全文获取类型
收费全文 | 197651篇 |
免费 | 8715篇 |
国内免费 | 7403篇 |
出版年
2024年 | 216篇 |
2023年 | 1453篇 |
2022年 | 3242篇 |
2021年 | 5481篇 |
2020年 | 3582篇 |
2019年 | 4379篇 |
2018年 | 15025篇 |
2017年 | 12890篇 |
2016年 | 11364篇 |
2015年 | 7214篇 |
2014年 | 8129篇 |
2013年 | 8582篇 |
2012年 | 13817篇 |
2011年 | 20819篇 |
2010年 | 16685篇 |
2009年 | 12495篇 |
2008年 | 14919篇 |
2007年 | 15786篇 |
2006年 | 4611篇 |
2005年 | 3816篇 |
2004年 | 3861篇 |
2003年 | 3524篇 |
2002年 | 2965篇 |
2001年 | 2252篇 |
2000年 | 2017篇 |
1999年 | 1865篇 |
1998年 | 1024篇 |
1997年 | 1158篇 |
1996年 | 1027篇 |
1995年 | 919篇 |
1994年 | 948篇 |
1993年 | 695篇 |
1992年 | 1012篇 |
1991年 | 875篇 |
1990年 | 620篇 |
1989年 | 566篇 |
1988年 | 502篇 |
1987年 | 424篇 |
1986年 | 388篇 |
1985年 | 390篇 |
1984年 | 218篇 |
1983年 | 214篇 |
1982年 | 139篇 |
1981年 | 114篇 |
1980年 | 108篇 |
1979年 | 115篇 |
1978年 | 78篇 |
1974年 | 74篇 |
1972年 | 308篇 |
1971年 | 316篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
791.
792.
J M Wang Z G Chen G J Cianciolo R Snyderman F Breviario E Dejana A Mantovani 《Journal of immunology (Baltimore, Md. : 1950)》1989,142(6):2012-2017
The effects of IL-1 on vascular endothelium result in a complex set of alterations which are potentially disruptive of vessel wall and underlying tissue integrity. The present study was aimed at investigating possible regulation of such potentially destructive responses elicited by IL-1 on endothelial cells. Culture supernatants of IL-1-treated human umbilical vein endothelial cells (HEC) were depleted of retroviral p15E-related Ag with immobilized anti-p15E mAb. The monocyte chemotactic and polarizing activity of supernatants of IL-1-treated HEC (presumably related to colony-stimulating factors being released by HEC) was markedly augmented by absorption on immobilized anti-p15E antibodies. Irrelevant IgG had no effect and anti-p15E antibodies did not affect the chemotactic activity of supernatants from unstimulated HEC. The material eluted from Sepharose-bound anti-p15E antibodies was devoid of chemotactic and polarizing activity and suppressed the polarization and migration of monocytes in response to chemoattractants. The alpha and beta molecular species of IL-1 were equally effective in inducing the production of p15E-related inhibitor. The production of a p15E-related inhibitor of chemotaxis induced by IL-1 in HEC may represent a negative signal in the regulation of the potentially destructive responses to pro-inflammatory cytokines. 相似文献
793.
Protein kinase C (PKC) activity and translocation in response to the phorbol ester, phorbol 12-myristate, 13-acetate (PMA), serotonin (5-HT) and thrombin was assessed in human platelets. Stimulation with PMA and 5-HT for 10 minutes or thrombin for 1 minute elicited platelet PKC translocation from cytosol to membrane. The catecholamines, norepinephrine or epinephrine at 10 microM concentrations did not induce redistribution of platelet PKC. Serotonin (0.5-100 microM) and the specific 5-HT2 receptor agonist, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) (10-100 microM) but not the 5-HT1A or 5-HT1B agonists, (+/-) 8-hydroxy-dipropylamino-tetralin (8-OH-DPAT) or 5-methoxy-3-3-(1,2,3,6-tetrahydro-4-pyridin) 1H-indole succinate (RU 24969) induced dose-dependent PKC translocations. Serotonin-evoked PKC translocation was blocked by selective 5-HT2 receptor antagonists, ketanserin and spiroperidol. These results suggest that, in human platelets, PMA, thrombin and 5-HT can elicit PKC translocation from cytosol to membrane. Serotonin-induced PKC translocation in platelets is mediated via 5-HT2 receptors. 相似文献
794.
