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101.
Yuko Miyajima-Taga Reiji Masuda Yoh Yamashita 《Environmental Biology of Fishes》2017,100(10):1237-1249
Although many fish species are known to feed on jellyfish, only a few quantitative studies on this subject have been conducted in the field or laboratory. We combined field observations and feeding experiments to quantitatively evaluate the potential feeding capability of the black scraper Thamnaconus modestus on giant jellyfish Nemopilema nomurai in a jellyfish-abundant area. Underwater observations revealed that shoals of T. modestus fed voraciously on jellyfish when the jellyfish were close to the fish’s seaweed habitat. Gut contents of field-collected T. modestus almost exclusively consisted of jellyfish. In tank trials, feeding activity of black scraper was high when light intensity was between about 1.1 lx and 50 × 103 lx. The estimated feeding speed of T. modestus on the jellyfish in the Sea of Japan when the jellyfish bloom occurred was 10.0 ± 2.0 times fish body weight per day. The results support the prediction that T. modestus and probably other coastal medusivorous fishes have a substantial capability to eliminate jellyfish blooms. Considering that they are highly dependent on seaweed bed, protection of such habitats for these medusivores may be the most cost-efficient control method for jellyfish blooms. 相似文献
102.
Salmonella bacteria cause more than three million deaths each year. They hijack cells and inject among other proteins SipA via a "molecular syringe" into the cell, which can tether actin subunits in opposing strands to form mechanically stabilized filaments which rapidly reshape the cells surface into extended ruffles, leading to bacterial internalization. Exactly how these ruffles form at a single filament level remains unknown. Our real time total internal fluorescence microscopy observations show that both bidirectional elongation of actin by SipA as well as end-to-end annealing of SipA-actin filaments are rapid processes. Complementary electron microscopy investigations demonstrate that crowding agents in vitro readily induce stiff bundles of SipA-actin filaments. Taken together these three effects, rapid SipA induced actin polymerization, filament annealing and bundle formation due to molecular crowding can explain how Salmonella invades cells at molecular level. 相似文献
103.
David Popp Mitsusada Iwasa Akihiro Narita Harold P. Erickson Yuichiro Maéda 《Biopolymers》2009,91(5):340-350
In vivo cell division protein FtsZ from E. coli forms rings and spirals which have only been observed by low resolution light microscopy. We show that these suprastructures are likely formed by molecular crowding which is a predominant factor in prokaryotic cells and enhances the weak lateral bonds between proto‐filaments. Although FtsZ assembles into single proto‐filaments in dilute aqueous buffer, with crowding agents above a critical concentration, it forms polymorphic supramolecular structures including rings and toroids (with multiple protofilaments) about 200 nm in diameter, similar in appearance to DNA toroids, and helices with pitches of several hundred nm as well as long, linear bundles. Helices resemble those observed in vivo, whereas the rings and toroids may represent a novel energy minimized state of FtsZ, at a later stage of Z‐ring constriction. We shed light on the molecular arrangement of FtsZ filaments within these suprastructures using high resolution electron microscopy. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 340–350, 2009. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com 相似文献
104.
Sachiko Saino-Saito Reza Mohammad Nourani Hiroo Iwasa Hisatake Kondo Yuji Owada 《Cell and tissue research》2009,338(2):191-201
The analysis of myenteric neurons is becoming increasingly important for the assessment of enteric nervous system injury and
degeneration occurring in motor disorders of the gut. Limited information is presently available on the quantitative estimation
of myenteric neurons and glial cells in paraffin-embedded colonic sections; additional data would be useful for diagnostic
purposes. In this morphometric study, we performed immunohistochemistry to count myenteric neurons and glial cells in paraffin
sections of human colon. Serial cross sections of formalin-fixed paraffin-embedded full-thickness normal human left colon
(n = 10, age-range: 50–72 years) were examined. HuC/D and S100β antigens were found to be the best markers for the detection
of neurons and glial cells, respectively. Significant correlations were noted between the numbers of neurons/glial cells and
the respective myenteric ganglion areas. These findings suggest that HuC/D-S100β-immunostained paraffin cross sections of
human colon can be regarded as valuable tools for the quantitative estimation of myenteric neurons and glial cells. Based
on the present method, only a limited number of paraffin sections are needed for reliable quantitative assessments of myenteric
ganglion cells, thus allowing fast and simple approaches in the settings of the histopathological diagnosis of colonic motility
disorders and retrospective evaluations of pathological archival tissue specimens.
R. De Giorgio is the recipient of grants from the Fondazione Del Monte di Bologna e Ravenna and from the Fondazione Cassa
di Risparmio, Bologna, Italy. The authors declare no conflicting interests. 相似文献
105.
