全文获取类型
收费全文 | 2345篇 |
免费 | 144篇 |
国内免费 | 153篇 |
出版年
2024年 | 7篇 |
2023年 | 47篇 |
2022年 | 79篇 |
2021年 | 168篇 |
2020年 | 94篇 |
2019年 | 97篇 |
2018年 | 96篇 |
2017年 | 52篇 |
2016年 | 100篇 |
2015年 | 140篇 |
2014年 | 178篇 |
2013年 | 178篇 |
2012年 | 215篇 |
2011年 | 181篇 |
2010年 | 96篇 |
2009年 | 81篇 |
2008年 | 104篇 |
2007年 | 106篇 |
2006年 | 100篇 |
2005年 | 84篇 |
2004年 | 45篇 |
2003年 | 57篇 |
2002年 | 47篇 |
2001年 | 21篇 |
2000年 | 33篇 |
1999年 | 32篇 |
1998年 | 24篇 |
1997年 | 17篇 |
1996年 | 15篇 |
1995年 | 18篇 |
1994年 | 14篇 |
1993年 | 13篇 |
1992年 | 19篇 |
1991年 | 14篇 |
1990年 | 10篇 |
1989年 | 12篇 |
1988年 | 8篇 |
1987年 | 7篇 |
1986年 | 8篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1974年 | 2篇 |
1971年 | 1篇 |
1966年 | 2篇 |
排序方式: 共有2642条查询结果,搜索用时 31 毫秒
51.
Photosynthesis Research - Formation of photosynthetic complexes leads to a higher demand for Fe–S clusters. We hypothesized that in the facultative phototrophic alpha-proteobacterium... 相似文献
52.
Chen Minmin Nie Gongping Yang Liuyan Zhang Yongchun Cai Youming 《Plant Growth Regulation》2021,95(1):49-64
Plant Growth Regulation - As one of the most popular bulbous plant with high ornamental value, Lilium spp. has been used as cut flower or green plants in urban landscapes and home gardening. We... 相似文献
53.
54.
Sheng Yumeng Cao Manlin Liu Yiwen He Yiqing Zhang Guoliang Du Yan Gao Feng Yang Cuixia 《Molecular and cellular biochemistry》2021,476(9):3383-3391
Molecular and Cellular Biochemistry - Although luminal breast cancer cells are typically highly cohesive epithelial cells and have low invasive ability, many eventually develop metastasis. Until... 相似文献
55.
56.
Xiaoxue Li Changwei Liu Yiwen Zhu Hanyu Rao Min Liu Liming Gui Wenxin Feng Huayuan Tang Jin Xu WeiQiang Gao Li Li 《Cell proliferation》2021,54(6)
ObjectivesCutaneous wound healing is one of the major medical problems worldwide. Epigenetic modifiers have been identified as important players in skin development, homeostasis and wound repair. SET domain–containing 2 (SETD2) is the only known histone H3K36 tri‐methylase; however, its role in skin wound healing remains unclear.Materials and MethodsTo elucidate the biological role of SETD2 in wound healing, conditional gene targeting was used to generate epidermis‐specific Setd2‐deficient mice. Wound‐healing experiments were performed on the backs of mice, and injured skin tissues were collected and analysed by haematoxylin and eosin (H&E) and immunohistochemical staining. In vitro, CCK8 and scratch wound‐healing assays were performed on Setd2‐knockdown and Setd2‐overexpression human immortalized keratinocyte cell line (HaCaT). In addition, RNA‐seq and H3K36me3 ChIP‐seq analyses were performed to identify the dysregulated genes modulated by SETD2. Finally, the results were validated in functional rescue experiments using AKT and mTOR inhibitors (MK2206 and rapamycin).ResultsEpidermis‐specific Setd2‐deficient mice were successfully established, and SETD2 deficiency resulted in accelerated re‐epithelialization during cutaneous wound healing by promoting keratinocyte proliferation and migration. Furthermore, the loss of SETD2 enhanced the scratch closure and proliferation of keratinocytes in vitro. Mechanistically, the deletion of Setd2 resulted in the activation of AKT/mTOR signalling pathway, while the pharmacological inhibition of AKT and mTOR with MK2206 and rapamycin, respectively, delayed wound closure.ConclusionsOur results showed that SETD2 loss promoted cutaneous wound healing via the activation of AKT/mTOR signalling. 相似文献
57.