X. -R. Wang A. E. Szmidt 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1990,80(5):635-640
Summary Restriction fragment analysis and heterologous hybridization of chloroplast (cp) DNA was used to develop species-specific markers for P. tabulaeformis, P. yunnanensis and P. massoniana. Fragment patterns created by the BclI and DraI restriction enzymes and hybridization patterns to the psbC and psbD probes were distinctive among the three species. No intraspecific variation was detected with respect to any of the cpDNA markers developed in this study. The cpDNA markers obtained were subsequently used to examine the parentage of P. densata, a putative Tertiary hybrid between P. tabulaeformis and P. yunnanensis. The analysis demonstrated for the first time that P. densata populations accommodate chloroplast genomes of P. tabulaeformis and P. yunnanensis, which strongly supports earlier suggestions of the hybrid origin of this species. It appears that P. densata represents a stabilized natural hybrid that has become adapted to high mountain environments where neither of the parental species can normally grow. 相似文献
795.
R C Eisensmith Y Okano M Dasovich T Wang F Güttler H Lou P Guldberg U Lichter-Konecki D S Konecki E Svensson 《American journal of human genetics》1992,51(6):1355-1365
Phenylketonuria (PKU), a disorder of amino acid metabolism prevalent among Caucasians and other ethnic groups, is caused primarily by a deficiency of the hepatic enzyme phenylalanine hydroxylase (PAH). PKU is a highly heterogeneous disorder, with more than 60 molecular lesions identified in the PAH gene. The haplotype associations, relative frequencies, and distributions of five prevalent PAH mutations (R158Q, R261Q, IVS10nt546, R408W, and IVS12n1) were established in a comprehensive European sample population and subsequently were examined to determine the potential roles of several genetic mechanisms in explaining the present distribution of the major PKU alleles. Each of these five mutations was strongly associated with only one of the more than 70 chromosomal haplotypes defined by eight RFLPs in or near the PAH gene. These findings suggest that each of these mutations arose through a single founding event that occurred within time periods ranging from several hundred to several thousand years ago. From the significant differences observed in the relative frequencies and distributions of these five alleles throughout Europe, four of these putative founding events could be localized to specific ethnic subgroups. Together, these data suggest that there were multiple, geographically and ethnically distinct origins for PKU within the European population. 相似文献
796.
钐在小鼠肝脏细胞中的动态观察 总被引:8,自引:0,他引:8
It is generally considered that the rare earth compounds are plasma membrane-impermeable, thus affecting the cells only on their surface. Recently, we found that after repeated injections to mice of large dose of samarium trichloride, a soluble compound of rare earth, samarium aggregates appeared in Kupffer cells and hepatocytes of liver. In this study, we aimed at observing the route by which samarium enters the liver cells and the process of the formation of samarium aggregates. Samarium trichloride was given to Swiss mice at one dose of 70 mg/kg intravenously. Thereafter, at different intervals from 15 min to 48 h after the injection, the samarium in liver was traced dynamically by electron microscopy and X ray microanalysis. From 15 min to 2 h both Kupffer cells and hepatocytes endocytosed samarium-containing particles and formed phagosomes, in which the ingested particles were progressively concentrated. Besides, the small phagosomes fused with each other. Phagocytosis was especially active in Kupffer cells. During the 4 h to 24 h many Kupffer cells were degenerated and broken. In hepatocytes the phagosomes gathered mostly around the bile canaliculi. Groups of highly electron-dense particles were found in the lumen of bile canaliculi, implying the excretion of samarium by bile. At the 48 h, the samarium-containing phagosomies were found still in both kinds of cells in the liver. 相似文献
797.
Nieper(1969)依据澳大利亚 Queenslaad 西部 Nora 组(下奥陶统)的标本以 Trigonodus triangularius Nieper 1969 为模式种,建立了牙形刺新属 Trigonodus. Cooper(1981)将 Nieper 的形式属 Trigonodus 修正为器官属,包括 PM Sa Sb Sc Sd 分子,并将此属的模式种改为 Trigonodus larapintinensis (Crespin, 1943). Cooper 的观点被很多牙形刺专家接受, Trigonodus 属名也被广泛引用在牙形刺文献中 (Stauge, 1984;安泰庠、郑昭昌,1990), 相似文献
798.
799.
A stable xylan suspension was prepared and characterized. Hydrolysis of the particles converts them into soluble fragments, thereby lowering the turbidity of the suspension. The small volume of the assay mixture, the short incubation time required, and the simplicity of the procedure permit the rapid analysis of many samples. Furthermore, the procedure can be used to assay xylanase activities in the presence of other reducing materials and is also useful for monitoring low-level xylanase activities. 相似文献
800.
Transfer of IncP Plasmids to Extremely Acidophilic Thiobacillus thiooxidans 总被引:9,自引:1,他引:8
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10-5 to 10-7 per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidans at a frequency of 1.0 × 10-3 per recipient. 相似文献