全文获取类型
收费全文 | 3299篇 |
免费 | 220篇 |
专业分类
3519篇 |
出版年
2023年 | 9篇 |
2022年 | 26篇 |
2021年 | 31篇 |
2020年 | 23篇 |
2019年 | 32篇 |
2018年 | 45篇 |
2017年 | 43篇 |
2016年 | 74篇 |
2015年 | 88篇 |
2014年 | 101篇 |
2013年 | 205篇 |
2012年 | 172篇 |
2011年 | 179篇 |
2010年 | 109篇 |
2009年 | 96篇 |
2008年 | 194篇 |
2007年 | 167篇 |
2006年 | 181篇 |
2005年 | 198篇 |
2004年 | 180篇 |
2003年 | 168篇 |
2002年 | 175篇 |
2001年 | 92篇 |
2000年 | 82篇 |
1999年 | 83篇 |
1998年 | 40篇 |
1997年 | 50篇 |
1996年 | 38篇 |
1995年 | 42篇 |
1994年 | 41篇 |
1993年 | 30篇 |
1992年 | 53篇 |
1991年 | 47篇 |
1990年 | 42篇 |
1989年 | 58篇 |
1988年 | 46篇 |
1987年 | 22篇 |
1986年 | 32篇 |
1985年 | 24篇 |
1984年 | 22篇 |
1983年 | 13篇 |
1982年 | 12篇 |
1981年 | 20篇 |
1980年 | 9篇 |
1979年 | 15篇 |
1978年 | 15篇 |
1977年 | 9篇 |
1976年 | 11篇 |
1975年 | 14篇 |
1971年 | 10篇 |
排序方式: 共有3519条查询结果,搜索用时 15 毫秒
101.
102.
Fusao Tomita Hirofumi Nakano Haruo Honda Takeo Suzuki 《Bioscience, biotechnology, and biochemistry》2013,77(11):2183-2188
The isolation of chloramphenicol resistant strains from Corynebacterium hydrocarboclastus KY 4339 (rough type) was examined to seek a good source of corynecins (analogs of chloramphenicol). Various mutants resistant to chloramphenicol were isolated in the range from 50 to 1000 µg/ml by adaptation or induced mutagenesis by N-methyl-N′-nitro-N-nitro-soguanidine. Productivities of mutants related apparently to the degree of resistance from 50 to 500 µg/ml. Highly resistant mutants capable of growing in the presence of 1000 µg of chloramphenicol per ml showed decreased productivity which might be related to their lower growth rate in the fermentation medium.Further attempts to derive resistant mutants to structural analogs of aromatic amino acids resulted in only a slight improvement of productivity, indicating that aromatic amino acids might play minor regulatory roles in corynecins synthesis.The increase in productivity of corynecins by the best strain was about 4.5 fold of the parental strain. 相似文献
103.
Isao Shibuya Hiroko Honda Bunji Maruo 《Bioscience, biotechnology, and biochemistry》2013,77(1):111-114
An NAD linked formate dehydrogenating enzyme which catalyzed the last step of methanol oxidation system was extracted from the methanol-grown Kloeckera sp. No. 2201. The specific activity of the enzyme in the extract of methanol-grown cells was found to be considerably higher than that of the glucose-grown cells. The enzyme was purified about 35-fold from the extract of methanol-grown cells by heat treatment, column chromatographies on DEAE-cellulose and on hydroxylapatite, and Sephadex G-200 gel filtration. The purified enzyme was shown to be homogeneous by analyses with electrophoresis and ultracentrifuga-tion. The purified enzyme was a kind of NAD: formate oxidoreductase (EC, 1.2.1.2) which catalyzed specifically the oxidation of formate to carbon dioxide. The Km values were 22 mm for formate and 0.1 mm for NAD. The enzyme was inactivated by potassium cyanide, sodium azide, and p-chloromercuribenzoate but not by any metal-chelating reagents tested. Other general properties of the enzyme were also investigated. 相似文献
104.
Yoichi Ozawa Kenji Mori Masanao Matsui 《Bioscience, biotechnology, and biochemistry》2013,77(7):1383-1386
Thirty eight new compounds with juvenile hormone (JH) activity were synthesized and evaluated biologically on Bombyx mori L. Among them, the activities of 7,8-epoxy-4,8-dimethyl-1 -(p-ethylphenoxy)-3-undecene and 7,8-epoxy-4,8-dimethyl-l-(p-methylphenoxy)-3-undencene were proved to be more than two thousand times as high as that of the C18-Cecropia JH (mixed isomers). Structure-activity relationship of these compounds was discussed. 相似文献
105.
Masanao Matsui Kenji Mori Yoichi Ozawa 《Bioscience, biotechnology, and biochemistry》2013,77(2):193-195
A synthesis of oospolactone (3,4-dimethyl-8-hydroxyisocoumarin), a metabolite of Oospora astringenes, is described. 相似文献
106.
Kazunori Sasaki Tomoyuki Oki Yumi Kai Yoichi Nishiba 《Bioscience, biotechnology, and biochemistry》2013,77(8):1308-1314
The purpose of this study was to investigate the effect of repeated harvesting on the content of caffeic acid (CA) and seven species of caffeoylquinic acids (CQAs) in sweet potato leaves using a newly developed high-performance liquid chromatography method. Six cultivars and two breeding lines were used in this study. Leaves were collected at monthly intervals from 1st harvest (May) to 4th harvest (August) in 2011 and 2012. ANOVA analysis revealed that the contents of CQAs were significantly different among all cultivars and breeding lines, but no significant differences were found for CA. No annual variation was confirmed in CA and CQAs. Repeated harvest of sweet potato leaves affected the content of only 4-CQA and 5-CQA. Post-hoc comparisons using Tukey’s method indicated that the contents of 4-CQA and 5-CQA in sweet potato leaves harvested at first time were significantly higher compared to those at the other harvest times. 相似文献
107.
Yoichi Ozawa Kenji Mori Masanao Matsui 《Bioscience, biotechnology, and biochemistry》2013,77(10):2373-2378
Sixteen new Cecropia juvenile hormone (JH) analogs with different alkyl substituents at C–7 and C–11 were synthesized as stereoisomeric mixtures. The epoxides with n-propyl or n-butyl and methyl groups at C-11 and methyl or ethyl group at C-7 showed high JH activity on Bombyx mori L. Structure-activity relationship of the JH analogs was discussed. 相似文献
108.
Kyoung Ho Lee Tomomi Tsutsui Kohsuke Honda Hisao Ohtake Takeshi Omasa 《Cytotechnology》2013,65(6):1017-1026
The effects of mutant cell division cycle 25 homolog B (CDC25B) overexpression on the generation of cells producing a monoclonal antibody were investigated in Chinese hamster ovary (CHO) cells. Mutant CDC25B (m-CDC25B) expression plasmids were transfected into CHO DG44-derived cells producing a monoclonal antibody, and the frequency of highly producing cells was assessed following gene amplification in the presence of 250 nM methotrexate. Most of the clones obtained from the m-CDC25B-overexpressing cells had higher antibody titers than did mock-transfected control cells. This arose from either higher transgene copy numbers or higher mRNA expression levels for the antibody. However, the high mRNA expression levels were not always accompanied by increases in transgene copy numbers. Our results suggest that cells producing high levels of a monoclonal antibody can be selected efficiently using m-CDC25B overexpression. 相似文献
109.
110.
Hirokazu Honda Yasuna Kobayashi Shoko Onuma Keigo Shibagaki Toshitaka Yuza Keiichi Hirao Toshinori Yamamoto Naohisa Tomosugi Takanori Shibata 《PloS one》2016,11(3)