人肾液泡型H~＋_－ATPase 58kD亚基基因的表达

在大肠杆菌中表达了人肾液泡型Ｈ￣＋－ＡＴＰａｓｅ５８ｋＤ亚基的基因,利用聚合酶链式反应（ＰＣＲ）得到了５８ｋＤ亚基的编码片段．直接将ＰＣＲ产物连接到ＰＥＴ载体上表达．ＳＤＳ聚丙烯酰胺凝胶电泳和蛋白质印迹分析表明５８ｋＤ亚基的基因得到高效表达．表达产物可达细菌细胞质蛋白的５０％．     

玉米胚性愈伤组织的长期继代及其染色体分析

对５种基因型幼胚诱导的愈伤组织继代培养表明,玉米胚性愈伤组织的长期继代受基因型,培养基成分,激素,培养条件的影响。适时继代,逐代筛选对胚性保持起重要作用。适当降低培养温度（１２±２℃）有利于愈伤组织的保存和胚性保持,可以减少愈伤组织长期继代所需的物质和工作量。长期继代培养的胚性愈伤组织,胚状体发生能力和植株再生率无显著变化,但正常苗的再生频率显著下降。观察愈伤组织细胞染色体发现：（１）基因型对不同倍性细胞的比例有明显影响。（２）随着继代时间的延长,二倍体细胞下降,四倍体和非二倍体细胞增多。（３）愈伤组织中出现多种染色体结构变异,这些结构变异有可能导致非整倍体细胞的形成。     

甘肃稀有濒危植物的地理分布和区系特征

分析了甘肃稀有濒危植物５１个种的地理分布和４８个属的分布区类型及其区系特征。     

细胞电穿孔动态过程的荧光测量

利用改进后的Ｔｈ／ＤＰＡ荧光方法及探针ＥＢ对人血影及大鼠骨髓细胞电穿孔的动态过程及其与电脉冲参数的关系进行了系统的研究．测量结果表明,在临界点以上电场作用下,血影电穿孔在电击后０．２—０．３ｓ时达最大,在约０．８ｓ时愈合;而大鼠骨髓细胞电穿孔在电击后０．４—０．９ｓ达到最大,３－５ｓ左右愈合;电穿孔大小及扩大、愈合速率与电脉冲参数有关。１０－４０ｍｍｏｌ／Ｌ乙醇和５－２０ｍｍｏｌ／Ｌ成二醛抑制血影对Ｔｂ３＋离子的电通透,相同浓度的成二醛作用强于乙醇。这些结果将为电穿孔技术的合理应用提供参考。     

Fertile plants regenerated from suspension culture-derived protoplasts of an indica type rice (Oryza sativa L.)

Calluses were induced from immature embryos of an indica type rice and finely dispersed cell suspension cultures were initiated from the callus using modified AA medium (S₁ medium). The suspension cultures were maintained alternatively (1–2 passages in each medium) in S₁ medium and S₂ medium, the latter containing KNO₃, NH₄NO₃, proline and glutamine as nitrogen source. Protoplasts of high quality were isolated form suspension cells cultured in S₂ medium supplemented with ABA. Embedding the protoplasts in agarose blocks containing NH₄NO₃-free modified KM8P(PM₁) medium and immersing the blocks in NH₄NO₃-containing modified KM8P(PM₃) medium were most effective for obtaining protoplast division and callus formation. The protoplast-derived calluses were precultured in potato extract-aand/or ABA-containing N₆(D₁, D₂ or D₃) media and many embryo-like structures were formed. These structures developed into plantlets after being transferred to N₆ differentiation (D₄) medium. The regenerated plantlets grew into mature plants and beard seeds normally.Abbreviations AA medium amino acids based medium - ABA abscisic acid - BA benzyladenine - 2,4-D 2,4-dichlorophenoxyacetic acid - DF division frequency - IAA indoleacetic acid - KIN kinetin - NAA naphthaleneacetic acid - PE planting efficiency     

Transport kinetics of maltotriose in strains ofSaccharomyces

Summary Maltotriose transport was studied in two brewer's yeast strains, an ale strain 3001 and a lager strain 3021, using laboratory-synthesized¹⁴C-maltotriose. The maltotriose transport systems preferred a lower pH (pH 4.3) to a higher pH (pH 6.6). Two maltotriose transport affinity systems have been indentified. The high affinity system hasK _m values of 1.3 mM for strain 3021 and 1.4 mM for strain 3001. The low affinity competitively inhibited by maltose and glucose withK _i values of 58 mM and 177 mM. respectively, for strain 3021, and 55 mM and 147 mM, respectively, for strain 3001. Cells grown in maltotriose and maltose had higher maltotriose and maltose transport rates, and cells grown in glucose had lower maltortriose and maltose transport rates. Early-logarithmic phase cells transported glucose faster than either maltose or maltotriose. Cells harvested later in the growth phase had increased maltotriose and maltose transport activity. Neither strain exhibited significant differences with respect to maltose and maltotriose transport activity.     

海南省粉褶蕈属的分类研究(Ⅱ)

本文报道了海南省粉褶蕈属[Entoloma(Fr.)Kumm.]的31个种,其中17个为新记录种。并附分种检索表,表中含6个新种。 所有标本保藏于广东省微生物研究所真菌标本室。     

黄桃罐头产品质量Fuzzy综合鉴评

本文根据FuzzZ数学理论,运用多级模型的综合评判法,对国内20种黄桃罐头产品质量进行了优劣鉴评。并将其划分为一、二、三、四4个等级。从而为罐头产品质量鉴评,准确地汰劣遴优提供新方法。     

零下低温对杂交杨树皮层膜脂组成的影响

以不耐寒的美洲黑杨（Ｐｏｐｕｌｕｓｄｅｌｔｏｉｄｅｓｃｖ．“Ｌｕｘ”Ｉ－６９／５５,父本）和耐寒性较强的欧美杨（Ｐ．ｅｕｒａｍｅｒｉｃａｎａｃｌｃｖ．Ｉ－４５／５１,母本）的４个杂交Ｆ＿１代无性系（９５杨、５５９杨、６００杨和１３８１杨）为材料,分析了零下低温寒潮前后枝条皮层的脂质组成。结果表明,寒潮影响下,皮层中磷脂含量增加而组成基本不变,膜脂脂肪酸组成的变化规律是：寒潮前脂肪酸不饱和指数（ＩＵＦＡ）值大的无性系,寒潮前后的ＩＵＦＡ值变化量小;寒潮前ＩＵＦＡ值较小的无性系,寒潮前后ＩＵＦＡ值变化量较大。本文借用力学概念,提出相对抗性概念,给出杨树无性系的相对抗性序列。序列表明Ｆ＿１代无性系的耐寒性已较不耐寒的父本提高,这与田间观察基本一致。     

精子作载体的转基因鱼研究

本文报道了以精子为载体将美洲拟蝶抗冻蛋白基因导入罗非鱼卵,构建转基因鱼的方法,此法简单易行。斑点杂文和ＳｏｕｔｈｅｒｎＢｌｏｔ杂交结果表明,外源基因的整合率为１８．１％,与其它方法构建转基因鱼的外源基因整合率相近。