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101.
We modelled the population dynamics of two types of plants with limited dispersal living in a lattice structured habitat.
Each site of the square lattice model was either occupied by an individual or vacant. Each individual reproduced to its neighbors.
We derived a criterion for the invasion of a rare type into a population composed of a resident type based on a pair-approximation
method, in which the dynamics of both average densities and the nearest neighbor correlations were considered. Based on this
invasibility criterion, we showed that, when there is a tradeoff between birth and death rates, the evolutionarily stable
type is the one that has the highest ratio of birth rate to mortality. If these types are different species, they form segregated
spatial patterns in the lattice model in which intraspecific competitive interactions occur more frequently than interspecific
interactions. However, stable coexistence is not possible in the lattice model contrary to results from completely mixed population
models. This clearly shows that the casual conclusion, based on traditional well mixed population models, that different species
can coexist if intraspecific competition is stronger than interspecific competition, does not hold for spatially structured
population models. 相似文献
102.
103.
M Yoh E K Frimpong S P Voravuthikunchai T Honda 《Canadian journal of microbiology》1999,45(9):732-739
In Japan, antimicrobial agent therapy for patients with diarrhea due to enterovirulent organisms including enterohemorrhagic Escherichia coli (EHEC) is common, and norfloxacin (NFLX), fosfomycin, and kanamycin are recommended for EHEC treatment by the Japanese Ministry of Health and Welfare. The aim of this study was to analyze the effects of antimicrobial agents which have been used or recommended for the treatment of EHEC on the production of verotoxin (VT) in vitro. Subinhibitory concentrations of quinolones, NFLX, sparofloxacin (SPFX), and grepafloxacin (GPFX) markedly stimulated the productions of VT1 and VT2. The macrolide azithromycin (AZM), erythromycin (EM), and clarithromycin (CAM) did not stimulate the production of VT at a wide range of concentrations. These in vitro results indicate that when quinolones are prescribed for a patient infected with EHEC, the concentration of antimicrobial agents used in vivo and the susceptibility of the EHEC strains against quinolones should be taken into consideration. 相似文献
104.
S Kondo S Toyokuni Y Iwasa T Tanaka H Onodera H Hiai M Imamura 《Free radical biology & medicine》1999,27(3-4):401-410
Few studies have been conducted focusing on a potential role of reactive oxygen species in tumor cell metabolism. Here we studied human colorectal adenocarcinomas and adenomas to determine whether oxidative stress is imposed on cancer cells in vivo and used specific antibodies against 8-hydroxy-2'-deoxyguanosine (8-OHdG), 4-hydroxy-2-nonenal (HNE)-modified proteins, and 3-nitro-L-tyrosine (3-NT) to determine whether there is an association between oxidative stress and cellular proliferation. Higher levels of oxidative modifications in DNA and proteins were observed in carcinoma cells, but not in adenoma cells, than in the corresponding nontumorous epithelial cells by immunohistochemistry as well as high-performance liquid chromatography (HPLC)-based 8-OHdG determination. The fraction of proliferating cell nuclear antigen-positive cells was proportionally associated in adenocarcinomas with the staining intensities of 8-OHdG and 3-NT. Furthermore, Western blot analysis of the proteins extracted from carcinoma cells revealed several specific proteins modified by HNE or peroxynitrite. Thus we concluded that colorectal carcinoma, but not adenoma cells, are exposed to more oxidative stress than their corresponding nontumorous epithelial cells, regardless of clinical stage and histology, and further that the oxidative stress in carcinoma cells might stimulate cellular proliferation. 相似文献
105.
Yuko Miyajima-Taga Reiji Masuda Yoh Yamashita 《Environmental Biology of Fishes》2017,100(10):1237-1249
Although many fish species are known to feed on jellyfish, only a few quantitative studies on this subject have been conducted in the field or laboratory. We combined field observations and feeding experiments to quantitatively evaluate the potential feeding capability of the black scraper Thamnaconus modestus on giant jellyfish Nemopilema nomurai in a jellyfish-abundant area. Underwater observations revealed that shoals of T. modestus fed voraciously on jellyfish when the jellyfish were close to the fish’s seaweed habitat. Gut contents of field-collected T. modestus almost exclusively consisted of jellyfish. In tank trials, feeding activity of black scraper was high when light intensity was between about 1.1 lx and 50 × 103 lx. The estimated feeding speed of T. modestus on the jellyfish in the Sea of Japan when the jellyfish bloom occurred was 10.0 ± 2.0 times fish body weight per day. The results support the prediction that T. modestus and probably other coastal medusivorous fishes have a substantial capability to eliminate jellyfish blooms. Considering that they are highly dependent on seaweed bed, protection of such habitats for these medusivores may be the most cost-efficient control method for jellyfish blooms. 相似文献
106.
