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91.
92.
Tsai JJ Liu SH Yin SC Yang CN Hsu HS Chen WB Liao EC Lee WJ Pan HC Sheu ML 《PloS one》2011,6(9):e23249
Background
Allergic disease can be characterized as manifestations of an exaggerated inflammatory response to environmental allergens triggers. Mite allergen Der-p2 is one of the major allergens of the house dust mite, which contributes to TLR4 expression and function in B cells in allergic patients. However, the precise mechanisms of Der-p2 on B cells remain obscure.Methodology/Principal Findings
We investigated the effects of Der-p2 on proinflammatory cytokines responses and Toll-like receptor-4 (TLR4)-related signaling in human B cells activation. We demonstrated that Der-p2 activates pro-inflammatory cytokines, TLR4 and its co-receptor MD2. ERK inhibitor PD98059 significantly enhanced TLR4/MD2 expression in Der-p2-treated B cells. Der-p2 markedly activated mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) and decreased p38 phosphorylation in B cells. MKP-1-siRNA downregulated TLR4/MD2 expression in Der-p2-treated B cells. In addition, Der-p2 significantly up-regulated expression of co-stimulatory molecules and increased B cell proliferation. Neutralizing Der-p2 antibody could effectively abrogate the Der-p2-induced B cell proliferation. Der-p2 could also markedly induce NF-κB activation in B cells, which could be counteracted by dexamethasone.Conclusions/Significance
These results strongly suggest that Der-p2 is capable of triggering B cell activation and MKP-1-activated p38/MAPK dephosphorylation-regulated TLR4 induction, which subsequently enhances host immune, defense responses and development of effective allergic disease therapeutics in B cells. 相似文献93.
Chemically sulfated polysaccharide (S-GAP-P) was derived from water-insoluble polysaccharide of Grifola frondosa mycelia. In this research, we investigated the anticancer effects of S-GAP-P and its combination with 5-fluorouracil (5-FU) on human gastric carcinoma SGC-7901 cells. Results showed that S-GAP-P distinctly inhibited SGC-7901 cells growth in a dose-dependent manner and induced cell apoptosis evidenced by characteristic DNA ladder and sub-G0/G1 peak. Furthermore, the combination of S-GAP-P (10–50 μg/ml) with 1 μg/ml 5-FU resulted in a significant inhibition on SGC-7901 cells growth, meaning the beneficial interaction between the two drugs. All these results suggested that S-GAP-P has evident anticancer activity through apoptotic induction and could significantly accelerate the anticancer activity of 5-FU. 相似文献
94.
Oligonucleotide Microarray with RD-PCR Labeling Technique for Detection and Typing of Human Papillomavirus 总被引:4,自引:0,他引:4
Currently, screening for high-risk human papillomavirus (HPV) infection remains an important health concern throughout the
world, because of the close association between certain types of HPV and cervical cancer. In this study, we explore the possibility
of using ∼70mer oligonucleotide microarray for detection and genotyping of HPV. The ∼70mer type-specific oligonucleotide probes
of four different types HPV were designed by using biological software Arraydesigner 2.0, which analyzed the whole genome
sequences of HPV and selected optimal probes. These probes were synthesized and printed onto the surface of glass slides in
order to prepare a low-density microarray. HPV samples were labeled with fluorescence dyes Cy3 using a method of restriction
display polymerase chain reaction (RD-PCR). HPV plasmid DNA was restricted with Sau3A I to produce multiple fragments that
were ligated to adaptors subsequently and used as PCR template. PCR labeling was performed with the fluorescently labeled
universal primer (Cy3-UP) whose sequence is designed according to the adaptor of the RD-PCR approaches. The labeled samples
were hybridized with the oligonucleotide microarray. The scanning results showed that HPV DNA hybridized specifically with
multiple spots correspondingly to show positive signals, whereas no signals were detected of all the negative and blank controls.
These results demonstrated that ∼70mer oligonucleotide microarray can be applied to HPV detection and genotyping. The application
of RD-PCR in the sample labeling can increase significantly the sensitivity of the assay and will be especially useful for
the discriminate diagnosis of multiple pathogens. 相似文献
95.
鳗鲡幼鱼耳石日轮的研究 总被引:15,自引:1,他引:15
本文报道采自辽东半岛沿岸鳗鲡(Anguilla japonica)的白仔鳗和经人工培育的当年幼鳗耳石日轮生长的观察结果。白仔鳗和幼鳗耳石平均直径均与体全长成直线相关。12尾白仔鳗耳石的平均日轮数146.3,据此推测其产卵期为11—12月。观察证实从咸淡水转人到淡水生活的幼鳗耳石的环纹有过渡带存在。 相似文献
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98.
Climate change poses critical challenges for population persistence in natural communities, for agriculture and environmental sustainability, and for food security. In this review, we discuss recent progress in climatic adaptation in plants. We evaluate whether climate change exerts novel selection and disrupts local adaptation, whether gene flow can facilitate adaptive responses to climate change, and whether adaptive phenotypic plasticity could sustain populations in the short term. Furthermore, we discuss how climate change influences species interactions. Through a more in‐depth understanding of these eco‐evolutionary dynamics, we will increase our capacity to predict the adaptive potential of plants under climate change. In addition, we review studies that dissect the genetic basis of plant adaptation to climate change. Finally, we highlight key research gaps, ranging from validating gene function to elucidating molecular mechanisms, expanding research systems from model species to other natural species, testing the fitness consequences of alleles in natural environments, and designing multifactorial studies that more closely reflect the complex and interactive effects of multiple climate change factors. By leveraging interdisciplinary tools (e.g., cutting‐edge omics toolkits, novel ecological strategies, newly developed genome editing technology), researchers can more accurately predict the probability that species can persist through this rapid and intense period of environmental change, as well as cultivate crops to withstand climate change, and conserve biodiversity in natural systems. 相似文献
99.
Deformable energy storage devices are needed to power next‐generation wearable electronics that interface intimately with human skin. Currently, deformable energy storage devices demonstrate poor performance compared to their rigid lithium‐ion counterparts, forcing wearable manufacturers to design their devices around bulky battery compartments. However, technological advances to create deformable batteries at the component and device level have yielded continuous improvement in stretchable batteries over the last five years. In this Essay, the major strategies at the component and device level that have been successfully employed to create stretchable batteries are reviewed. The outstanding challenges facing deformable energy storage are also discussed, namely, energy density, packaging, delamination, device integration, and manufacturing. This Essay will give researchers who are interested in contributing to the development of deformable batteries a cursory understanding of the most successful strategies to date, and provide insights into the most important directions to pursue in the future. 相似文献
100.
Di Zhang Zhenhui Wang Ningning Wang Yang Gao Ying Liu Ying Wu Yan Bai Zhibin Zhang Xiuyun Lin Yuzhu Dong Xiufang Ou Chunming Xu Bao Liu 《PloS one》2014,9(5)