Sheng Chen Xinheng Zhang Yu Nie Hongxin Li Weiguo Chen Wencheng Lin Feng Chen Qingmei Xie 《中国病毒学》2021,36(2):196-206
African swine fever virus(ASFV), as a member of the large DNA viruses, may regulate autophagy and apoptosis by inhibiting programmed cell death. However, the function of ASFV proteins has not been fully elucidated, especially the role of autophagy in ASFV infection. One of three Pyrroline-5-carboxylate reductases(PYCR), is primarily involved in conversion of glutamate to proline. Previous studies have shown that depletion of PYCR2 was related to the induction of autophagy. In the present study, we found for the first time that ASFV E199 L protein induced a complete autophagy process in Vero and HEK-293 T cells. Through co-immunoprecipitation coupled with mass spectrometry(CoIP-MS)analysis, we firstly identified that E199 L interact with PYCR2 in vitro. Importantly, our work provides evidence that E199 L down-regulated the expression of PYCR2, resulting in autophagy activation. Overall, our results demonstrate that ASFV E199 L protein induces complete autophagy through interaction with PYCR2 and down-regulate the expression level of PYCR2, which provide a valuable reference for the role of autophagy during ASFV infection and contribute to the functional clues of PYCR2. 相似文献
58.
Zhang Xinheng Chen Tong Chen Sheng Nie Yu Xie Zi Feng Keyu Zhang Huanmin Xie Qingmei 《中国病毒学》2021,36(6):1431-1442
Virologica Sinica - Infectious bronchitis (IB) is a highly contagious avian disease caused by infection with infectious bronchitis virus (IBV), which seriously affects the development of the global... 相似文献
59.
Youming Shen Jiyun Nie Lixue Kuang Jianyi Zhang Haifei Li 《Microbial biotechnology》2021,14(2):323-362
The development of DNA sequencing technology has provided an effective method for studying foodborne and phytopathogenic microorganisms on fruits and vegetables (F & V). DNA sequencing has successfully proceeded through three generations, including the tens of operating platforms. These advances have significantly promoted microbial whole-genome sequencing (WGS) and DNA polymorphism research. Based on genomic and regional polymorphisms, genetic markers have been widely obtained. These molecular markers are used as targets for PCR or chip analyses to detect microbes at the genetic level. Furthermore, metagenomic analyses conducted by sequencing the hypervariable regions of ribosomal DNA (rDNA) have revealed comprehensive microbial communities in various studies on F & V. This review highlights the basic principles of three generations of DNA sequencing, and summarizes the WGS studies of and available DNA markers for major bacterial foodborne pathogens and phytopathogenic fungi found on F & V. In addition, rDNA sequencing-based bacterial and fungal metagenomics are summarized under three topics. These findings deepen the understanding of DNA sequencing and its application in studies of foodborne and phytopathogenic microbes and shed light on strategies for the monitoring of F & V microbes and quality control. 相似文献
60.
Enwu Xu Kai Su Yang Zhou Longlong Gong Yiwen Xuan Ming Liao Jiawang Cao Yaqian Li Yujiao Lu Yi Zhao Fengxia Chen 《Journal of cellular and molecular medicine》2021,25(21):10279-10290
Tumour-derived DNA found in the plasma of cancer patients provides the probability to detect somatic mutations from circulating cell-free DNA (cfDNA) in plasma samples. However, clonal hematopoiesis (CH) mutations affect the accuracy of liquid biopsy for cancer diagnosis and treatment. Here, we integrated landscape of CH mutations in 11,725 pan-cancer patients of Chinese and explored effects of CH on liquid biopsies in real-world. We first identified 5933 CHs based on panel sequencing of matched DNA of white blood cell and cfDNA on 301 genes for 5100 patients, in which CH number of patients had positive correlation with their diagnosis age. We observed that canonical genes related to CH, including DNMT3A, TET2, ASXL1, TP53, ATM, CHEK2 and SF3B1, were dominant in the Chinese cohort and 13.29% of CH mutations only appeared in the Chinese cohort compared with the Western cohort. Analysis of CH gene distribution bias indicated that CH tended to appear in genes with functions of tyrosine kinase regulation, PI3K-Akt signalling and TP53 activity, suggesting unfavourable effects of CH mutations in cancer patients. We further confirmed effect of driver genes carried by CH on somatic mutations in liquid biopsy of cancer patients. Forty-eight actionable somatic mutations in 17 driver genes were considered CH genes in 92 patients (1.80%) of the Chinese cohort, implying potential impacts of CH on clinical decision-making. Taken together, this study exhibits strong evidence that gene mutations from CH interfere accuracy of liquid biopsies using cfDNA in cancer diagnosis and treatment in real-world. 相似文献