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61.
Joshi AA Kanekar PP Kelkar AS Shouche YS Vani AA Borgave SB Sarnaik SS 《Microbial ecology》2008,55(2):163-172
Aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season,
January 2002 from alkaline Lonar lake, India, having pH 10.5. The total number of microorganisms in the sediment and water
samples was found to be 102–106 cfu g−1 and 102–104 cfu ml−1, respectively. One hundred and ninety-six strains were isolated using different enrichment media. To study the bacterial
diversity of Lonar lake and to select the bacterial strains for further characterization, screening was done on the basis
of pH and salt tolerance of the isolates. Sixty-four isolates were subjected to phenotypic, biochemical characterization and
16S rRNA sequencing. Out of 64, 31 bacterial isolates were selected on the basis of their enzyme profile and further subjected
to phylogenetic analysis. Phylogenetic analysis indicated that most of the Lonar lake isolates were related to the phylum
Firmicutes, containing Low G+C, Gram-positive bacteria, with different genera: Bacillus, Paenibacillus, Alkalibacillus, Exiguobacterium, Planococcus, Enterococcus and Vagococcus. Seven strains constituted a Gram-negative bacterial group, with different genera: Halomonas, Stenotrophomonas and Providencia affiliated to γ-Proteobacteria, Alcaligenes to β-Proteobacteria and Paracoccus to α-Proteobacteria. Only five isolates were High G+C, Gram-positive bacteria associated with phylum Actinobacteria, with
various genera: Cellulosimicrobium, Dietzia, Arthrobacter and Micrococcus. Despite the alkaline pH of the Lonar lake, most of the strains were alkalitolerant and only two strains were obligate alkaliphilic.
Most of the isolates produced biotechnologically important enzymes at alkaline pH, while only two isolates (ARI 351 and ARI
341) showed the presence of polyhydroxyalkcanoate (PHA) and exopolysaccharide (EPS), respectively. 相似文献
62.
Alioua A Lu R Kumar Y Eghbali M Kundu P Toro L Stefani E 《The Journal of biological chemistry》2008,283(8):4808-4817
The large conductance, voltage- and Ca2+-activated potassium (MaxiK, BK) channel and caveolin-1 play important roles in regulating vascular contractility. Here, we hypothesized that the MaxiK alpha-subunit (Slo1) and caveolin-1 may interact with each other. Slo1 and caveolin-1 physiological association in native vascular tissue is strongly supported by (i) detergent-free purification of caveolin-1-rich domains demonstrating a pool of aortic Slo1 co-migrating with caveolin-1 to light density sucrose fractions, (ii) reverse co-immunoprecipitation, and (iii) double immunolabeling of freshly isolated myocytes revealing caveolin-1 and Slo1 proximity at the plasmalemma. In HEK293T cells, Slo1-caveolin-1 association was unaffected by the smooth muscle MaxiK beta1-subunit. Sequence analysis revealed two potential caveolin-binding motifs along the Slo1 C terminus, one equivalent, 1007YNMLCFGIY1015, and another mirror image, 537YTEYLSSAF545, to the consensus sequence, varphiXXXXvarphiXXvarphi. Deletion of 1007YNMLCFGIY1015 caused approximately 80% loss of Slo1-caveolin-1 association while preserving channel normal folding and overall Slo1 and caveolin-1 intracellular distribution patterns. 537YTEYLSSAF545 deletion had an insignificant dissociative effect. Interestingly, caveolin-1 coexpression reduced Slo1 surface and functional expression near 70% without affecting channel voltage sensitivity, and deletion of 1007YNMLCFGIY1015 motif obliterated channel surface expression. The results suggest 1007YNMLCFGIY1015 possible participation in Slo1 plasmalemmal targeting and demonstrate its role as a main mechanism for caveolin-1 association with Slo1 potentially serving a dual role: (i) maintaining channels in intracellular compartments downsizing their surface expression and/or (ii) serving as anchor of plasma membrane resident channels to caveolin-1-rich membranes. Because the caveolin-1 scaffolding domain is juxtamembrane, it is tempting to suggest that Slo1-caveolin-1 interaction facilitates the tethering of the Slo1 C-terminal end to the membrane. 相似文献
63.
Chakravarthy VS Gupte N Yogesh S Salhotra A 《International journal of neural systems》2008,18(2):157-164
Synchronization of chaotic low-dimensional systems has been a topic of much recent research. Such systems have found applications for secure communications. In this work we show how synchronization can be achieved in a high-dimensional chaotic neural network. The network used in our studies is an extension of the Hopfield Network, known as the Complex Hopfield Network (CHN). The CHN, also an associative memory, has both fixed point and limit cycle or oscillatory behavior. In the oscillatory mode, the network wanders chaotically from one stored pattern to another. We show how a pair of identical high-dimensional CHNs can be synchronized by communicating only a subset of state vector components. The synchronizability of such a system is characterized through simulations. 相似文献
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