全文获取类型
收费全文 | 24443篇 |
免费 | 2229篇 |
国内免费 | 3134篇 |
专业分类
29806篇 |
出版年
2024年 | 90篇 |
2023年 | 405篇 |
2022年 | 836篇 |
2021年 | 1311篇 |
2020年 | 918篇 |
2019年 | 1196篇 |
2018年 | 1061篇 |
2017年 | 747篇 |
2016年 | 1062篇 |
2015年 | 1654篇 |
2014年 | 1899篇 |
2013年 | 2022篇 |
2012年 | 2418篇 |
2011年 | 2132篇 |
2010年 | 1400篇 |
2009年 | 1324篇 |
2008年 | 1449篇 |
2007年 | 1270篇 |
2006年 | 1134篇 |
2005年 | 900篇 |
2004年 | 800篇 |
2003年 | 722篇 |
2002年 | 585篇 |
2001年 | 350篇 |
2000年 | 309篇 |
1999年 | 272篇 |
1998年 | 247篇 |
1997年 | 177篇 |
1996年 | 164篇 |
1995年 | 129篇 |
1994年 | 142篇 |
1993年 | 84篇 |
1992年 | 120篇 |
1991年 | 71篇 |
1990年 | 53篇 |
1989年 | 53篇 |
1988年 | 30篇 |
1987年 | 49篇 |
1986年 | 35篇 |
1985年 | 35篇 |
1984年 | 19篇 |
1983年 | 18篇 |
1982年 | 16篇 |
1981年 | 13篇 |
1980年 | 6篇 |
1979年 | 14篇 |
1976年 | 7篇 |
1973年 | 8篇 |
1972年 | 6篇 |
1968年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
本文通过研究提出了一种由大肠杆菌细胞中提取重组猪生长激素的方法。提取回收率为52.4%,纯度为94.1%,具有生物学活性。 相似文献
62.
T cell-derived IL-4 has many effects on murine B cells, including the up-regulation of class II antigens and the induction of isotype switching. The development of memory B cells and the decreased expression of J11d antigens on these cells are influenced by T cells. In this report, we determined whether the decreased expression of J11d can also be mediated by T cell-derived lymphokines and, in particular, IL-4. We found that IL-4 can down-regulate the expression of J11d on both large and small B cells, that this effect becomes significant after 48 hr of culture and occurs at doses of IL-4 that are similar to those required to up-regulate murine class II MHC antigens encoded by I-region alpha genes (IA). Anti-IL-4 antibody completely blocks this effect but IFN-gamma does not. Other lymphokines (IL-1, IL-2, IL-3, IL-5, IL-6) neither induce a decrease in J11d nor alter the ability of IL-4 to down-regulate J11d expression. The decrease in J11d expression on B cells is not due to a preferential survival of cells expressing lower levels of J11d, although IL-4 has a more pronounced effect on these B cells. Finally, the down-regulation of J11d and up-regulation of IA by IL-4 occurs on all inducible B cells. 相似文献
63.
为了解四川省自贡地区3~5岁幼儿乙型肝炎病毒(HBV)感染情况,并探索与感染有关的因素,1985年调查了1167名幼儿,其HBV总感染率为41.13%,HBsAg阳性率12.68%。幼儿的HBV感染与母亲HBsAg阳性密切相关。共检查母亲409例,38例HBsAg阳性,其幼儿HBsAg阳性率为50%(19/38),HBsAg阴性的母亲371例,其幼儿HBsAg阳性率9.97%(37/371),来自HBsAg阳性母亲的阳性子女占33.3%(19/56)。1986年随访HBV易感幼儿448例,HBV年感染率为12.95%(58/448),HBsAg年阳转率3.79%。HBV年感染率与原幼儿班级HBsAg阳性率的高低有关。 相似文献
64.
据我们在北京和内蒙古等地多年研究表明,黄芪的植食性种子小蜂包括广肩小蜂科Bruchophagus属5种和金小蜂科Habrocytus属1种,为多种混合群体。我们通过大量标本的形态比较以及交配行为的观察,结合其生物学特性研究以及应用扫描电镜对其并胸腹节花纹的超微结构观察,鉴定出黄芪种子里有5种广肩种子小蜂,即黄芪种子小蜂Bruchophagus huonchi Liao et Fan及本文的4新种。 相似文献
65.
66.
Wild-type p53 restores cell cycle control and inhibits gene amplification in cells with mutant p53 alleles. 总被引:113,自引:0,他引:113
Loss of cell cycle control and acquisition of chromosomal rearrangements such as gene amplification often occur during tumor progression, suggesting that they may be correlated. We show here that the wild-type p53 allele is lost when fibroblasts from patients with the Li-Fraumeni syndrome (LFS) are passaged in vitro. Normal and LFS cells containing wild-type p53 arrested in G1 when challenged with the uridine biosynthesis inhibitor PALA and did not undergo PALA-selected gene amplification. The converse occurred in cells lacking wild-type p53 expression. Expression of wild-type p53 in transformants of immortal and tumor cells containing mutant p53 alleles restored G1 control and reduced the frequency of gene amplification to undetectable levels. These studies reveal that p53 contributes to a metabolically regulated G1 check-point, and they provide a model for understanding how abnormal cell cycle progression leads to the genetic rearrangements involved in tumor progression. 相似文献
67.
68.
69.
Purification and structural properties of gelsolin, a Ca2+-activated regulatory protein of macrophages 总被引:30,自引:0,他引:30
We describe the purification procedure and some of the physiochemical properties of gelsolin, a major Ca2+-dependent regulatory protein of actin gel-sol transformation in rabbit lung macrophages. Gelsolin accounts for the majority of Ca2+ control of actin gelation in macrophage extracts. It is a single polypeptide chain with an average molecular weight of 91,000 a Stokes radius of 44 A, a sedimentation coefficient (s20(0),w) of 4.9 S, an isoelectric point of 6.1, and a frictional ratio of 1.43. Gelsolin binds 2 mol of Ca2+ with high affinity (Ka 1.09 X 10(6) M-1) in the presence of 0.1 M KCl and 2 mM MgCl2. 相似文献
70.
Repeated Biogel P6 chromatography of the urine from a patient with fucosidosis yielded several fractions containing fucosyloligosaccharides and glycopeptides. Two of these were shown by 1H nuclear magnetic resonance (1H-n.m.r.) spectroscopy and permethylation analysis to have the following structures respectively: (I) αfuc (1→3) [βgal (1→4)] βglcNAc (1→2) αman () βman (1→4) glcNAc and (II) αfuc (1→3) [βgal (1→4)] βglcNAc (1→2) αman () βman (1→4) βglcNAc (1→4) [αfuc ()] βglcNAc-Asn. 相似文献