全文获取类型
收费全文 | 9802篇 |
免费 | 656篇 |
国内免费 | 1篇 |
专业分类
10459篇 |
出版年
2022年 | 52篇 |
2021年 | 93篇 |
2020年 | 54篇 |
2019年 | 78篇 |
2018年 | 109篇 |
2017年 | 91篇 |
2016年 | 129篇 |
2015年 | 213篇 |
2014年 | 288篇 |
2013年 | 665篇 |
2012年 | 440篇 |
2011年 | 447篇 |
2010年 | 295篇 |
2009年 | 289篇 |
2008年 | 449篇 |
2007年 | 505篇 |
2006年 | 449篇 |
2005年 | 447篇 |
2004年 | 476篇 |
2003年 | 418篇 |
2002年 | 475篇 |
2001年 | 301篇 |
2000年 | 340篇 |
1999年 | 304篇 |
1998年 | 126篇 |
1997年 | 106篇 |
1996年 | 72篇 |
1995年 | 108篇 |
1994年 | 82篇 |
1993年 | 96篇 |
1992年 | 186篇 |
1991年 | 237篇 |
1990年 | 184篇 |
1989年 | 175篇 |
1988年 | 199篇 |
1987年 | 171篇 |
1986年 | 148篇 |
1985年 | 142篇 |
1984年 | 114篇 |
1983年 | 89篇 |
1982年 | 73篇 |
1981年 | 74篇 |
1980年 | 61篇 |
1979年 | 75篇 |
1978年 | 73篇 |
1977年 | 48篇 |
1976年 | 45篇 |
1975年 | 48篇 |
1974年 | 45篇 |
1973年 | 48篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Yoko Marutani Yasuo Yamauchi Mari Higashiyama Akihito Miyoshi Seiji Akimoto Kanako Inoue Ken-ichi Ikeda Masaharu Mizutani Yukihiro Sugimoto 《Photosynthesis research》2017,131(1):41-50
Light and temperature affect state transitions through changes in the plastoquinone (PQ) redox state in photosynthetic organisms. We demonstrated that light and/or heat treatment induced preferential photosystem (PS) I excitation by binding light-harvesting complex II (LHCII) proteins. The photosystem of wheat was in state 1 after dark overnight treatment, wherein PQ was oxidized and most of LHCII was not bound to PSI. At the onset of the light treatment [25 °C in the light (100 µmol photons m?2 s?1)], two major LHCIIs, Lhcb1 and Lhcb2 were phosphorylated, and the PSI–LHCII supercomplex formed within 5 min, which coincided with an increase in the PQ oxidation rate. Heat treatment at 40 °C of light-adapted wheat led to further LHCII protein phosphorylation of, resultant cyclic electron flow promotion, which was accompanied by ultrafast excitation of PSI and structural changes of thylakoid membranes, thereby protecting PSII from heat damage. These results suggest that LHCIIs are required for the functionality of wheat plant PSI, as it keeps PQ oxidized by regulating photochemical electron flow, thereby helping acclimation to environmental changes. 相似文献
102.
Chiba Y Oshima K Arai H Ishii M Igarashi Y 《The Journal of biological chemistry》2012,287(15):11934-11941
Phosphoserine phosphatase (PSP) catalyzes the dephosphorylation of phosphoserine to serine and inorganic phosphate. PSPs, which have been found in all three domains of life, belong to the haloacid dehalogenase-like hydrolase superfamily. However, certain organisms, particularly bacteria, lack a classical PSP gene, although they appear to possess a functional phosphoserine synthetic pathway. The apparent lack of a PSP ortholog in Hydrogenobacter thermophilus, an obligately chemolithoautotrophic and thermophilic bacterium, represented a missing link in serine anabolism because our previous study suggested that serine should be synthesized from phosphoserine. Here, we detected PSP activity in cell-free extracts of H. thermophilus and purified two proteins with PSP activity. Surprisingly, these proteins belonged to the histidine phosphatase superfamily and had been annotated as cofactor-dependent phosphoglycerate mutase (dPGM). However, because they possessed neither mutase activity nor the residues important for the activity, we defined these proteins as novel-type PSPs. Considering the strict substrate specificity toward l-phosphoserine, kinetic parameters, and PSP activity levels in cell-free extracts, these proteins were strongly suggested to function as PSPs in vivo. We also detected PSP activity from "dPGM-like" proteins of Thermus thermophilus and Arabidopsis thaliana, suggesting that PSP activity catalyzed by dPGM-like proteins may be distributed among a broad range of organisms. In fact, a number of bacterial genera, including Firmicutes and Cyanobacteria, were proposed to be strong candidates for possessing this novel type of PSP. These findings will help to identify the missing link in serine anabolism. 相似文献
103.
104.
