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51.
Macromolecular affinity labeling 总被引:1,自引:0,他引:1
52.
本文报告了25%烧伤后注射免疫抑制剂(地寨米松磷酸钠)大鼠7种肠道细菌的定量、内脏及血液的细菌培养和肠壁的病理形态学改变。单纯烧伤组(B组)和单纯注射免疫抑制剂组(Ⅰ组)动物肠壁的病理改变较轻,肠道菌群改变不明显,其肠道细菌易位率也很低。烧伤后注射免疫抑制剂的动物(BⅠ组)肠壁病变严重,肠道菌群改变明显。其中,肠杆菌在回肠、盲肠,结肠内容物中的菌量均明显升高;回肠内容物中拟杆菌的菌量明显减少;该组动物肠道细菌易位率也明显增高,达60%。此外,BⅠ组、Ⅰ组部分动物发生了由棒状杆菌及由棒状扦菌和肠杆菌混合引起的内源性感染。根据不同肠段肠壁病变严重程度、菌群变化的部位以及不同部位肠管生理功能的不同,我们推测,回肠以上肠段是肠道细菌易位的主要部位。 相似文献
53.
Surjit Singh Gernot Fritze Bingliang Fang Shoji Harada Yong K. Paik Rolf Eckey Dharam P. Agarwal H. Werner Goedde 《Human genetics》1989,83(2):119-121
Summary Genotyping of mitochondrial aldehyde dehydrogenase (ALDH I) was performed in enzymatically amplified DNA of 20 Chinese, Japanese and South Korean families (85 individuals) and in 113 unrelated persons by employing allele-specific oligonucleotide probes and dot blot hybridization. Genotyping individuals with phenotypic deficiency of ALDH I activity always showed the presence of at least one mutant allele. The data are compatible with a model assuming dominant inheritance of the mutant allele, which we have previously suggested on the basis of a population study. 相似文献
54.
The conversion of inter-alpha-trypsin inhibitor (I alpha I) into active, acid-stable derivatives by proteolytic degradation has been tested with 10 different proteinases. Of these, only plasma kallikrein, cathepsin G, neutrophil elastase, and the Staphylococcus aureus V-8 proteinase were found to be effective, each releasing more than 50% of this activity. However, a strong correlation between inhibitor degradation and significant release of acid-stable activity could only be found with the V-8 enzyme. Inhibition kinetics for the interaction of native I alpha I, the inhibitory fragment released by digestion with S. aureus V-8 proteinase, or the related urinary trypsin inhibitor, with seven different proteinases indicated that all had essentially identical Ki values with an individual enzyme and, where measurements were possible, nearly identical second order association rate constants. Significantly, none of the five human proteinases tested, including trypsin, chymotrypsin, plasmin, neutrophil elastase, and cathepsin G, would appear to have low enough Ki values to be physiologically relevant. Thus, the role of native I alpha I or its degradation products in controlling a specific proteolytic activity is still unknown. 相似文献
55.
Reduced biopterin as a cofactor in the generation of nitrogen oxides by murine macrophages 总被引:34,自引:0,他引:34
Generation of nitric oxide (NO.), an autacoid with vasorelaxant and cytotoxic properties, requires at least three cytosolic components in mouse macrophages besides L-arginine and NADPH. One or more components appear after induction by immunologic stimuli; two or more are present in both activated and non-activated macrophages. The constitutive factors can be separated on a Mr approximately 30,000 cut-off filter into high Mr fraction (HF) and low Mr fraction (LF) (Stuehr, D. J., Kwon, N. S., Gross, S. S., Thiel, B. A., Levi, R., and Nathan, C. F. (1989) Biochem. Biophys. Res. Commun. 161, 420-426). Herein we characterize the major active component in LF. The active component was dialyzable (Mr less than approximately 1,000), water soluble, and cationic at acidic to neutral pH. Fractionation on a C18 column in an acetonitrile/water gradient yielded one broad peak of activity, most of which corresponded to a fluorophore with the excitation/emission spectra of biopterins. Gas chromatography isolated a species in this peak with the mass spectrum of biopterin. Of 14 pteridines tested, only 7,8-dihydrobiopterin (H2biopterin) or 5,6,7,8-tetrahydrobiopterin (H4biopterin) could replace LF in synergizing with HF and the inducible component(s) to generate NO-2 and NO-3, the accumulating oxidation products of NO.. Half-maximal activity required 20-30 nM reduced biopterins. LFs from three cell lines were active in proportion to their content of biopterins; addition of reduced biopterins restored activity to LF from biopterin-deficient cells. Enhancement of NO-2 generation in the presence of H2biopterin but not H4biopterin was abolished by methotrexate and aminopterin, inhibitors of dihydrofolate reductase. These findings implicate a redox cycle in which the generation of NO. is facilitated by catalytic amounts of H4biopterin. 相似文献
56.
