全文获取类型
收费全文 | 1623篇 |
免费 | 102篇 |
出版年
2024年 | 2篇 |
2023年 | 5篇 |
2022年 | 13篇 |
2021年 | 15篇 |
2020年 | 14篇 |
2019年 | 16篇 |
2018年 | 27篇 |
2017年 | 24篇 |
2016年 | 43篇 |
2015年 | 54篇 |
2014年 | 64篇 |
2013年 | 127篇 |
2012年 | 99篇 |
2011年 | 98篇 |
2010年 | 63篇 |
2009年 | 57篇 |
2008年 | 101篇 |
2007年 | 97篇 |
2006年 | 106篇 |
2005年 | 111篇 |
2004年 | 101篇 |
2003年 | 107篇 |
2002年 | 100篇 |
2001年 | 11篇 |
2000年 | 11篇 |
1999年 | 21篇 |
1998年 | 20篇 |
1997年 | 25篇 |
1996年 | 19篇 |
1995年 | 20篇 |
1994年 | 20篇 |
1993年 | 15篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 11篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1982年 | 4篇 |
1981年 | 13篇 |
1980年 | 3篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1973年 | 4篇 |
排序方式: 共有1725条查询结果,搜索用时 15 毫秒
91.
Qiu YL Sekiguchi Y Imachi H Kamagata Y Tseng IC Cheng SS Ohashi A Harada H 《Archives of microbiology》2003,179(4):242-249
An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FB(T), was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 degrees C, and the optimum pH was 7.0-7.2. A co-culture with the hydrogenotrophic methanogen Methanospirillum hungatei converted benzoate to acetate, carbon dioxide, and methane. Butyrate transiently accumulated at a high concentration of 2.5 mM during degradation. Besides benzoate, no other compound tested supported growth of the co-culture. Crotonate supported growth of strain FB(T) in pure culture. Furthermore, the strain degraded benzoate in pure culture with crotonate as co-substrate to produce acetate and butyrate. The strain was not able to utilize sulfate, sulfite, thiosulfate, nitrate, fumarate, or Fe(III) as electron acceptor. The G+C content of the DNA was 46.8 mol%. Strain FB(T) contained MK-7 as the major quinone and C(16:1) as the major fatty acid. 16S rDNA sequence analysis revealed that the strain was a member of the genus Sporotomaculum, even though it exhibited significant differences, such as the capacity for syntrophic growth, to the known member of the genus. Hence, we propose the name Sporotomaculum syntrophicum sp. nov. for strain FB(T). The type strain is strain FB(T) (DSM 14795, JCM 11475). 相似文献
92.
93.
94.
Insect cytokine growth-blocking peptide triggers a termination system of cellular immunity by inducing its binding protein 总被引:2,自引:0,他引:2
Growth-blocking peptide (GBP) is a 25-amino acid cytokine found in lepidopteran insects that possesses diverse biological activities such as stimulation of immune cells (plasmatocytes), cell proliferation, and larval growth regulation. We found another novel function of GBP that induces a hemolysis of another class of blood cells (oenocytoids). In the lysate of oenocytoids we identified a GBP-binding protein that shows a specific affinity for GBP. The characterization of purified GBP-binding protein and its cDNA demonstrated it as a 49.5-kDa novel protein with a C-terminal region displaying limited homology to several insect lipoproteins. Results of Northern and Western blotting indicated that the GBP-binding protein should be synthesized only in blood cells. Immunoelectron microscopic analyses confirmed that indirect immunoreactive signals were mostly localized in oenocytoids. Kinetic and biological analyses of interaction between GBP and the binding protein showed their strong binding was followed by clearance of GBP from hemolymph, thus indicating that this protein might function as an inhibitory factor against GBP. Based on these results, we propose that insect cytokine GBP shows multifunctions even in cellular immunity: it serves to stimulate immune cells and afterward silences its own action by inducing the binding protein through specific hemolysis. 相似文献
95.
96.
97.
Amirta R Fujimori K Shirai N Honda Y Watanabe T 《Chemistry and physics of lipids》2003,126(2):121-131
A lignin-degrading basidiomycete, Ceriporiopsis subvermispora produces a series of alkyl- and alkenylitaconates (ceriporic acids). Previously, two alkylitaconic acids with tetradecyl and hexadecyl side chains were isolated and identified as 1-heptadecene-2,3-dicarboxylic acid (ceriporic acid A) and 1-nonadecene-2,3-dicarboxylic acid (ceriporic acid B). In the present study, one hexadecenylitaconate (ceriporic acid C) was isolated and its chemical structure was analyzed by glycolation and subsequent (1) trimethylsilation, or (2) acetalation with acetone and acetone-d6. Analyses of the isolated metabolite demonstrated that the hexadecenylitaconic acid was (Z)-1,10-nonadecadiene-2,3-dicarboxylic acid. The structure of the side chain in ceriporic acid C was the same as that of hexadecenylcitraconate, chaetomellic acid B. Thus, it was found that ceriporic acids share close structural similarity with alk(en)yl citraconate derivatives, chaetomellic acids and other lichen lactones, protolichesterinic, lichesterinic, and murolic acids. 相似文献
98.
99.
100.