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301.
Min Soo Kim Gyoung Mi Kim Yun-Jeong Choi Hye Joung Kim Yoo-Jin Kim Wook Jin 《Molecules and cells》2013,36(2):177-184
It has been suggested that activation of receptor PTKs is important for leukemogenesis and leukemia cell response to targeted therapy in hematological malignancies including leukemia. PTKs induce activation of the PI3K/Akt/mTOR pathway, which can result in prevention of apoptosis. Here, we describe an important role of the TrkC-associated molecular network in the process of leukemogenesis. TrkC was found to be frequently overexpressed in human leukemia cells and leukemia subtypes. In U937 human leukemia cells, blockade of TrkC using small hairpin RNA (shRNA) specific to TrkC or K562a, a specific inhibitor of TrkC, resulted in a significant decrease in growth and survival of the cells, which was closely associated with reduced mTOR level and Akt activity. In addition, TrkC enhances the survival and proliferation of leukemia, which is correlated with activation of the PI3K/Akt pathway. Moreover, TrkC significantly inhibits apoptosis via induction of the expression of PLK-1 and Twist-1 through activation of AKT/mTor pathway; therefore, it plays a key role in leukemogenesis. These findings reveal an unexpected physiological role for TrkC in the pathogenesis of leukemia and have important implications for understanding various hematological malignancies. 相似文献
302.
Chi-Yong Ahn Seung-Hyun Joung Chan-Sun Park Hee-Sik Kim Byung-Dae Yoon Hee-Mock Oh 《Hydrobiologia》2008,596(1):413-421
Different sampling methods can often produce different results when cyanobacterial blooms are intense and there are surface
scums. Accordingly, five commonly used sampling methods and four sampling times were compared for monitoring of Oscillatoria and Microcystis populations. The different methods and times led to significantly different results for cyanobacterial biomass and its variance.
Sampling methods that tended to be more specific to surface scums resulted in much higher and more variable measurements.
Although sampling later in the day greatly reduced variances, the measured biomass was much lower than at earlier times of
day, which was attributed to cyanobacterial downward migration in the afternoon. These trends were common for Oscillatoria and Microcystis. In order to overcome such problems, the median and median absolute deviation (MAD) were proposed, instead of the arithmetic
mean and standard deviation (SD), for presenting a central tendency of measured values. Although the mean ± SD has been widely
used to express a central tendency, it is too sensitive to outlying values that are very common during cyanobacterial blooms.
The large differences in the mean values between the sampling methods and times were reduced by using the median values. Furthermore,
the median ± MAD revealed the real data distribution more effectively.
Handling editor: D. Hamilton 相似文献
303.
Hee Y. Paik Hyojee Joung Joo Y. Lee Hong K. Lee Janet C. King Carl L. Keen 《Biological trace element research》1999,69(1):45-57
The present study focused on whether serum extracellular superoxide dimutase (EC-SOD) activity can be used as a functional
indicator of marginal zinc deficiency in humans. Subjects in this study were 444 healthy adults over 30 yr of age living a
normal rural life in Kyunggi province, Korea. The mean dietary zinc intake of subjects obtained from one 24-h recall was 6.41
± 4.35 mg and the average serum zinc concentration of the subjects was 11.06 ± 2.44 (μmol/L. Subjects were divided into three
groups by serum zinc concentrations: adequate (serum zinc >10.7 (μmol/L), low (serum zinc 9.0–10.7 μmol/L), and very low (serum
zinc <9.0 μmol/L) groups. A total of 50 subjects were selected from the three groups for analysis of EC-SOD activities. The
EC-SOD activity of subjects increased with increasing serum zinc concentrations, and the activities of the three groups were
significantly different as indicated by the Kruskal-Wallis test (p = 0.0239). Also, serum EC-SOD activities were significantly
correlated with serum zinc concentrations (r = 0.289,p = 0.04). Serum EC-SOD activities, however, were not significantly correlated to the dietary zinc intakes. In conclusion,
these results show that EC-SOD activities are decreased in subjects with low serum zinc concentrations and suggest that EC-SOD
activity may be a functional indicator of zinc nutritional status in humans. 相似文献
304.
Jihye Kim Hee Young Paik Hyojee Joung Leslie R. Woodhouse Janet C. King 《Biological trace element research》2011,142(2):130-136
This study was done to determine the effect of age on zinc metabolism and status among healthy Korean women. Measures of zinc
metabolism and status were measured in eight young women (22–24 years) and seven elderly women (66–75 years) consuming a typical
Korean diet. Oral and intravenous tracers highly enriched in 67Zn and 70Zn were administered simultaneously. Multiple plasma, 24-h urines, and fecal samples were collected after isotope administration.
