全文获取类型
收费全文 | 31644篇 |
免费 | 3527篇 |
国内免费 | 2384篇 |
出版年
2023年 | 292篇 |
2022年 | 591篇 |
2021年 | 1041篇 |
2020年 | 735篇 |
2019年 | 910篇 |
2018年 | 854篇 |
2017年 | 680篇 |
2016年 | 920篇 |
2015年 | 1491篇 |
2014年 | 1725篇 |
2013年 | 1914篇 |
2012年 | 2314篇 |
2011年 | 2158篇 |
2010年 | 1481篇 |
2009年 | 1339篇 |
2008年 | 1623篇 |
2007年 | 1542篇 |
2006年 | 1386篇 |
2005年 | 1213篇 |
2004年 | 1108篇 |
2003年 | 1045篇 |
2002年 | 927篇 |
2001年 | 694篇 |
2000年 | 693篇 |
1999年 | 600篇 |
1998年 | 395篇 |
1997年 | 293篇 |
1996年 | 270篇 |
1995年 | 233篇 |
1994年 | 242篇 |
1993年 | 219篇 |
1992年 | 414篇 |
1991年 | 313篇 |
1990年 | 345篇 |
1989年 | 302篇 |
1988年 | 312篇 |
1987年 | 342篇 |
1986年 | 260篇 |
1985年 | 289篇 |
1984年 | 231篇 |
1983年 | 243篇 |
1982年 | 208篇 |
1981年 | 187篇 |
1979年 | 244篇 |
1978年 | 209篇 |
1977年 | 181篇 |
1976年 | 185篇 |
1975年 | 170篇 |
1974年 | 171篇 |
1973年 | 185篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
42.
43.
Marjorie Jones Roy W. Keenan 《Biochimica et Biophysica Acta (BBA)/General Subjects》1981,678(3):403-407
A procedure was developed for the detection of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in myelin. This assay was sufficiently sensitive to detect the low levels of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in human erythrocytes. The 2′,3′-cyclic nucleotide 3′-phosphohydrolase of human erythrocytes was determined to be exclusively associated with the inner (cytosolic) side of the membrane. Leaky ghostsand resealed ghosts were assayed for 2′,3′-cyclic nucleotide 3′-phosphohydrolase, (Ca2+/Mg2+-ATPase, and acetylcholinesterase activity, and the 2′,3′-cyclic nucleotide 3′-phosphohydrolase profile is the same as that of the (Ca2+/Mg2+)-ATPase, an established inner membrane maker. 相似文献
44.
G Murray Jones 《BMJ (Clinical research ed.)》1981,283(6303):1406-1407
45.
Background
Eosinophilia plays the major role in the pathogenesis of asthma and correlates with the up‐regulation of eotaxin, which, together with interleukin (IL)‐5, is important for differentiation, chemo‐attraction, degranulation, and survival of eosinophils in local tissue. In a previous study, we found that administration of lentivirus‐delivered short hairpin RNA (shRNA) to suppress the expression of IL‐5 inhibited airway inflammation. The present study aimed to investigate the role of eotaxin shRNA and the synergistic effect of eotaxin and IL‐5 shRNAs on airway inflammation in an ovalbumin (OVA)‐induced murine model of asthma.Methods
Lentivirus‐delivered shRNAs were used to suppress the expression of eotaxin and/or IL‐5 in local tissue in an OVA‐induced murine asthma model.Results
Intra‐tracheal administration of lentivirus containing eotaxin shRNA expressing cassette (eoSEC3.3) efficiently moderated the characteristics of asthma, including airway hyper‐responsiveness, cellular infiltration of lung tissues, and eotaxin and IL‐5 levels in bronchio‐alveolar lavage fluid. Administration of lentiviruses expressing IL‐5 or eotaxin shRNAs (IL5SEC4 + eoSEC3.3) also moderated the symptoms of asthma in a mouse model.Conclusions
Local delivery of lentiviruses expressing IL‐5 and eotaxin shRNAs provides a potential tool in moderating airway inflammation and also has the potential for developing clinical therapy based on the application of shRNAs of chemokines and cytokines involved in T helper 2 cell inflammation and eosinophilia. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献46.
47.
zge Karayel Francesca Tonelli Sebastian Virreira Winter Phillip E. Geyer Ying Fan Esther M. Sammler Dario R. Alessi Martin Steger Matthias Mann 《Molecular & cellular proteomics : MCP》2020,19(9):1546-1560
Highlights
- •MS-based clinical assay that accurately determines phospho Rab10 occupancy.
- •Stable isotope labeled phosphopeptide injected as a standard with endogenous tryptic phospho Rab peptide for accurate ratio determination.
- •Determination of pRab levels in neutrophils of Parkinson disease patients.
- •Relevance of pRab levels as marker of PD.
48.
49.
50.