Salmonella bacteria cause more than three million deaths each year. They hijack cells and inject among other proteins SipA via a "molecular syringe" into the cell, which can tether actin subunits in opposing strands to form mechanically stabilized filaments which rapidly reshape the cells surface into extended ruffles, leading to bacterial internalization. Exactly how these ruffles form at a single filament level remains unknown. Our real time total internal fluorescence microscopy observations show that both bidirectional elongation of actin by SipA as well as end-to-end annealing of SipA-actin filaments are rapid processes. Complementary electron microscopy investigations demonstrate that crowding agents in vitro readily induce stiff bundles of SipA-actin filaments. Taken together these three effects, rapid SipA induced actin polymerization, filament annealing and bundle formation due to molecular crowding can explain how Salmonella invades cells at molecular level. 相似文献
107.
David Popp Mitsusada Iwasa Akihiro Narita Harold P. Erickson Yuichiro Maéda 《Biopolymers》2009,91(5):340-350
In vivo cell division protein FtsZ from E. coli forms rings and spirals which have only been observed by low resolution light microscopy. We show that these suprastructures are likely formed by molecular crowding which is a predominant factor in prokaryotic cells and enhances the weak lateral bonds between proto‐filaments. Although FtsZ assembles into single proto‐filaments in dilute aqueous buffer, with crowding agents above a critical concentration, it forms polymorphic supramolecular structures including rings and toroids (with multiple protofilaments) about 200 nm in diameter, similar in appearance to DNA toroids, and helices with pitches of several hundred nm as well as long, linear bundles. Helices resemble those observed in vivo, whereas the rings and toroids may represent a novel energy minimized state of FtsZ, at a later stage of Z‐ring constriction. We shed light on the molecular arrangement of FtsZ filaments within these suprastructures using high resolution electron microscopy. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 340–350, 2009. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com 相似文献
108.
Sachiko Saino-Saito Reza Mohammad Nourani Hiroo Iwasa Hisatake Kondo Yuji Owada 《Cell and tissue research》2009,338(2):191-201
The analysis of myenteric neurons is becoming increasingly important for the assessment of enteric nervous system injury and
degeneration occurring in motor disorders of the gut. Limited information is presently available on the quantitative estimation
of myenteric neurons and glial cells in paraffin-embedded colonic sections; additional data would be useful for diagnostic
purposes. In this morphometric study, we performed immunohistochemistry to count myenteric neurons and glial cells in paraffin
sections of human colon. Serial cross sections of formalin-fixed paraffin-embedded full-thickness normal human left colon
(n = 10, age-range: 50–72 years) were examined. HuC/D and S100β antigens were found to be the best markers for the detection
of neurons and glial cells, respectively. Significant correlations were noted between the numbers of neurons/glial cells and
the respective myenteric ganglion areas. These findings suggest that HuC/D-S100β-immunostained paraffin cross sections of
human colon can be regarded as valuable tools for the quantitative estimation of myenteric neurons and glial cells. Based
on the present method, only a limited number of paraffin sections are needed for reliable quantitative assessments of myenteric
ganglion cells, thus allowing fast and simple approaches in the settings of the histopathological diagnosis of colonic motility
disorders and retrospective evaluations of pathological archival tissue specimens.
R. De Giorgio is the recipient of grants from the Fondazione Del Monte di Bologna e Ravenna and from the Fondazione Cassa
di Risparmio, Bologna, Italy. The authors declare no conflicting interests. 相似文献
109.
Shiratsuchi A Ichiki M Okamoto Y Ueda N Sugimoto N Takuwa Y Nakanishi Y 《Journal of biochemistry》2009,145(1):43-50
The production of N-acylethanolamine (NAE) is enhanced during inflammation. NAE is synthesized from phosphatidylethanolamine with N-acylphosphatidylethanolamine (NAPE) as a precursor. The amount of NAPE at the site of inflammation exceeds that of NAE. This evokes the possibility that NAPE possesses a biological function, as does NAE. We here examined if N-palmitoylphosphatidylethanolamine (NPPE), a precursor of N-palmitoylethanolamine, modulates the state of inflammation. We found that the level of the phagocytosis of latex beads, Staphylococcus aureus, Escherichia coli, or apoptotic cells by mouse peritoneal macrophages or J774A.1 macrophages was reduced in the presence of liposomes containing NPPE, while that of dextran remained unaffected. This action of NPPE seemed to be due to the inhibition of the activation of Rac1 and Cdc42 in macrophages. These results suggested that NAPE is bioactive lipid acting toward the termination of inflammation. 相似文献
110.
Progressing waves of regeneration are observed in forest ecosystems such as Shimagare fir forests. The patterns generated by lattice models for forest dynamics often show similar waves of disturbance and recovery. This paper introduces a method to detect and quantify the directional movement of these waves. The method is based only on the disturbance times of the sites and allows to distinguish three types of wave patterns: patterns with global direction, patterns with local direction, and patterns without direction. We apply this to several grid-based models for forest dynamics which are evaluated analytically or by simulation. The results reveal a clear distinction of the models which earlier studies were not able to detect. 相似文献