In the young women, additional plasma were collected to determine zinc kinetics using a seven-compartmental model. Exchangeable
Zinc Pool (EZP) was estimated by Miller’s method. Plasma zinc concentrations were higher in older women than younger women
(p < 0.05). EZP and urinary zinc tended to be higher in older women than younger women. Fractional and total zinc absorption
and endogenous fecal zinc losses did not differ between young and older women. A comparison of the zinc kinetics of the Korean
and American women showed no differences in plasma or EZP zinc parameters. However, absorbed zinc and zinc flux to slowly
turning over tissues (Q7) were lower in Korean women than that of Americans (p < 0.01) suggesting the total body zinc content of Korean women is lower than that of American women. 相似文献
305.
306.
307.
Kuy‐Sook Lee Jin‐Hee Park Seahyoung Lee Hyun‐Joung Lim Hyun‐Young Park 《Journal of cellular biochemistry》2009,106(5):823-834
Peroxisome proliferator‐activated receptors δ (PPARδ) is known to be expressed ubiquitously, and the predominant PPAR subtype of cardiac cells. However, relatively less is known regarding the role of PPARδ in cardiac cells except that PPARδ ligand treatment protects cardiac hypertrophy by inhibiting NF‐κB activation. Thus, in the present study, we examined the effect of selective PPARδ ligand L‐165041 on angiotensin II (AngII) induced cardiac hypertrophy and its underlying mechanism using cardiomyocyte. According to our data, L‐165041 (10 µM) inhibited AngII‐induced [3H] leucine incorporation, induction of the fetal gene atrial natriuretic factor (ANF) and increase of cardiomyocyte size. Previous studies have implicated the activation of focal adhesion kinase (FAK) in the progress of cardiomyocyte hypertrophy. L‐165041 pretreatment significantly inhibited AngII‐induced intracellular Ca2+ increase and subsequent phosphorylation of FAK. Further experiment using Ca2+ ionophore A23187 confirmed that Ca2+ induced FAK phosphorylation, and this was also blocked by L‐165041 pretreatment. In addition, overexpression of PPARδ using adenovirus significantly inhibited AngII‐induced intracellular Ca2+ increase and FAK expression, while PPARδ siRNA treatment abolished the effect of L‐165041. These data indicate that PPARδ ligand L‐165041 inhibits AngII induced cardiac hypertrophy by suppressing intracellular Ca2+/FAK/ERK signaling pathway in a PPARδ dependent mechanism. J. Cell. Biochem. 106: 823–834, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
308.
On 27 October 2013, a Rhincodon typus was apparently chased by a group of Caranx ignobilis into nearshore waters near Green Island (Ludao), east of Taiwan. A fisherman brought it back to port where it was kept in a small sea pen until release. The R. typus was 78 cm total length, and was tagged and released on 29 October 2013. 相似文献
309.
Dawoon Baek Kwang Hwan Park Kyoung-Mi Lee Sujin Jung Soyeong Joung Jihyun Kim Jin Woo Lee 《Cell death & disease》2021,12(3)
The ubiquitin protease pathway plays important role in human bone marrow-derived mesenchymal stem cell (hBMSC) differentiation, including osteogenesis. However, the function of deubiquitinating enzymes in osteogenic differentiation of hBMSCs remains poorly understood. In this study, we aimed to investigate the role of ubiquitin-specific protease 53 (USP53) in the osteogenic differentiation of hBMSCs. Based on re-analysis of the Gene Expression Omnibus database, USP53 was selected as a positive regulator of osteogenic differentiation in hBMSCs. Overexpression of USP53 by lentivirus enhanced osteogenesis in hBMSCs, whereas knockdown of USP53 by lentivirus inhibited osteogenesis in hBMSCs. In addition, USP53 overexpression increased the level of active β-catenin and enhanced the osteogenic differentiation of hBMSCs. This effect was reversed by the Wnt/β-catenin inhibitor DKK1. Mass spectrometry showed that USP53 interacted with F-box only protein 31 (FBXO31) to promote proteasomal degradation of β-catenin. Inhibition of the osteogenic differentiation of hBMSCs by FBXO31 was partially rescued by USP53 overexpression. Animal studies showed that hBMSCs with USP53 overexpression significantly promoted bone regeneration in mice with calvarial defects. These results suggested that USP53 may be a target for gene therapy for bone regeneration.Subject terms: Cell signalling, Mesenchymal stem cells 相似文献