Shiratsuchi A Ichiki M Okamoto Y Ueda N Sugimoto N Takuwa Y Nakanishi Y 《Journal of biochemistry》2009,145(1):43-50
The production of N-acylethanolamine (NAE) is enhanced during inflammation. NAE is synthesized from phosphatidylethanolamine with N-acylphosphatidylethanolamine (NAPE) as a precursor. The amount of NAPE at the site of inflammation exceeds that of NAE. This evokes the possibility that NAPE possesses a biological function, as does NAE. We here examined if N-palmitoylphosphatidylethanolamine (NPPE), a precursor of N-palmitoylethanolamine, modulates the state of inflammation. We found that the level of the phagocytosis of latex beads, Staphylococcus aureus, Escherichia coli, or apoptotic cells by mouse peritoneal macrophages or J774A.1 macrophages was reduced in the presence of liposomes containing NPPE, while that of dextran remained unaffected. This action of NPPE seemed to be due to the inhibition of the activation of Rac1 and Cdc42 in macrophages. These results suggested that NAPE is bioactive lipid acting toward the termination of inflammation. 相似文献
106.
Progressing waves of regeneration are observed in forest ecosystems such as Shimagare fir forests. The patterns generated by lattice models for forest dynamics often show similar waves of disturbance and recovery. This paper introduces a method to detect and quantify the directional movement of these waves. The method is based only on the disturbance times of the sites and allows to distinguish three types of wave patterns: patterns with global direction, patterns with local direction, and patterns without direction. We apply this to several grid-based models for forest dynamics which are evaluated analytically or by simulation. The results reveal a clear distinction of the models which earlier studies were not able to detect. 相似文献
107.
Background
Vacuolar-type proton transporting ATPase (V-ATPase) is involved in the proper development of visual function. Mutations in the Tcirg1 (also known as Atp6V0a3) locus, which encodes the a3 subunit of V-ATPase, cause severe autosomal recessive osteopetrosis (ARO) in humans. ARO is often associated with impaired vision most likely because of nerve compression at the optic canal. We examined the ocular phenotype of mice deficient in Tcirg1 function.Methodology/Principal Findings
X-ray microtomography showed narrowed foramina in the skull, suggesting that optic nerve compression occurred in the a3-deficient (Tcirg1 −/−) mice. The retina of the mutant mice had normal architecture, but the number of apoptotic cells was increased at 2–3 wks after birth. In the ocular system, the a3 subunit accumulated in the choriocapillary meshwork in uveal tissues. Two other subunit isoforms a1 and a2 accumulated in the retinal photoreceptor layer. We found that the a4 subunit, whose expression has previously been shown to be restricted to several transporting epithelia, was enriched in pigmented epithelial cells of the retina and ciliary bodies. The expression of a4 in the uveal tissue was below the level of detection in wild-type mice, but it was increased in the mutant choriocapillary meshwork, suggesting that compensation may have occurred among the a subunit isoforms in the mutant tissues.Conclusions
Our findings suggest that a similar etiology of visual impairment is involved in both humans and mice; thus, a3-deficient mice may provide a suitable model for clinical and diagnostic purposes in cases of ARO. 相似文献108.
Md.?Shahidul?IslamEmail author Yoh?Yamashita Masaru?Tanaka 《Environmental Biology of Fishes》2011,91(4):389-405
Recruitment in marine fishes is regulated largely by the demographic changes that occur during the early life stages; therefore,
a thorough understanding of early life stages is essential for predicting recruitment variability in fishes. Japanese sea
bass (JSB), Lateolabrax japonicus, is a coastal marine fish distributed in East Asian coastal waters, and is regarded as highly important for commercial and
recreational fisheries, for marine and brackish water aquaculture as well as for stock enhancement. JSB is a typical estuarine
dependent temperate fish, which spawns in shelf areas and coastal embayments and the larvae and juveniles are dispersed and
transported into shallow nearshore habitats and estuaries where they spend the early life. In this paper, we provide insight
into the early life history and ecology of JSB through a revision of the available information and using the data we obtained
from a relatively long-term research. We review and discuss the distribution and habitat use, food and feeding, age and growth,
mortality and recruitment of larval and juvenile JSB in coastal waters around Japan. We extend our discussions in all available
dimensions: habitat-specific, ontogenetic, and spatio-temporal, and highlight the importance of nursery habitats. We also
discuss the implications of early life history for recruitment of JSB as well as the possible effects of climate change. At
the end, we point out potential areas for future research. 相似文献
109.
The mitochondrial DNA phylogeographical structure of the freshwater atyid shrimp Neocaridina denticulata denticulata was investigated near the Yura and Kako Rivers in western Japan. To assess the biological significance of drainage evolution, fragments of 390 base pairs (bp) extending from the NADH dehydrogenase subunit 2 gene to the tryptophan transfer RNA gene were sequenced for 246 specimens from twenty locations. The phylogenetic tree detected two distinct clades corresponding to the region along the Sea of Japan and Seto Inland Sea, respectively. Geographical mapping of the two clades well reflected the river capture, in which the upper reaches of the Yura River draining into the Sea of Japan had been captured from the Kako River into the Seto Inland Sea. The clear pattern of mitochondrial DNA distribution may be accounted for by the ecological characteristics of species that minimize passive downstream drift, local adaptation, and mountain topography. 相似文献
110.