Summary Fifteen strains of yeast, which produced an extracellular amylolytic enzymes, were isolated from nature. One of them produced more than 100 times the enzyme activity in comparison with the 14 strains and the extremely hyperproducing strain of yeast was identified asCandida
sp. 347. Paper chromatograms of the amylolytic enzyme demonstrated activity of amyloglucosidase. The optimum pH for activity of the enzyme was 5.5–6.0 and optimum temperature was 60°C. 相似文献
57.
Determination of the entire nucleotide sequence of the aphid 28S ribosomal RNA gene (28S rDNA) revealed that it is 4,147 by
in length with a G + C content of 60.3%. Based on the nucleotide sequence, we constructed a presumed secondary-structure model
of the aphid 28S rRNA which indicated that the aphid 28S rRNA is characterized by the length and high G + C content of its
variable regions. The G + C content of the aphid's variable regions was much higher than that of the entire sequence of the
28S rRNA, which formed a striking contrast to those ofDrosophila with the G + C content much lower than the entire 28S molecule. In this respect, the aphid 28S rRNA somewhat resembled those
of vertebrates. This is the third report of a complete large-subunit rRNA sequence from an arthropod, and the first 28S rRNA
sequence for a nondipterous insect.
Correspondence to: H. Ishikawa 相似文献
58.
Chronic In Vivo Sodium Azide Infusion Induces Selective and Stable Inhibition of Cytochrome c Oxidase 总被引:2,自引:1,他引:1
M. Catherine Bennett Gary W. Mlady Young-Hwa Kwon †‡ Gregory M. Rose 《Journal of neurochemistry》1996,66(6):2606-2611
Abstract: The effect of chronic subcutaneous infusion of sodium azide on the activity of mitochondrial respiratory chain enzymes was investigated in Sprague-Dawley rats. Treatment with ∼1 mg/kg/h sodium azide induced chronic, partial inhibition of cytochrome c oxidase, whereas the activities of respiratory complexes I and III were not significantly affected. The inhibition of cytochrome c oxidase was evident by 7 days after infusion began, and the effect was stable for at least 3 weeks. The selectivity of azide for cytochrome c oxidase is discussed in the context of other findings of azide effects on enzymes. The results of the present study indicate that the sodium azide infusion paradigm described here provides a useful tool for the evaluation of selective and stable cytochrome oxidase inhibition in vivo. 相似文献
59.
Transdominant mutants of I kappa B alpha block Tat-tumor necrosis factor synergistic activation of human immunodeficiency virus type 1 gene expression and virus multiplication. 总被引:2,自引:1,他引:1 下载免费PDF全文
P Beauparlant H Kwon M Clarke R Lin N Sonenberg M Wainberg J Hiscott 《Journal of virology》1996,70(9):5777-5785
60.
神经敏感性的降低是棉铃虫对拟除虫菊酯抗药性的重要机制 总被引:11,自引:3,他引:8
用扫描电子显微镜和光学显微镜的染色观察,研究了棉铃虫Helicoverpa armigeraHubner 3龄幼虫腹部神经一肌肉的分布,表明其腹纵肌细胞适合于作电生理细胞内记录。用电生理细胞内微电极记录法研究了棉铃虫三个品系:对照品系、功夫菊酯抗性品系(Cy-R,抗性指数13.4)和氰戊菊酯抗性品系(Fn-R,抗性指数37.9)的3龄幼虫神经一肌肉材料对相应杀虫剂的神经敏感性。结果表明:用10-6mol/L的功夫菊酯处理Cy-R,在30min内有40%的个体对药剂无反应,另外60%的个体产生反应所需的时间是对照品系的3.5倍。用10-6mol/L的氰戊菊酯处理Fn-R,在30min内有47%的个体对药剂无反应,另外53%的个体产生反应所需的时间是对照品系的4.2倍。这些结果说明神经敏感性降低是棉铃虫对拟除虫菊酯抗药性的重要机制。 相似文献