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排序方式: 共有430条查询结果,搜索用时 15 毫秒
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You Wang Yushu Wang Yuqi Wu Yang Suo Huaqing Guo Yineng Yu Ruonan Yin Rui Xi Jiajie Wu Nan Hua Yuehan Zhang Shaobo Zhang Zhenming Jin Lin He Gang Ma 《Engineering in Life Science》2023,23(2):e2200034
Clustering enzymes in the same metabolic pathway is a natural strategy to enhance productivity. Synthetic protein, RNA and DNA scaffolds have been designed to artificially cluster multiple enzymes in the cell, which require complex construction processes and possess limited slots for target enzymes. We utilized the Escherichia coli inner cell membrane as a native scaffold to cluster four fatty acid synthases (FAS) and achieved to improve the efficiency of fatty acid synthesis in vivo. The construction strategy is as simple as fusing target enzymes to the N-terminus or C-terminus of the membrane anchor protein (Lgt), and the number of anchored enzymes is not restricted. This novel device not only presents a similar efficiency in clustering multiple enzymes to that of other artificial scaffolds but also promotes the product secretion, driving the entire metabolic flux forward and further increasing the gross yield compared with that in a cytoplasmic scaffold system. 相似文献
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Wang Liyuan Li Huawei Sun Peng Suo Yujing Han Weijuan Diao Songfeng Mai Yini Li Fangdong Fu Jianmin 《Journal of Plant Growth Regulation》2021,40(3):1121-1138
Journal of Plant Growth Regulation - The sex types of persimmon (Diospyros kaki Thunb.) flowers are occasionally alterable. The feminizing agents such as ethrel, 5-azacytidine, a mixture of... 相似文献
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Han-zhou Qi Yi-ling Ye Yuan Suo Hong Qu Hai-yan Zhang Kai-bo Yang Zhi-ping Fan Fen Huang Li Xuan Yan-qiu Chen Hua Jin Qi-fa Liu 《Cell death & disease》2021,12(4)
Chronic graft-versus-host disease (cGVHD) is the main cause of non-relapse mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Mesenchymal stem cells (MSCs) in bone marrow (BM) remain unclear in the pathophysiology of cGVHD. In this study, we analyzed BM-MSCs from 66 patients after allo-HSCT, including 33 with active cGVHD and 33 without cGVHD. BM-MSCs showed similar morphology, frequency, phenotype, and proliferation in patients with or without cGVHD. MSCs from the active cGVHD group showed a decreased apoptosis rate (P < 0.01). Osteogenic capacity was increased while adipogenic capacity was decreased in the active cGVHD MSCs compared with no-cGVHD MSCs. The expressions of osteogenic gene RUNX2 and COL1A1 were higher (P < 0.001) while adipogenic gene PPAR-γ and FABP4 were lower (P < 0.001) in the active cGVHD MSCs than no-cGVHD MSCs. These changes were associated with the severity of cGVHD (P < 0.0001; r = 0.534, r = 0.476, r = −0.796, and r = −0.747, respectively in RUNX2, COL1A1, PPAR-γ, and FABP4). The expression of Wnt/β-catenin pathway ligand Wnt3a was increased in cGVHD-MSCs. The dysfunction of cGVHD-MSCs could be reversed by Dickkopf related protein 1(DKK1) to inhibit the binding of Wnt3a. In summary, the differentiation of BM-MSCs was abnormal in active cGVHD, and its underlying mechanism is the upregulated of Wnt3a through Wnt/β-catenin signaling pathway of MSCs.Subject terms: Cell signalling, Mesenchymal stem cells 相似文献