全文获取类型
收费全文 | 468篇 |
免费 | 24篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 7篇 |
2020年 | 4篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2016年 | 8篇 |
2015年 | 18篇 |
2014年 | 20篇 |
2013年 | 36篇 |
2012年 | 26篇 |
2011年 | 26篇 |
2010年 | 20篇 |
2009年 | 16篇 |
2008年 | 24篇 |
2007年 | 34篇 |
2006年 | 17篇 |
2005年 | 35篇 |
2004年 | 34篇 |
2003年 | 29篇 |
2002年 | 36篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 12篇 |
1997年 | 8篇 |
1996年 | 1篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1993年 | 7篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有492条查询结果,搜索用时 31 毫秒
41.
42.
43.
44.
Soichiro Tabuchi Junji Ito Takashi Adachi Hiroki Ishida Yoji Hata Fumiyoshi Okazaki Tsutomu Tanaka Chiaki Ogino Akihiko Kondo 《Applied microbiology and biotechnology》2010,87(5):1783-1789
A novel cell surface display system in Aspergillus oryzae was established by using a chitin-binding module (CBM) from Saccharomyces cerevisiae as an anchor protein. CBM was fused to the N or C terminus of green fluorescent protein (GFP) and the fusion proteins (GFP-CBM
and CBM-GFP) were expressed using A. oryzae as a host. Western blotting and fluorescence microscopy analysis showed that both GFP-CBM and CBM-GFP were successfully expressed
on the cell surface. In addition, cell surface display of triacylglycerol lipase from A. oryzae (tglA), while retaining its activity, was also successfully demonstrated using CBM as an anchor protein. The activity of
tglA was significantly higher when tglA was fused to the C terminus than N terminus of CBM. Together, these results show that
CBM used as a first anchor protein enables the fusion of both the N and/or C terminus of a target protein. 相似文献
45.
Tetsu Yamane Masako Mitsumata Noriko Yamaguchi Tadao Nakazawa Kunio Mochizuki Tetsuo Kondo Tomonori Kawasaki Shin-ichi Murata Yoji Yoshida Ryohei Katoh 《Cell and tissue research》2010,340(3):471-479
Remodeling of endothelial basement membrane is important in atherogenesis. Since little is known about the actual relationship
between type IV collagen and matrix metalloprotease−2 (MMP-2) in endothelial cells (ECs) under shear stress by blood flow,
we performed quantitative analysis for type IV collagen and MMP-2 in ECs under high shear stress. The mRNA of type IV collagen
from ECs exposed to high shear stress (10 and 30 dyn/cm2) had a higher expression compared to ECs exposed to a static condition or low shear stress (3 dyn/cm2) (P < 0.01). 3H-proline uptake analysis and fluorography revealed a remarkable increase of type IV collagen under high shear stress (P < 0.01). In contrast, zymography revealed that exposing to high shear stress, however similar positivity was leveled in the
intracellular MMP-2 in the control and high shear stress-exposed ECs, reduced the secretion of MMP-2 in ECs. The results of
Northern blotting, gelatin zymography and monitoring the intracellular trafficking of GFP-labeled MMP-2 revealed that MMP-2
secretion by ECs was completely suppressed by high shear stress, but the intracellular mRNA expression, protein synthesis,
and transport of MMP-2 were not affected. In conclusion, we suggest that high shear stress up-regulates type IV collagen synthesis
and down-regulates MMP-2 secretion in ECs, which plays an important role in remodeling of the endothelial basement membrane
and may suppress atherogenesis. 相似文献
46.
Takashi Matozaki Yoji Murata Munemasa Mori Takenori Kotani Hideki Okazawa Hiroshi Ohnishi 《Cellular signalling》2010,22(12):1811-1817
The R3 subtype of receptor-type protein tyrosine phosphatases (RPTPs) includes VE-PTP, DEP-1, PTPRO, and SAP-1. All of these enzymes share a similar structure, with a single catalytic domain and putative tyrosine phosphorylation sites in the cytoplasmic region and fibronectin type III–like domains in the extracellular region. The expression of each R3 RPTP is largely restricted to a single or limited number of cell types, with VE-PTP and DEP-1 being expressed in endothelial or hematopoietic cells, PTPRO in neurons and in podocytes of the renal glomerulus, and SAP-1 in gastrointestinal epithelial cells. In addition, these RPTPs are localized specifically at the apical surface of polarized cells. The structure, expression, and localization of the R3 RPTPs suggest that they perform tissue-specific functions and that they might act through a common mechanism that includes activation of Src family kinases. In this review, we describe recent insights into R3-subtype RPTPs, particularly those of mammals. 相似文献
47.
Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival 总被引:1,自引:0,他引:1
Mueller KL Thomas MS Burbach BJ Peterson EJ Shimizu Y 《Journal of immunology (Baltimore, Md. : 1950)》2007,179(6):3559-3569
The hemopoietic specific adapter protein ADAP (adhesion and degranulation-promoting adapter protein) positively regulates TCR-dependent, integrin-mediated adhesion and participates in signaling pathways downstream of the TCR that result in T cell activation. The specific role of ADAP in regulating Ag-dependent T cell interactions with APCs and T cell activation following Ag stimulation is not known. We used ADAP-/- DO11.10 T cells to demonstrate that ADAP promotes T cell conjugation to Ag-laden APCs. Complementary in vitro and in vivo approaches reveal that ADAP controls optimal T cell proliferation, cytokine production, and expression of the prosurvival protein Bcl-xL in response to limiting Ag doses. Furthermore, ADAP is critical for clonal expansion in vivo independent of Ag concentration under conditions of low clonal abundance. These results suggest that ADAP regulates T cell activation by promoting Ag-dependent T cell-APC interactions, resulting in enhanced T cell sensitivity to Ag, and by participating in prosurvival signaling pathways initiated by Ag stimulation. 相似文献
48.
Jin D Ueda H Takai S Muramatsu M Furubayashi K Ibaraki T Kishi K Katsuoka Y Miyazaki M 《Life sciences》2007,81(16):1291-1300
Chymase is an important enzyme for the generation of angiotensin (Ang) II and in the activation of transforming growth factor (TGF)-beta1. Therefore, chymase may be involved in the hemodialysis access dysfunction, which is caused by intimal hyperplasia that occurs after polytetrafluoroethylene (PTFE) graft implantations. Bilateral U-shaped PTFE grafts were placed between the femoral vein and artery in dogs. Chymase inhibitor (NK3201, 1 mg/kg per day, p.o.) treatments were initiated 3 days before the operation. After the implantation, the stenosis by neointima proliferation was most frequently observed in the venous side of the PTFE grafts. In the hyperplastic neointima, myofibroblasts were the main cellular components. On the other hand, fibroblasts only occupied cellular components in a much smaller proportion in the neointima. However, these cells seem to be rich in the properties of proliferation and migration. After PTFE graft implantations, extensive accumulations of chymase-positive mast cells were found mainly in the tissue surrounding the grafts. The Ang II- and TGF-beta-positive cells were found in an adjacent section that was in close proximity to the chymase-positive cells. In contrast, the AT(1) receptors, as well as TGF-beta type II receptors, were expressed either in the neointima or in the outside adventitia of the PTFE grafts. Chymase inhibitor treatment resulted in a reduction of chymase, Ang II and TGF-beta1 expression, leading to a significant inhibition of neointimal formation. These findings indicating that an increase of chymase via promoting Ang II and TGF-beta1 generation plays a pivotal role in the neointimal formation after the implantation of PTFE grafts and also suggesting that chymase inhibition may be a new strategy that can be used to prevent PTFE graft dysfunctions in clinical settings. 相似文献
49.
50.
Takahashi R Kuramochi T Aoyagi K Hashimoto S Miyoshi I Kasai N Hakamata Y Kobayashi E Ueda M 《Transgenic research》2007,16(1):115-120
Cell marking is a very important procedure for identifying donor cells after cell and/or organ transplantation in vivo. Transgenic
animals expressing marker proteins such as enhanced green fluorescent protein (EGFP) in their tissues are a powerful tool
for research in fields of tissue engineering and regenerative medicine. The purpose of this study was to establish transgenic
rabbit lines that ubiquitously express EGFP under the control of the cytomegalovirus immediate early enhancer/beta-actin promoter
(CAG) to provide a fluorescent transgenic animal as a bioresource. We microinjected the EGFP expression vector into 945 rabbit
eggs and 4 independent transgenic candidate pups were obtained. Two of them died before sexual maturation and one was infertile.
One transgenic male candidate founder rabbit was obtained and could be bred by artificial insemination. The rabbit transmitted
the transgene in a Mendelian manner. Using fluorescence in situ hybridization analysis, we detected the transgene at 7q11
on chromosome 7 as a large centromeric region in two F1 offspring (one female and one male). Eventually, one transgenic line
was established. Ubiquitous EGFP florescence was confirmed in all examined organs. There were no gender-related differences
in fluorescence. The established CAG/EGFP transgenic rabbit will be an important bioresource and a useful tool for various
studies in tissue engineering and regenerative medicine. 相似文献