Kouji Iguchi Katsuhito Kawato Tetsurou Seita Takashi Kuribayashi Tokihiro Shimada Mariko Matsumoto Yutaka Yamamoto Manabu Yamada Shizuo Yamamoto 《Experimental Animals》2007,56(1):43-49
This study was undertaken to investigate whether the concentration of carbonic anhydorase isoenzyme I (CA-I) in canine feces and urine is useful as a temporary marker of occult blood. Concentrations of CA-I were measured by enzyme-linked immunosorbent assay (ELISA). Fecal CA-I concentrations in 113 healthy beagle dogs (50 male and 63 female) of various ages ranged from 4.3 to 16.7 ng/g feces (mean; 7.0 +/- 2.9 ng/g feces). One milliliter of blood from 3 healthy beagle dogs was found to contain 1,047, 1,062 and 1,150 microg CA-I. The fecal CA-I concentrations of dogs receiving intragastric infusions of autologous blood (10 ml) were very low. However, the fecal CA-I concentrations of dogs receiving infusion of autologous blood (5 ml) into the ascending colon were very high. Detection of fecal CA-I would be useful for identifying dogs with hemorrhaging of the large intestine. Of 55 urinary samples collected from healthy beagle dogs by catheter, chemical tests for occult blood were negative in 44, but CA-I concentrations ranged from 1.8 to 12.6 ng/ml (mean; 6.9 +/- 5.4 ng/ml) by ELISA. The CA-I concentrations of the other 11 samples, which tested positive for occult blood on chemical testing, ranged from 41.2 to 525.0 ng/ml by ELISA. Although CA-I is not a specific marker of erythrocytes, CA-I may be used to detect occult blood in canine feces and urine until a specific immunological test kit using antibody for Hb is developed. 相似文献
105.
Tokuhisa Abo Ryo‐hei Iida Syuhei Kaneko Takeo Suga Hiroyuki Yamada Yoshiki Hamada Akira Yamane 《Cell biochemistry and function》2012,30(8):671-676
Clenbuterol, a β2‐adrenergic agonist, increases the hypertrophy of skeletal muscle. Insulin‐like growth factor (IGF) is reported to work as a potent positive regulator in the clenbuterol‐induced hypertrophy of skeletal muscles. However, the precise regulatory mechanism for the hypertrophy of skeletal muscle induced by clenbuterol is unknown. Myostatin, a member of the TGFβ super family, is a negative regulator of muscle growth. The aim of the present study is to elucidate the function of myostatin and IGF in the hypertrophy of rat masseter muscle induced by clenbuterol. To investigate the function of myostatin and IGF in regulatory mechanism for the clenbuterol‐induced hypertrophy of skeletal muscles, we analysed the expression of myostatin and phosphorylation levels of myostatin and IGF signaling components in the masseter muscle of rat to which clenbuterol was orally administered for 21 days. Hypertrophy of the rat masseter muscle was induced between 3 and 14 days of oral administration of clenbuterol and was terminated at 21 days. The expression of myostatin and the phosphorylation of smad2/3 were elevated at 21 days. The phosphorylation of IGF receptor 1 (IGFR1) and akt1 was elevated at 3 and 7 days. These results suggest that myostatin functions as a negative regulator in the later stages in the hypertrophy of rat masseter muscle induced by clenbuterol, whereas IGF works as a positive regulator in the earlier stages. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
106.
107.
Jens M. Olesen Yoko L. Dupont Bodil K. Ehlers Alfredo Valido Dennis M. Hansen 《Nordic Journal of Botany》2003,23(5):537-539
Jasminum odoratissimum is a Madeira and Canary Islands endemic showing classic heterostyly, i.e. with long-styled flowers with anthers at a low level in the corolla tube and short-styled flowers with anthers at a high level in the corolla tube. Short-styled flowers have large pollen, whereas long-styled flowers have small pollen. The two types are present in equal frequencies in the population. 相似文献
108.
Summary We studied the histochemical distribution of zinc in rat epididymis using a sulphide-silver method. In the supranuclear cytoplasm of the principal cells that line the epididymis of rats, varying amounts of sulphide-silver-reactive zinc were visualized. In adult mating rats, significant amounts of zinc were found in the proximal portion of the epididymis, whereas in non-mating, mature and immature young rats, this heavy metal was most prominent in the distal portion of this organ. In all of the rats studied, zinc was sparsely distributed in the intermediate portion of the epididymis. From these results, it can be assumed that the zinc present in the epithelial lining of rat epididymis plays an important role in the maturation of spermatozoa. The present results represent a useful contribution to our understanding of the functional morphology of rat epididymis.Dedicated to Professor Dr. T.H.Schiebler on the occasion of his 65th birthday 相似文献
109.
Mulla Aziz J. Lin Che-Hung Takahashi Shunichi Nozawa Yoko 《Coral reefs (Online)》2021,40(4):1297-1306
Coral Reefs - Behaviour can have profound consequences for the dispersal potential of an organism. In the marine environment, larvae rely heavily on oceanic currents to migrate from one area to... 相似文献
110.
A novel enzyme, arylalkyl acylamidase, which shows a strict specificity for N-acetyl arylalkylamines, but not acetanilide derivatives, was purified from the culture broth of Pseudomonas putida Sc2. The purified enzyme appeared to be homogeneous, as judged by native and SDS/PAGE. The enzyme has a molecular mass of approximately 150 kDa and consists of four identical subunits. The purified enzyme catalyzed the hydrolysis of N-acetyl-2-phenylethylamine to 2-phenylethylamine and acetic acid at the rate of 6.25 mumol.min-1.mg-1 at 30 degrees C. It also catalyzed the hydrolysis of various N-acetyl arylalkylamines containing a benzene or indole ring, and acetic acid arylalkyl esters. The enzyme did not hydrolyze acetanilide, N-acetyl aliphatic amines, N-acetyl amino acids, N-acetyl amino sugars or acylthiocholine. The apparent Km for N-acetylbenzylamine, N-acetyl-2-phenylethylamine and N-acetyl-3-phenylpropylamine are 41 mM, 0.31 mM and 1.6 mM, respectively. The purified enzyme was sensitive to thiol reagents such as Ag2SO4, HgCl2 and p-chloromercuribenzoic acid, and its activity was enhanced by divalent metal ions such as Zn2+, Mg2+ and Mn2+. 相似文献