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71.
72.
Ning Xu Yijie Dong Xinxin Cheng Qilin Yu Kefan Qian Jiwei Mao Chang Jia Xiaohui Ding Bing Zhang Yulu Chen Biao Zhang Laijun Xing Mingchun Li 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》2014
Iron bioavailability is crucial for mitochondrial metabolism and biosynthesis. Dysregulation of cellular iron homeostasis affects multiple aspects of mitochondrial physiology and cellular processes. However, the intracellular iron trafficking pathway in Candida albicans remains unclear. In this study, we characterized the Mrs4–Ccc1–Smf3 pathway, and demonstrated its important role in maintaining cellular iron levels. Double deletion of vacuolar iron exporter SMF3 and mitochondrial iron transporter MRS4 further elevated cellular iron levels in comparison with the single MRS4 deletion. However, deletion of vacuolar iron importer CCC1 in the mrs4?/? mutant restored cellular iron homeostasis to normal wild-type levels, and also normalized most of the defective phenotypes in response to various environmental stresses. Our results also suggested that both Mrs4 and Ccc1 contributed to the maintenance of mitochondrial function. The mrs4?/? and mrs4?/?smf3?/? mutants exhibited an obvious decrease in aconitase activities and mitochondrial membrane potential, whereas deletion of CCC1 in the mrs4?/? mutant effectively rescued these defects. Furthermore, we also found that the Mrs4–Ccc1–Smf3 pathway was indispensable for cell-wall stability, antifungal drug tolerance, filamentous growth and virulence, supporting the novel viewpoint that mitochondria might be the promising target for better antifungal therapies. Interestingly, the addition of exogenous iron failed to rescue the defects on non-fermentable carbon sources or hyphae-inducing medium, indicating that the defects in mitochondrial respiration and filamentous development might result from the disturbance of cellular iron homeostasis rather than environmental iron deprivation. Taken together, our results propose the Mrs4–Ccc1–Smf3 pathway as a potentially attractive target for antifungal drug development. 相似文献
73.
Jiwen Wang Jinyu Wei Xiaoling Xu Wenting Pan Yunxia Ge Changchun Zhou Chao Liu Jia Gao Ming Yang Weimin Mao 《PloS one》2014,9(4)
China was one of the countries with highest esophageal squamous cell carcinoma (ESCC) incidence and mortality worldwide. Alcohol drinking has been identified as a major environmental risk-factor related to ESCC. The alcohol dehydrogenase (ADH) family are major enzymes involved in the alcohol-metabolizing pathways, including alcohol dehydrogenase 1B (ADH1B) and ADH1C. Interestingly, ADH1B and ADH1C genes locate tandemly with ADH7 in a genomic segment as a gene cluster, and are all polymorphic. Several ESCC susceptibility single nucleotide polymorphisms (SNPs) of the ADH1B-ADH1C-ADH7 cluster have been identified previously through a genome-wide association study (GWAS). In the study, we examined the association between five ADH1B-ADH1C-ADH7 cluster SNPs (rs1042026, rs17033, rs1614972, rs1789903 and rs17028973) and risk of developing ESCC. Genotypes were determined in two independent case-control sets from two regions of China. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated by logistic regression. Our data demonstrated that these ADH1B-ADH1C-ADH7 cluster SNPs confer susceptibility to ESCC in these two case-control sets, which were consistent to results of the previous GWAS. 相似文献
74.
A single Rad52-related protein is evident by blast analysis of the Ustilago maydis genome database. Mutants created by disruption of the structural gene exhibited few discernible defects in resistance to UV, ionizing radiation, chemical alkylating or cross-linking agents. No deficiency was noted in spontaneous mutator activity, allelic recombination or meiosis. GFP-Rad51 foci were formed in rad52 cells following DNA damage, but were initially less intense than normal suggesting a possible role for Rad52 in formation of the Rad51 nucleoprotein filament. A search for interacting genes that confer a synthetic fitness phenotype with rad52 after DNA damage by UV irradiation identified the genes for Mph1, Ercc1 and the Rad51 paralogue Rec2. Testing known mutants in recombinational repair revealed an additional interaction with the BRCA2 orthologue Brh2. Suppression of the rec2 mutant's UV sensitivity by overexpressing Brh2 was found to be dependent on Rad52. The results suggest that Rad52 serves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament. 相似文献
75.
通过生物素与亲和素-酶复合物系统或地高辛与抗地高辛-酶复合物系统可把酶间接标记到探针上.Renz等通过不同的化学方法直接把酶标记到探针上[1~3].耐热性碱性磷酸酯酶FD-TAP(thermostablealkalinephosphatase)具有耐... 相似文献
76.
miR319在植物器官发育中的调控作用 总被引:1,自引:0,他引:1
microRNAs(miRNAs)是一类内源性的、21~25个碱基长度的小分子非编码RNA,它通过指导剪切或者抑制翻译等方式调节植物基因的表达,参与调控植物生长发育各个方面。大量研究表明,miR319通过靶向TCPs转录因子控制植物叶、花等器官的生长命运,并参与调控部分激素生物合成和信号传导通路,在植物发育过程中发挥重要生物学功能。文章综述了miR319在植物叶形态建成、生长发育以及叶衰老和花器官发育等过程中的重要调控作用。 相似文献
77.
A bottleneck in recent gene synthesis technologies is the high cost of oligonucleotide synthesis and post-synthesis sequencing.
In this article, a simple and rapid method for low-cost gene synthesis technology was developed based on DNAWorks program
and an improved single-step overlap extension PCR (OE-PCR). This method enables any DNA sequence to be synthesized with few
errors, then any mutated sites could be corrected by site-specific mutagenesis technology or PCR amplification-assembly method,
which can amplify different DNA fragments of target gene followed by assembly into an entire gene through their overlapped
region. Eventually, full-length DNA sequence without error was obtained via this novel method. Our method is simple, rapid
and low-cost, and also easily amenable to automation based on a DNAWorks design program and defined set of OE-PCR reaction
conditions suitable for different genes. Using this method, several genes including Manganese peroxidase gene (Mnp) of Phanerochaete chrysosporium (P. chrysosporium), Laccase gene (Lac) of Trametes versicolor (T. versicolor) and Cip1 peroxidase gene (cip 1) of Coprinus cinereus (C. cinereus) with sizes ranging from 1.0 kb to 1.5 kb have been synthesized successfully.
Bingxue Dong and Runqian Mao contributed equally to this work. 相似文献
78.
光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响 总被引:1,自引:0,他引:1
白光、蓝光、红光和远红光等不同光质照射及不同的白光强度对水母雪莲愈伤组织生长、苯丙氨酸氨基裂解酶(PAL) 活性及黄酮合成有不同的影响。红光明显促进愈伤组织的生长,但强烈抑制PAL活性和黄酮的合成。蓝光对愈伤组织生长无明显影响,但显著提高PAL 活性和黄酮的合成。白光的作用介于红光和蓝光之间。远红光的作用与蓝光相似,但作用比较弱。每天16 h 蓝光加8 h 白光和8 h 蓝光加16 h 白光的组合产生最高的黄酮含量和黄酮生产率。 相似文献
79.
80.
Hongyan Chen Bing Sun Yingjie Zhao Xiao Song Weiwei Fan Keke Zhou Liangfu Zhou Ying Mao Daru Lu 《PloS one》2012,7(12)