNA-histone complexes, which can be explained with the EDL theory. Two obvious unwrapping transitions can be found, occurring within the temperature ranges 43-52 and 65-80 degrees C. The first transition is characterized by the melting of DNA terminal domains, and the feature of the second transition is the massive unwrapping of the DNA middle domain. It can be concluded that the nucleosomal DNA consists of two distinct structures, where the DNA terminal domains are less tightly bound to the histone than the DNA middle domain. 相似文献
106.
Zuo Y Zheng H Wang Y Chruszcz M Cymborowski M Skarina T Savchenko A Malhotra A Minor W 《Structure (London, England : 1993)》2007,15(4):417-428
The 3' processing of most bacterial precursor tRNAs involves exonucleolytic trimming to yield a mature CCA end. This step is carried out by RNase T, a member of the large DEDD family of exonucleases. We report the crystal structures of RNase T from Escherichia coli and Pseudomonas aeruginosa, which show that this enzyme adopts an opposing dimeric arrangement, with the catalytic DEDD residues from one monomer closely juxtaposed with a large basic patch on the other monomer. This arrangement suggests that RNase T has to be dimeric for substrate specificity, and agrees very well with prior site-directed mutagenesis studies. The dimeric architecture of RNase T is very similar to the arrangement seen in oligoribonuclease, another bacterial DEDD family exoribonuclease. The catalytic residues in these two enzymes are organized very similarly to the catalytic domain of the third DEDD family exoribonuclease in E. coli, RNase D, which is monomeric. 相似文献
107.
Culture and neural differentiation of rat bone marrow mesenchymal stem cells in vitro 总被引:23,自引:0,他引:23
Lei Z Yongda L Jun M Yingyu S Shaoju Z Xinwen Z Mingxue Z 《Cell biology international》2007,31(9):916-923
Adult bone marrow mesenchymal stem cells (MSCs) can differentiate into several types of mesenchymal cells, including osteocytes, chondrocytes, and adipocytes, but can also differentiate into non-mesenchymal cells, such as neural cells, under appropriate experimental conditions. Until now, many protocols for inducing neuro-differentiation in MSCs in vitro have been reported. But due to the differences in MSCs' isolation and culture conditions, the results of previous studies lacked consistency and comparability. In this study, we induced differentiation into neural phenotype in the same MSCs population by three different treatments: beta-mercaptoethanol, serum-free medium and co-cultivation with fetal mouse brain astrocytes. In all of the three treatments, MSCs could express neural markers such as NeuN or GFAP, associating with remarkable morphological modifications. But these treatments led to neural phenotype in a non-identical manner. In serum-free medium, MSCs mainly differentiated into neuron-like cells, expressing neuronal marker NeuN, and BME can promote this process. Differently, after co-culturing with astrocytes, MSCs leaned to differentiate into GFAP(+) cells. These data confirmed that MSCs can exhibit plastic neuro-differentiational potential in vitro, depending on the protocols of inducement. 相似文献
108.
109.
110.